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5. Detector/Oven Operation
5-1. Power Switch
The power switch is on the left side, not too far back and toward the bottom edge. As soon as
the Detector Unit is turned on, the oven will begin to heat. You should feel air blowing inside the
chamber. When the door is open, the heat is off, but the fan stays on.
5-2. Setting Temperature
The oven temperature should normally be set to 35 °C. If it is not, you can set it by pressing the
set key and then using the arrow key to change the temperature. Press the enter key to confirm.
When the temperature of the column oven reaches 35 °C, the green light on the front of the
instrument is lit.
5-3. Detector Readings
The display can also show the current readings from the detector, such as Absorbance. Press
the Arrow and Enter keys and the same time to display the Absorbance value. Remember, the
“absorbance” is calculated by using the difference between the reference light which does not
pass through the sample and the light intensity after passing through the sample. To see the
individual Reference and Sample light signals press the Arrow and Enter keys again for the
Sample value and more time for the Reference value. These values are helpful for confirming if
the detector is working properly.
5-4. Autozero Function
You can press the autozero key at any point to manual zero the detector, such as during the
stabilization period just after starting the instrument. Remember that the instrument will
automatically autozero ju
st after each injection of sample so it’s not necessary to manually
operate the autozero.
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