25
11.2. Cleaning, decontaminating and autoclaving
the lower part of pipettes
The lower parts of the
e
LINE single-channel
pipettes are autoclavable (see Fig. 8. Note: The
e
5000 model does not have parts no. 2, 3, and 5).
To clean, decontaminate or autoclave the lower
parts of the pipette follow these steps:
Disassembling:
1 . Remove the battery pack (1).
2.
e
10,
e
120,
e
300,
e
1000: Unscrew the tip ejector
collar (2) anti-clockwise and remove it.
e
5000: Unscrew the green tip cone (4) anti-
clockwise and remove it. Release the black tip
ejector assembly from the tip cone by turning it
anti-clockwise and then pull it out. Remove the
tip cone
fi
lter if
fi
tted and continue from Step 4.
3. Unscrew the tip cone holder (3) anti-clockwise and remove the tip cone
holder (3), tip cone (4) and spring (5). Remove the tip cone
fi
lter if
fi
tted.
4. Unscrew the exposed piston (6) anti-clockwise and remove it.
Cleaning:
To clean the exposed parts use Proline
®
Biocontrol, ethanol (70%),
isopropanol (60%) or mild detergent and soft lint-free cloth. The interior of
the tip ejector collar and tip cone can be cleaned with a cotton swab. Rinse
with distilled water if needed. Let the parts dry. Grease the piston thinly with
the grease provided.
Decontaminate:
For complete decontamination place
the tip ejector collar (2), tip cone holder
(3), tip cone (4), spring (5) and piston (6)
into a beaker containing Biohit Proline®
Biocontrol and leave for at least 30
minutes (Fig. 9), rinse the parts with
distilled water.
Note:
The corresponding parts in the
e
5000 model are the green tip cone (4),
the black tip ejector assembly and the
piston (6). Let the parts dry. Grease the
piston thinly with the grease provided.
Autoclaving:
The tip ejector collar (2), tip cone holder
(3), tip cone (4), spring (5) and piston (6) of the
e
LINE pipette can be steam
autoclaved (121°C, 1 bar overpressure for 20 minutes), see Fig. 8. These
parts can be autoclaved as one unit or separately as individual parts. It is
also possible to clean the parts and grease the piston prior to autoclaving.
Fig. 8.
2
3
4
5
6
1
Fig. 9.
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