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LXL-TB-015BB

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Figure 5

CsCl Gradients at Equilibrium for the SW 32.1 Ti

* Centrifugation of homogeneous CsCl solutions at maximum allowable speeds (from

Figure 4

) results in gradients

presented here.

1.20

1.10

1.00

1.30

1.40

1.50

1.60

1.70

1.80

1.90

Density (g/mL)

max

70.0

80.0

90.0

100.0

110.0

120.0

130.0

140.0

150.0

Distance from Axis of Rotation (mm)

min

64.4

162.8

SW 32.1 Ti ROTOR

= 20°C

= 4°C

20,000 RPM

25,000 RPM

30,000 RPM

32,000 RPM

25,00

0 RPM

3/4-filled

1/2-filled

1/4-filled

Содержание SW 32 Ti

Страница 1: ...5BB November 2011 Instructions For Use SW 32 Ti and SW 32 1 Ti Swinging Bucket Rotors For Use in Beckman Coulter Class H R and S Preparative Ultracentrifuges Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 ...

Страница 2: ...r by any means electronic mechanical photocopying recording or otherwise without prior written permission from Beckman Coulter Inc Find us on the World Wide Web at www beckmancoulter com Beckman Coulter Ireland Inc Mervue Business Park Mervue Galway Ireland 353 91 774068 製造販売元ベックマンコールター株式会社東京都江東区有明三丁目 5 番 7 号 TOC 有明ウエストタワー贝克曼库尔特有限公司美国加利福尼亚州 Brea 市 S Kraemer 大街 250 号邮编 92821 电话 001 714 993 53...

Страница 3: ...ntrifuge rotor system Their safety or reliability cannot be assured if used in an ultracentrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter ultracentrifuge that has been modified without Beckman Coulter s approval Alerts for Danger Warning Caution and Note DANGER DANGER indicates an imminently hazardous situation which if not avoided will result in death or serious injury WARNIN...

Страница 4: ...ascertained by Beckman Coulter Use of other manufacturers components or accessories in these rotors may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Attach all six buckets loaded or empty to the rotor for every run Make sure that filled containers are loaded symmetrical...

Страница 5: ...cription 3 Preparation and Use 4 Prerun Safety Checks 4 Rotor Preparation 4 Operation 6 Removal and Sample Recovery 7 Tubes and Accessories 7 Run Times 12 Run Speeds 13 Selecting CsCl Gradients 19 Adjusting Fill Volumes 19 Typical Examples for Determining CsCl Run Parameters 20 Care and Maintenance 21 Maintenance 21 Cleaning 22 Decontamination 23 Sterilization and Disinfection 23 Storage 23 Return...

Страница 6: ...vi ...

Страница 7: ... Symmetrically in the Rotor Buckets 6 2 Precipitation Curves for the SW 32 Ti Rotor r 15 3 CsCl Gradients at Equilibrium for the SW 32 Ti Rotor 16 4 Precipitation Curves for the SW 32 1 Ti Rotor 17 5 CsCl Gradients at Equilibrium for the SW 32 1 Ti 18 ...

Страница 8: ...s Tables 1 Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotor 8 2 Beckman Coulter Tubes and Accessories for the SW 32 1 Ti Rotor 9 3 Relative Centrifugal Fields for the SW 32 Ti and SW 32 1 Ti Rotors 14 ...

Страница 9: ...able tubes see Table 1 Nominal tube dimensions largest tube 25 89 mm Nominal tube capacity largest tube 38 5 mL Nominal rotor capacity 231 mL Approximate acceleration time to maximum speed rotor fully loaded 6 3 4 min Approximate deceleration time from maximum speed rotor fully loaded 9 1 2min Weight of fully loaded rotor 8 5 kg 18 7 lb Rotor and bucket material titanium a Relative Centrifugal Fie...

Страница 10: ...er of buckets 6 Available tubes see Table 2 Nominal tube dimensions largest tube 16 102 mm Nominal tube capacity largest tube 17 mL Nominal rotor capacity 102 mL Approximate acceleration time to maximum speed rotor fully loaded 6 1 2 min Approximate deceleration time from maximum speed rotor fully loaded 9 1 4 min Weight of fully loaded rotor 8 3 kg 18 3 lb Rotor and bucket material titanium a Rel...

Страница 11: ...tion Bucket and rotor body positions are numbered for operator convenience The rotor body and buckets are made of titanium finished with polyurethane paint Bucket caps are made of aluminum anodized for corrosion resistance Each bucket and cap assembly sets into grooves in the rotor body O rings made of Buna N rubber between each bucket and bucket cap maintain atmospheric pressure inside the bucket...

Страница 12: ...y safety officer Only the components and accessories listed in this publication should be used in these rotors Prerun Safety Checks Read the Safety Notice section at the front of this manual before using the rotor 1 Make sure that the rotor buckets and caps are clean and show no signs of corrosion or cracking 2 Make sure that the rotor is equipped with the correct overspeed disk a If the disk is m...

