Step
Action
4
Insert the tray in to either the
Upper or Lower position. For more informa-
.
Note:
If using the
NP Lens, make sure that it is fully inserted into the tray position.
5
Close the instrument door.
Image a plate using the NP lens
To capture images of multi-well plates and petri dishes, use the optional
NP lens for
even quantitative measurements. To do this, use the following procedure.
Step
Action
1
Place the
tray guide inside the black tray.
2
Place the multi-well plate or petri dish into the cutout in the centre of the
tray guide.
3
Open the instrument door
4
Insert the tray into the lower tray position
5
Insert the
NP lens into the lower tray position on top of the tray.
Note:
The
NP lens must be inserted fully up to the stop position.
6
Close the instrument door.
Field of view
The available imaging view depends on which tray position is used and which accesso-
ries, if any, are used. The field of view is smaller and the exposure time is shorter in the
upper tray position.
The image below illustrates the difference between the different field of views for the
Lower (1) and Upper (2) tray positions. Shaded areas indicate the area of the high-
performance field of view.
5 Operation
5.2 Prepare the instrument
Amersham ImageQuant 800 Operating Instructions 29372604 AC
75
Содержание AKTA go
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