Eicom ENO-30, Руководство пользователя

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7. Sample Injection
Always use correct blunt ended needle to make injections or risk damaging the valve!
The standard syringe is a 25 µL Hamilton syringe (702SNR). However, a 50 µL syringe may also
be used (705SNR).
When the injector knob is moved between LOAD and INJECT, the flow pattern is switched. In
the INJECT position the flow from the pump passes through the sample loop and on to the
column. The syringe port is connected to one of the drain tubes. The knob should remain in the
INJECT position at all times when there is not a syringe inserted in the valve. In the LOAD
position, the loop is taken out of the flow and connected to the syringe port. The other end goes
to the other drain tube. Do not move the knob to the LOAD position unless there is a syringe in
the valve port. When the valve is switched back to the INJECT position, the sample is injected
and an electric signal is sent to trigger the autozero function and start data collection. You
should hear a beep. Do not connect any other tubing to the drain port in order to avoid to
potential troubles.
7-1. Manual Injector Operation
1. Aspirate the sample into a microsyringe. If an air bubble is generated inside of the syringe,
please flush out the solution and air bubble and slowly re-aspirate.
2. We recommend that the knob is left at INJECT before and after Injection.
3. Insert a syringe needle until you feel it stop (3 mm further than a weak resistance).
Don’t
depress plunger.
4. Slide the knob to LOAD and depress the plunger to load the sample into the sample loop
installed on the back of the manual injector. Slow smooth motion is required for best
results.
5. With syringe still in place, quickly and smoothly slide the knob from LOAD to INJECT
position. By moving the knob from LOAD to INJECT, an electrical signal is also generated
and you will hear a beep.
6. After completing these steps, remove the syringe and wash the syringe with super pure
water.
7. The needle port of the manual injector must be washed with super pure water using the
white port cleaner adapter attached to a syringe.
Caution; When there is second injection valve, such as an autosampler(AS-700) or online
injector (EAS-20s), installed between the manual injector and the column, the additional
injection valve should be set to the LOAD position. This will prevent the sample from going
through the sample loop of the second valve as it moves toward the column. If the sample were