Biotek 405 LS, Руководство оператора

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70 | Chapter 3: Operation
Add a secondary aspiration to a wash cycle
When too much residual is left in the wells during a wash cycle, you can add a
secondary aspiration to reduce it. Secondary aspiration can be performed
immediately after each aspiration in a wash cycle and/or after the final aspiration
step.
n
Secondary aspiration is also called Crosswise aspiration because it is typically
performed in a different location within the well. Reposition the tubes by
defining different X-, Y-, and/or Z-axis positions when enabling the secondary
aspiration for the most effective evacuation of the wells.
Edit the Wash step or stand-alone Aspirate step to add a secondary aspiration:
LHC Keypad
1. Double click the step in the
protocol to open it for editing.
2. Click
Advanced Options
(for
Aspirate) and enable
Perform
Secondary Aspirate
.
3. Apply a second aspiration to the
Final Aspirate
, also, for best
evacuation results.
4. Optionally, adjust the position of
the manifold as it addresses the
wells.
1. Edit the Wash step or stand-alone
Aspirate step:
Define>Edit>
select
protocol, and if necessary press
Options to scroll to the desired
step.
2. Select
ASPIR
and press Enter until
Secondary Aspirate is offered.
And/or select
OPTS>POST
to add a
second aspiration to the Final
Aspirate.
3. Select
YES
to perform a second or
crosswise aspiration.
4. Specify a crosswise position for the
manifold as it addresses the wells.
For example, you may want to adjust the X- or Y- axes to get to hard-to-reach areas
of certain types of wells. Or, you may want to lower the height (reduce the Z-axis),
to better evacuate fluid.
Run the Adjust Utility
: To determine the precise positioning of the dispense and
aspirate tubes.
Bottom Wash
Bottom washing adds an initial dispense/aspirate sequence, another wash cycle, to
the specified number of cycles. Fluid is simultaneously dispensed and aspirated to
405™ Microplate Washer LS