Biotek Cytation 5, Инструкция по эксплуатации

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Fluorescence Liquid Tests | 67
Analyze the Results
•
The plate is read five times in “Normal” position at 405 nm. Calculate the
Mean OD and Standard Deviation of those five reads for each well in
columns 1 and 2.
• For each well in columns 1 and 2, calculate the Allowed Deviation using the
repeatability specification for a 96-well pla te: ± 1% ± 0.005 OD from 0.000 to
2.000 OD (Mean * 0.010 + 0.005). For each well, its standard deviation should
be less than its allowed deviation.
•
The plate is read five times in the “Turnaround” position at 405 nm.
Calculate the Mean OD of those reads for each well in columns 11 and 12.
•
Perform a mathematical comparison of the Mean values for each microwell in
its Normal and Turnaround positions (that is, compare A1 to H12, A2 to H11,
B1 to G12,… H2 to A11). To pass the test, the differences in the compared
mean values must be within the accuracy specification for a 96-well
microplate: ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD .
Repeatability Specification
± 1% ±0.005 OD from 0.000 to 2.000 OD
± 3.0% ±0.010 OD from 2.000 OD to 2.500 OD
Fluorescence Liquid Tests
Required Materials
All Tests:
•
Deionized or distilled water
•
Various beakers, graduated cylinders, and pipettes
• 95% ethanol (for cleaning clear -bottom plates)
• Aluminum foil
•
(Optional, but recommended) 0.45-micron filter
•
(Optional) Black polyethylene bag(s) to temporarily store plate(s)
•
Gen5 protocols listed below and described on page 77
For the Filter-Based Fluorescence System
Cytation 5_FI_T_SF.prt
Corners, Sensitivity, and Linearity tests, using the top
optics
Cytation 5_FP.prt Fluorescence Polarization test
Cytation 5_TRF.prt Time-Resolved Fluorescence test
Cytation 5