Applied Biosystems 3730xl, Руководство пользователя

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5. Hold an empty tube or beaker under the exit fitting
to receive approximately 5 mL of waste. Flush the
trap by pushing steadily on the syringe plunger.
IMPORTANT! DO NOT USE EXCESSIVE FORCE.
Take approximately 30 seconds to flush 5 mL of
either distilled or deionized water through the trap.
6.
Close the fittings by turning each clockwise until the
fittings seal against the block. Close the Luer fitting
first, then close the Exit fitting.
IMPORTANT!
Do not over-tighten the fittings. The fittings require only enough
tightening to prevent water leaks. Excessive tightening can damage the fittings.
7.
Remove the syringe from the Luer fitting. Hold the fitting with one hand while
turning the syringe counterclockwise with the other hand.
Install the capillary array
• Capillary array, 96- or 48-capillary
• Lab wipes, lint-free
• Gloves
IMPORTANT!
Wear gloves when you handle the capillary array.
Use the Install Array wizard when changing capillary arrays to properly fill the
capillaries with polymer.
1.
In the navigation pane, select GA Instruments
4
ga3730
4
Instrument
name.
2.
At the top of the screen, select Wizards
4
Install Array wizard.
3.
Follow the instructions in the wizard to install the capillary array.
IMPORTANT!
When this wizard runs, it introduces polymer into the anode
buffer jar. Replace the anode jar buffer after you run this wizard. See “Fill the
anode buffer jar“ on page 33.
4. Perform a spatial calibration (see “Perform spatial calibration“ on page 91).
Required
materials
Install a new or
used capillary
array
Chapter 9 Maintenance
Install the capillary array
9
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide 123