Berthold LB 9510, Руководство по эксплуатации

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LB 9510 Lumat 4 Operation with ICE
71450BA2 Rev. 00, 12/2020 Page 23 of 79
content, single reporter gene expression, ac-
tivities of caspases, kinases and many other
enzymes.
[Endpoint Dual] In a Dual Label Assay two consecutive End-
point measurement series are defined. Each
series may start with an injection and/or a de-
lay. A mathematical ratio between both
measurement series may be calculated in the
[Multilabel Calculation Page] afterwards.
This measurement type is useful in lumines-
cent research assays to determine dual
reporter gene expression.
[Kinetic Single] The kinetic single mode is appropriate for fast
kinetics assays lasting over several seconds up
to minutes, e.g. enzyme kinetics and Calcium
influx.
[Flexible] This assay type allows a more flexible combi-
nation of Delay time, Dispense settings and
Endoint or Kinetic measurement steps.
[DualGlo] DualGlo is a Dual Label Assay with two consec-
utive Endpoint measurement series like
[Endpoint Dual] , but without injection. A
mathematical ratio between both measure-
ment series may be calculated in the
[Multilabel Calculation Page] afterwards.
This measurement type is useful in lumines-
cent dual assays with a stable signal (Glo
Assays).
Select your measurement type and click <Next>.
3.
Depending on the measurement type selected, the parameter in the
[General page] may vary.
As described in step 2, the definition of a second measuring step, the
selection of a multilabel calculation step, or special settings for Kinetic
measurements may be required. However, other parameter settings
can be omitted for some measuring types.