Страница 13: ...rease a Do not run a bucket without an O ring as the bucket will leak CAUTION Use only black caps 369643 on black SW 32 Ti buckets and red caps 369645 on red SW 32 1 Ti buckets 4 Use a lint free cotton swab to apply Spinkote lubricant 396812 to cap grooves in the bucket tops a Match bucket caps with numbered buckets b Align the pins on each side of the cap with the guide slots in the bucket c Twis...

Страница 14: ...Arranging Tubes Symmetrically in the Rotor Buckets NOTE Two three four or six tubes can be centrifuged per run if they are arranged in the rotor as shown All bucket assemblies must be attached to the rotor whether loaded or empty Operation For low temperature runs precool the rotor in the centrifuge or in a refrigerator before use especially before short runs to ensure that the rotor reaches the s...

Страница 15: ...s NOTE If the conical shaped adapters that support konical tubes are difficult to remove after centrifugation an extractor tool 354468 is available to facilitate removal 3 To remove the rotor from the centrifuge lift the rotor straight up and off the drive hub a Set the rotor on the rotor stand Tubes and Accessories The SW 32 Ti rotor uses tubes and accessories listed in Table 1 the SW 32 1 Ti rot...

Страница 16: ...00 g 202 25 83 mm 33 5 mL polyallmer Quick Seal bell top 344623 pkg 50 Norylb floating spacer 355536 32 000 RPM 175 000 g 192 25 89 mm 31 5 mL konicalpolyallomer open top 358126 pkg 50 Hytrelc adapter 358156 pkg 6 32 000 RPM 175 000 g 199 25 83 mm 28 mL konicalpolyallomer Quick Seal bell top 358651 pkg 50 adapter 358156 32 000 RPM 175 000 g 198 Noryl floating spacer 355536 25 76 mm 25 5 mL konical...

Страница 17: ...a Tube Required Accessory Max Speed RCF k factor Dimensions Nominal Volume Description Part Number Description Part Number 16 102 mm 14 5 mL polyallomer Quick Seal bell top 356291 pkg 50 Noryl floating spacer 355579 32 000 RPM 187 000 g 199 16 102 mm 17 mL Ultra Clear open top 344061 pkg 50 none 32 000 RPM 187 000 g 228 16 102 mm 12 5 mL konicalpolyallomer Quick Seal bell top 358653 pkg 50 adapter...

Страница 18: ...ensures a tight reliable seal that protects your samples 16 67 mm 10 5 mL polyallomer Quick Seal bell top 344622 pkg 50 Noryl floating spacer 355579 32 000 RPM 187 000 g 133 16 57 mm 8 mL polyallomer Quick Seal bell top 344621 pkg 50 Noryl floating spacer 355579 32 000 RPM 187 000 g 102 16 44 mm 6 5 mL polyallomer Quick Seal bell top 345830 pkg 50 Noryl floating spacer 355579 32 000 RPM 187 000 g ...

Страница 19: ...on These tubes are heat sealed and do not need caps however spacers are required on top of the tubes when they are loaded into the rotor buckets 1 Fill Quick Seal tubes leaving a small bubble of air at the base of the neck a Do not leave a large air space too much air can cause excessive tube deformation 2 Some of the tubes listed in Table 1 and Table 2 are part of the g Max system which uses a co...

Страница 20: ...lomer and Ultra Clear open top tubes should be filled as full as possible 2 or 3 mm from the tube top for tube support If necessary float mineral oil or some other low density immiscible liquid on top of the tube contents to fill the tube to its maximum volume Do not use an oil overlay in Ultra Clear tubes All opposing tubes for a run must be filled to the same level with liquid of the same densit...

Страница 21: ...two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 3 Do not select rotational speeds in excess of 32 000 RPM In addition speeds must be reduced under the following circumstances 1 If nonprecipitating solutions more dense than 1 2 g mL are centrifuged reduce the maximum allowable run speed according to the following equation EQ ...

Страница 22: ... 68 300 38 400 17 100 49 200 27 600 12 300 29 900 16 800 7 480 20 000 15 000 10 000 73 000 41 000 18 200 50 900 28 600 12 700 28 900 16 200 7 210 5 000 500 4 270 43 3 070 31 1 870 19 5 000 500 4 560 46 3 180 32 1 800 18 a Entries in this table are calculated from the formula RCF 1 12r RPM 1000 2 and then rounded to three significant digits RCF x g Relative Centrifugal Fields SW 32 1 Ti Rotor rmax ...

Страница 23: ... combinations that intersect on or below the solid curves ensures that CsCl will not precipitate during centrifugation Tube fill volumes are indicated on the curves SW 32 Ti ROTOR 20 C 4 C 1 20 0 1 10 1 30 1 40 1 50 1 60 1 70 1 80 Homogeneous CsCl Solution g mL 5000 10 000 15 000 20 000 25 000 30 000 Rotor Speed RPM 1 90 3 4 3 4 f u l l f u l l 1 4 1 4 1 2 1 2 ...

