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15. Appendices

15-1. Autosampler and Data Processor (EPC-700) Connection

1. Disconnect the signal cable of the manual injector (black twisted cable) from the connector

located just behind the injector.

2. Connect the AS-700 output (black and red wires from the 3-wire portion of the signal cable)

to the AUTOZERO/SIG. IN of ENO-30.

3. Connect the CPU of the ENO-30 to the Detector 1 of the EPC-700 data processor.

4. Connect the SIG.OUT of the ENO-30 to the INPUT 1 of the EPC-700 data processor.

5. Connect the INPUT 2 to the ERROR OUT on the Pump Unit and set the software to use

INPUT 1 and 2 to “Freeze”

Содержание ENO-30

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Страница 2: ...one 888 680 7775 FAX 858 560 8040 Email info eicom usa com Distributed by Eicom Europe Hilton House Ardee Rd Ground Floor Rathimines Dublin 6 Ireland Phone 353 1 902 2700 FAX 353 1 443 0784 Manufactur...

Страница 3: ...njector Tubing Connections 13 3 6 Connect Tubing to Autosampler optional 13 4 PUMP UNIT OPERATION 14 4 1 Pump overview 14 4 2 Power On 15 4 3 Key Pad 16 4 4 Display Screen 18 4 5 Log Function 18 4 6 A...

Страница 4: ...change 37 11 SEPARATION COLUMN NO PAK 38 11 1 NO PAK 38 11 2 Identification of Separation Quality 38 11 3 Washing the Separation Column 38 12 REDUCTION COLUMN NO RED 39 12 1 Structure and Maintenance...

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Страница 6: ...ses forced air and a Peltier device to maintain the columns reaction loop and detector cell at the correct temperature for the analysis The detector signal is taken from the side terminals into a data...

Страница 7: ...a safety hazard such as fire electric shock injury or other damage DO NOT OPERATE the ENO 30 until reading and understanding this instruction manual completely WARNING Before you operate the ENO 30 u...

Страница 8: ...exited first will not react So now there are two Nitrite peaks the first is from the Nitrite in the sample and the second is from the Nitrate in the sample Both are mixed with a Reactor solution supp...

Страница 9: ...oves dissolved air from the Carrier and Reactor solutions before they enter the pumps It does not work for large bubbles in the inlet tubing The degasser prevents bubbles from forming in the pumps whi...

Страница 10: ...lton syringe can be made here 7 Mixer Mixes the column effluent with the Reactor solution before entering the reaction coil 8 Reaction Coil Gives time for the reaction to be heated and go to completio...

Страница 11: ...nter the 3 way joint on opposite sides of the straight part of the joint to improve mixing Be careful to never let the Reactor solution enter the separation column It may be severely damaged Also turn...

Страница 12: ...bottle containing super pure water The outlet can be routed back to the same bottle or directly to waste Be sure to change this water frequently to prevent bacterial growth and the buildup of salts an...

Страница 13: ...s can cause the tubing to be crushed and the fluid passage blocked Flat Seal Some connections use a flat seal type of nut and ferrule white triangle in the figure Please check the direction of the fer...

Страница 14: ...then rotate no more than about 45 with a wrench Tubing installation Generally Eicom or its distributor will install the ENO 30 and connect all tubes In the event that you have to reconnect the tubing...

Страница 15: ...ture between 15 30 C during use Do not expose the directly to drafts including heating and air conditioning vents Keep away intense heat sources and other equipment that may produce strong magnetic fi...

Страница 16: ...rt is usually connected to the Detector 1 signal input of a data processor such as Eicom EPC 700 AUTO ZERO SIG IN An external device such as an autosampler or manual sample injector should be connecte...

Страница 17: ...he input channels of the autosampler here and have the input command set to Freeze the autosampler will stop making injections when the pump stops This way you avoid losing precious samples RUN STOP I...

Страница 18: ...mns Ports 5 and 6 are drain ports check the crystals don t form on the outlet as that can prevent proper manual injection volumes 3 6 Connect Tubing to Autosampler optional On the valve inside and tow...

Страница 19: ...k valve This valve also prevents solution from flowing from the pump back to the reservoir This is very sensitive be very careful to not over tightened when using a wrench The internal parts can be cr...

Страница 20: ...last time the pump was used and loads those upon startup Status Indicator The EP 700 indicator communicates the functional state of the instrument by color on off or blinking Indicator Subject Lightin...

Страница 21: ...16 P a g e 4 3 Key Pad Display Keypad...

Страница 22: ...n or stop of a gradient method MODE Switches between Isocratic Mode and Gradient Mode Only functional when two equal capacity pumps are installed TIMER ON OFF Start and stop of timer ESC Cancel parame...

Страница 23: ...sure fluctuation and quickly learns to compensate to reduce pulsing Please make sure to set this to PRESET when doing analysis 4 5 Log Function There is also a log function built in for recording the...

Страница 24: ...Pump 0 10 mL min Pressure Limit 10 MPa Set the Flow Rate Set the Carrier PUMP A flow rate to 0 33 mL min and Reactor PUMP B to 0 10 mL min Press SET ENTER key once Cursor appears next to PUMP A Set fl...

Страница 25: ...for both pumps to 10MPA Switch screen by pressing DISPLAY Press SET ENTER once Enter pressure limit with keys 1 0 Cursor moves to Pulse Free Mode Choose PRESET by repeatedly pressing SELECT Cursor app...

Страница 26: ...ou accidentally get in to the Gradient Mode GRA you need to switch back to the Isocratic Mode ISO Use the MODE key to switch back appears next to PUMP A to indicate that the pump is running and a pres...

