Biotek Cytation 5, Инструкция по эксплуатации

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Dispense Module Tests | 97
6. Place a new 96-well microplate on the balance and tare the balance.
7. Place the plate on the microplate carrier.
Running a dispense procedure without placing a plate in the reader will result in
contamination of the reader from spilled liquid.
When each dispense step is finished, you will weigh the plate,
record the weight, tare the balance with the plate on it, and then
place the plate back on the carrier for the next step.
8. Select
Plate > Read
and click
READ
. Gen5 prompts you to empty the tip
priming trough.
9. When ready, click
OK
at the Load Plate dialog to begin the experiment. The
sequence is as follows:
a. Dispense 80 µL/well to columns 1–4.
b. Remove the plate and weigh it. Record the weight and tare the balance.
c. Place the plate on the carrier and dispense 20 µL/well to columns 5–8.
d. Remove the plate and weigh it. Record the weight and tare the balance.
e. Place the plate on the carrier and dispense 5 µL/well to columns 9–12.
f. Remove the plate and weigh it. Record the weight.
g. Manually pipette 150 µL of deionized or distilled water into all 12
columns, on top of the green test dye solution.
h. Place the plate on the carrier for a 15-second shake, the “80 µL” read at
405/750 nm, and the “20 and 5 µL” read at 630/750 nm.
10. When processing is complete, save the file with an identifying file name.
11. Remove the plate from the carrier and set it aside.
12. Repeat steps 5–11 using
Cytation 5 Disp 2 Test.prt
.
13. See the instructions for analyzing the results below.
14. When all tests are complete, prime both dispensers with at least 5000 µL of
deionized water to flush out the green dye solution.
Results Analysis
The pass/fail criteria for each set of 32 wells with the same dispense volume is based
on the calculated coefficient of variation (% CV) and Accuracy % Error.
For each volume dispensed (80 µL, 20 µL, 5 µL), for each dispenser (1, 2):
•
Calculate the Standard Deviation of the 32 wells
• Calculate the Mean of the 32 wells
• Calculate the %CV:
(Standard Deviation / Mean) x 100
•
Calculate the Accuracy % Error:
((Actual Weight – Expected Weight)/Expected Weight)* 100
Cytation 5