Eicom ENO-30, Руководство пользователя

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30 | P a g e
to go through the sample loop of the second valve, the sample would spread out and cause a
broadened peak.
7-2. Sample Amount
The ENO-30 accepts 1-50 µL of sample or standard. For accurate analysis, the same quantity of
sample or standard should be used. It’s best if the samples and standard are in the same
solutions. Use Carrier solution to dilute samples that are too high.
7-3. Autosampler Operation (optional)
The autosampler is primarily controlled via the Envision software (Eicom EPC-700), more details
refer to the Envision Software manual. For complete instructions on the use of the autosampler,
refer to the autosampler manual. There are some basic autosampler operations that are
covered here.
Before starting any analysis, make sure that the autosampler wash reservoir is filled with 10%
methanol. Water may also be used, but in that case, you need to pay extra attention to the
reservoir such that it does not have microbial growth. Change the water frequently and use soap
to clean.
Run the initial wash cycle to fill the syringe and wash the sample needle before starting any
analysis. During this wash cycle, check that there are no bubbles in the syringe. If there are
bubbles suck to the syringe plunger, they should be removed before starting the analysis. The
fastest way to remove the bubble may be to remove the syringe and let some fluid out past the
plunger by pulling the plunger nearly all the way out of the barrel. For more details, refer to the
autosampler manual.
During heavy use, it is recommended that the syringe and valve seal should be replaced
annually.