Страница 24: ... at maximum allowable speeds from Figure 2 results in gradients presented here 1 20 1 10 1 00 1 30 1 40 1 50 1 60 1 70 1 80 1 90 Density g mL rmax 70 0 80 0 90 0 100 0 110 0 120 0 130 0 140 0 152 5 Distance from Axis of Rotation mm rmin 3 4 filled 1 2 filled 1 4 filled 66 8 SW 32 Ti ROTOR 20 C 4 C 20 000 RPM 20 000 RPM 25 000 RPM 25 000 RPM 30 000 RPM 30 000 RPM 3 2 0 0 0 R P M 32 000 RPM ...

Страница 25: ...combinations that intersect on or below the solid curves ensures that CsCl will not precipitate during centrifugation Tube fill volumes are indicated on the curves SW 32 1 Ti ROTOR 20 C 4 C 1 20 0 1 10 1 30 1 40 1 50 1 60 1 70 1 80 Homogeneous CsCl Solution g mL 5000 10 000 15 000 20 000 25 000 30 000 Rotor Speed RPM 1 90 3 4 3 4 f u l l f u l l 1 4 1 4 1 2 1 2 ...

Страница 26: ...maximum allowable speeds from Figure 4 results in gradients presented here 1 20 1 10 1 00 1 30 1 40 1 50 1 60 1 70 1 80 1 90 Density g mL rmax 70 0 80 0 90 0 100 0 110 0 120 0 130 0 140 0 150 0 Distance from Axis of Rotation mm rmin 64 4 162 8 SW 32 1 Ti ROTOR 20 C 4 C 20 000 RPM 20 000 RPM 25 000 RPM 30 000 RPM 30 000 RPM 32 000 RPM 32 000 RPM 25 000 RPM 3 4 filled 1 2 filled 1 4 filled ...

Страница 27: ... speed The gradients in Figure 3 and Figure 5 can be generated from step or linear gradients or from homogeneous solutions But the total amount of CsClinsolutionmustbeequivalenttoahomogeneoussolutioncorrespondingtotheconcentrations specified in Figure 3 and Figure 5 Figure 3 and Figure 5 can also be used to approximate the banding positions of sample particles Curves not shown may be interpolated ...

Страница 28: ...oint in Figure 3 where each particle density intersects the curve corresponding to the selected run speed and temperature Particles will band at these locations across the tube diameter at equilibrium during centrifugation In this example particles will band about 120 and 125 mm from the axis of rotation about 5 mm of centerband to centerband separation To determine interband volume in milliliters...

Страница 29: ...mm from the axis of rotation about 9 5 mm apart Care and Maintenance Maintenance NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use 1 Frequently check the bucket O rings for signs of wear a Replace O rings every 6 months or whenever worn or damaged b Use the hooked removal tool...

Страница 30: ...e wash the rotor frequently at least weekly to prevent buildup of residues 1 Wash the rotor buckets O rings and caps in a mild detergent such as Beckman Solution 555 that won t damage the rotor a Dilute the detergent 10 to 1 with water The Rotor Cleaning Kit contains two plastic coated brushes and two quarts of Solution 555 339555 for use with rotors and accessories 2 Rinse the cleaned rotor and c...

Страница 31: ...mage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included with each box of tubes for tube sterilization and disinfection procedures Quick Seal Ultra Clear and thinwall open top tubes are disposable and shoul...

Страница 32: ...f the reported problem Use the address label printed on the RMA RGA form when mailing the rotor and or accessories Customers located outside the United States should contact their local Beckman Coulter office Supply List NOTE Publications referenced in this manual can be obtained at www beckmancoulter com by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local...

Страница 33: ...ick Seal Cordless Tube Topper kit 50 Hz Great Britain 358314 Quick Seal Cordless Tube Topper kit 50 Hz Australia 358315 Quick Seal Cordless Tube Topper kit 50 Hz Canada 367803 Tube racks for the Tube Topper for 16 mm diameter tubes for 38 mm diameter tubes 348123 348124 OptiSeal tube rack 361646 Tube removal tool Quick Seal tubes 361668 Extractor tool konical tube adapters 354468 Spinkote lubrican...

Страница 34: ...LXL TB 015BB 26 SW 32 Ti and SW 32 1 Ti Swinging Bucket Rotors Supply List ...

Страница 35: ...sequent to the purchase of a Swinging Bucket Rotor are warranted only for a term co extensive with that of the rotor for which the bucket sets are purchased 6 This warranty does not cover the failure of a Beckman Coulter rotor in a centrifuge not of Beckman Coulter manufacture or if the rotor is used in a Beckman Coulter centrifuge that has been modified without the written permission of Beckman C...

Страница 36: ...sation under the manufacturer s warranty Disclaimer IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Factory Ro...

Страница 37: ......

Страница 38: ...nic pdf by request Rotors and Tubes CD 369668 Rotors and Tubes for Tabletop Preparative Ultracentrifuges Rotors and Tubes for J2 J6 Avanti J Series Centrifuges Rotors and Tubes for Preparative Ultracentrifuges Rotor Safety Bulletin Chemical Resistances for Beckman Coulter Centrifugation Products Included with shipment of instrument Additional References Chemical Resistances for Beckman Coulter Cen...

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