Eicom ENO-30, Руководство пользователя

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15-3. Sample Preparation
1. Tissue Homogenate
Add methanol at a concentration of 2 mL/g tissue.
Homogenize (in an ice bath, preferentially).
Centrifuge at 10,000 G for 10 min (at 4ºC, preferentially).
Inject the collected supernatant.
Note: If the concentration is too high, add the same volume of carrier solution to the
supernatant.
2. Blood
Centrifuge at 500 G.
Collect the supernatant (serum or plasma).
Add the same volume of methanol.
Mix using a vortex for 10 sec.
Centrifuge at 10,000 G for 10 min.
Inject the collected supernatant.
Note: Plasma or serum can be directly injected but the precolumn should be changed after
every 10 to 20 samples.
3. Cell Culture
The same applies as described in #1 above or by direct injection as below. In the case of direct
injection, please change the precolumn frequently.
4. Urine
Dilute with carrier solution to 50 times the volume and then inject.
5. Saliva
Dilute with carrier solution to 10 times the volume and then inject.
Note: The precolumn content should be exchanged after every 50 samples (for samples
described in #1-5 above). This is a rough estimation.
6. Microdialysate
Direct Injection: In the case of microdialysate, 100 to 200 samples can be injected per
precolumn.
The precolumn content should be exchanged about each 50 sample (for sample #1-5, rough
estimate) but microdialysate (6) can be injected 100 to 200 samples for one precolumn.