Страница 27: ...open Now aspirate to remove fluid from the pump and pull fluid from the reservoir Please note If there is nothing connected to the downstream tubing this will not work Air will be pulled from the open...

Страница 28: ...an or replace it Check for leaks in the tubing and fix any you find ERROR 4 Motor Error Torque Overload Abnormal load detected at the pump drive motor Abnormal rotation in pump Remove air bubble from...

Страница 29: ...show the current readings from the detector such as Absorbance Press the Arrow and Enter keys and the same time to display the Absorbance value Remember the absorbance is calculated by using the diffe...

Страница 30: ...tamination disconnect the sample loop and wash the loop with HCl and water Super pure water is also the candidate of contamination removal It is better not to store the water always use fresh super pu...

Страница 31: ...owder Shake to dissolve Rinse the bottle the powder came in with the prepared Reactor B solution to dissolve last of powder and return to prepared solution Complete Reactor Solution Mix equal parts of...

Страница 32: ...should have low enough cadmium for normal disposal 6 7 Prepare for Analysis Check the amount of Carrier and Reactor Make fresh Carrier and Reactor as necessary For high sensitivity analysis all solut...

Страница 33: ...llect waste into small microcentrifuge tube for one minute If it is half the expected volume then the pump has a bubble and needs to be primed again to remove 7 Confirm that the Reactor and Carrier so...

Страница 34: ...ate the sample into a microsyringe If an air bubble is generated inside of the syringe please flush out the solution and air bubble and slowly re aspirate 2 We recommend that the knob is left at INJEC...

Страница 35: ...re covered here Before starting any analysis make sure that the autosampler wash reservoir is filled with 10 methanol Water may also be used but in that case you need to pay extra attention to the res...

Страница 36: ...ed 4 Manually flush the pump seal wash with the syringe 5 Make sure the inlet tubing and waste line are submerged 8 2 Complete Shutdown over 2 weeks 1 Stop the pumps Reactor first 2 Remove the columns...

Страница 37: ...irst 9 Leave the machine to run for 30 minutes and check the detector baseline by using the Acquisition Monitor in the Envision software 10 Inject appropriate standard to verify adequate sensitivity f...

Страница 38: ...ow switch the Reactor pump to 100 methanol and Then run the pump for 20 mins at 0 5 mL min 4 Switch the Reactor pump back to 10 methanol and aspirate 10 mL or more methanol from the drain valve Then r...

Страница 39: ...d so forth Please refer to unit 15 1 About sample preparation please refer to Eicom technical publication entitled Nitrite and Nitrate Analysis 10 1 Determining the Precolumn Condition If any of the f...

Страница 40: ...ing gel by adding about 30 mL of pure methanol to the bottle that the packing gel came in The exact amount of methanol isn t important If the bottle has been used before the methanol may have evaporat...

Страница 41: ...8 Remove the syringe from the outlet and connect it to the inlet side Fill the syringe with fresh methanol and flush the column to rinse the new packing material Then rinse with methanol twice more Th...

Страница 42: ...lace the pre column needs to be repacked again In most cases problems with packing are the result of voids in the packing material Please fill the precolumn slowly If the correct type of packing mater...

Страница 43: ...den and overlap To confirm that the problem is the separation column remove the precolumn and replace with a two way joint instead of the precolumn Make a standard injection If there is no change then...

Страница 44: ...duction column is around 1 3 months 12 3 Damage The reduction column loses its ability in following conditions Please avoid to injecting these samples AVOID injecting metals with lower ionization tend...

Страница 45: ...three way joint by the reaction loop Collect a sample here for 1 minute by setting the pump to 500 L min and running only the Reactor pump If it s close to 250 L there is a bubble 13 4 Removing Air Bu...

Страница 46: ...are details in video available on the Eicom users site or directly from your Eicom representative 13 4 High Carrier Pump pressure Check the guard column Remove the guard column and replace it with a t...

Страница 47: ...Nut 2Pump Head Washer 3Check Valve Inlet 4Check Valve Outlet 5Pump Head 6Piston Seal 7Backup Ring 8O Ring 9Pump Head Guide Nut 10Pump Head Guide Nut Washer 11Washing Port 12Pump Head Guide 13Piston 1...

Страница 48: ...ect water 2 3 mL in the middle of the piston opening Confirm that waters flows out the outlet check valve 4 Put a stopper fitting in the outlet valve Fill the pump head by injecting water until it ove...

Страница 49: ...is now complete and you can reinstall the pump head it you don t need to replace the piston Replacing them Piston 1 With the pump head removed loosen the screws holding the pump head guide in place Ag...

Страница 50: ...seal in place and slide the piston through the center hole and screw it in finger tight 8 Then use the piston replacement tool to secure the piston 9 Re attach the pump head guide and O ring 10 Tight...

Страница 51: ...46 P a g e 12 Tightens up pump head guide nuts by alternative between the two screws until they are completely seated 13 Reconnect the tubing to the check valves and the process is completed...

Страница 52: ...ector 2 Connect the AS 700 output black and red wires from the 3 wire portion of the signal cable to the AUTOZERO SIG IN of ENO 30 3 Connect the CPU of the ENO 30 to the Detector 1 of the EPC 700 data...

Страница 53: ...x 400D x 145H mm Pump Unit Type Two independent dual reciprocating pumps Flow Rate 1 750 L min Stroke 4 L replacement volume Pulse Damping Function Actively computer controlled based on pressure feedb...

Страница 54: ...precolumn should be changed after every 10 to 20 samples 3 Cell Culture The same applies as described in 1 above or by direct injection as below In the case of direct injection please change the prec...

Страница 55: ...rranty The manufacturer s warranty will be void under the following conditions 1 Failure to follow instructions and warnings relating to product s use 2 Repaired or altered by anyone other than Eicom...

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