LHC Protocols Explained | 57
LHC Protocols Explained
Prerequisite
This discussion about 405 LS protocols will be easier to follow if you are already
familiar with the LHC. Read "Understanding the LHC" in the Help.
Protocol Files
In addition to the "Protocol Steps" (the actions you tell the 405 LS to perform to
process plates), each protocol file contains "Instrument Settings."
The LHC must know an instrument's settings in order to create a protocol that will
run on that instrument. This virtual "Target Instrument Settings" feature lets you
write protocols when the instrument is not connected to your computer.
Generally, and especially when you are managing only one instrument, the best
practice is to always match the instrument settings to your instrument. (Select "
Get
actual settings"
from the connected instrument. Unless the instrument is not
connected to the computer, then, you must specify the settings.)
The "Instrument Settings" stored in the protocol file include the COM port and
configuration details like the type of manifold installed and other details that are
critical to controlling the instrument.
The LHC keeps track of the last-used COM port for an instrument type. For example,
when an EL406 runs a protocol, the LHC logs the COM port used and the next time an EL406
is used, the LHC applies the same COM port setting. You can disable this feature by defining
your
Ports
preference: select
Tools>Preferences>Ports
.
To correct the COM port for the current protocol, click the
Port
link and use the drop-down
list to select the correct value. The LHC stores the COM port value in the protocol file.
Managing Multiple Instruments
The target instrument settings feature is useful for those managing multiple
instruments. In addition to the flexibility of being able to create protocols for non-
connected instruments, you can create and save an instrument settings file for each
of your liquid handlers, another time saver.
BioTek Instruments, Inc.
Содержание 405 LS
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