FlowStar2 LB 514
5. Analytic Splitter
45
When setting up the configuration, please check if the connected detector
(UV, IR, fluorescence, etc.) is capable of withstanding the backpressure
generated. If this is not the case, connect the detector after the
AS-xx
, i.e.
between
AS-xx
(FC output) and fraction collector (FC) (see Figure 12).
Higher flow rates (preparative range 4 - 20 ml/min) require a preparative
splitter; please specify your parameters: solvent, viscosity, flow rate, re-
quired splitting ratio.
For special applications or individual requests please contact our Bad
Wildbad headquarters or your local Berthold representative.
Figure 12:
Configuration with subsequently connected UV detector
If the backpressure created by the
AS-xx
should cause a problem for the UV de-
tector, you may connect the detector between
Analytic Splitter
and fraction col-
lector (
FC
).
The capillaries
C
and
FC
have an internal diameter of
0.1 mm
. The capillaries after
the T-mixer to the admixture cell should have an internal diameter of
1 mm
at a
total flow rate of
> 2 ml/min
, and
0.5
mm
at a total flow rate of
< 2 ml/min
. In
addition, the length of the supply line should be cut to
60 mm
when the flow rate is
< 1.5 ml/min
.
IN
85% 15%
FC
Cell
E
FC
C
UV-Detector
Column
LS-Pump
FlowStar LB 514
2
Status
READY
Cell
Test 14C
LS-Pump
OFF
Time
125.4
20
10
0
0
2
4
6
8
CPM
C-14
I
O
Status
READY
Cell
Test 14C
LS-Pump
OFF
Time
125.4
20
10
0
0
2
4
6
8
CPM
C-14
IN
OUT
T
LS
