Biotek Cytation 5, Инструкция по эксплуатации

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Empty/Clean the Tip Priming Trough | 51
This flushing and purging routine is also recommended before
disconnecting the outlet tubes from the reader, and before
decontamination to remove any assay residue prior to applying isopropyl
alcohol or sodium hypochlorite.
To flush the fluid path:
1. Fill two supply bottles with deionized or distilled water. Insert the supply
(inlet) tubes into the bottles.
2. Place the priming plate on the carrier.
3. From the Gen5 main screen, select
System > Instrument Control >
Cytation 5
.
4. Click the
Prime
tab and select
Dispenser 1
.
5. Set the Volume to
5000 µL
. Keep the default prime rate.
6. Click
Prime
to start the process. When the process is complete, carefully
remove the priming plate from the carrier and empty it.
7. Repeat the process for Dispenser 2.
Leave the water in the system overnight or until the instrument will be used again.
Purge the fluid from the system (see below) and then prime with the dispense
reagent before running an assay.
To purge the fluid from the system:
1. Place the inlet tubes in empty supply bottles or a beaker.
2. Select
System > Instrument Control > Cytation 5
.
3. Click the
Prime
tab and select
Dispenser 1
.
4. Set the Volume to
2000 µL
.
5. Click
Purge
to start the process.
6. When the purge is complete, repeat the process for Dispenser 2.
After purging the system, you may wish to run a quick Dispense protocol
to visually verify the dispense accuracy.
Empty/Clean the Tip Priming Trough
Applies only to Cytation 5 models with a dispenser.
The tip priming trough is a removable cup located in the rear pocket of the
microplate carrier, used for performing the Tip Prime. The trough holds about 1.5 mL
of liquid and must be periodically emptied and cleaned by the user. Gen5 will
instruct you to do this at the start of an experiment that requires dispensing.
1. Extend the microplate carrier and carefully remove the tip priming trough
from the carrier.
Cytation 5