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Fluorescence Liquid Tests |
75
Filter-Based Fluorescence System
Optic Probe
To pass, the Detection Limit
must be less than or equal to:
Top
10 pM
Monochromator-Based Fluorescence System
Optic Probe
To pass, the Detection Limit
must be less than or equal to:
EX 485 nm,
EM 528 nm
Top/Bottom: 20 pM
Linearity Test
1.
Calculate the Mean of the four wells for each concentration in columns 1–5.
2.
Perform linear regression using these values as inputs:
Filter- and Monochromator-Based
Fluorescence System
x
y
1000
Mean of the 1000 pM wells
500
Mean of the 500 pM wells
250
Mean of the 250 pM wells
125
Mean of the 125 pM wells
62.5
Mean of the 62.5 pM wells
3.
Calculate the R-Square value; it must be
>= 0.950
to pass.
Fluorescence Polarization (FP) Test
1.
Using the raw data from the Parallel read:
•
Calculate the Mean Blank (wells A6–H6).
•
Calculate the Signal for each HPR well: Subtract the Mean Blank from its
measurement value.
•
Calculate the Signal for each LPR well: Subtract the Mean Blank from its
measurement value.
2.
Using the raw data from the Perpendicular read:
•
Calculate the Mean Blank (wells A6–H6)
•
Calculate the Signal for each HPR well: Subtract the Mean Blank from its
measurement value.
•
Calculate the Signal for each LPR well: Subtract the Mean Blank from its
measurement value.
Cytation 5
Содержание Cytation 5
Страница 13: ...Installation ...
Страница 31: ...14 Install the Imager Module 19 Cytation 5 ...
Страница 37: ...Getting Started ...
Страница 54: ...42 Getting Started BioTek Instruments Inc ...
Страница 55: ...Maintenance ...
Страница 66: ...54 Maintenance BioTek Instruments Inc ...
Страница 67: ...Instrument Testing ...
Страница 113: ...Specifications ...
Страница 122: ...110 Specifications BioTek Instruments Inc ...