Analytik Jena CyBio Well vario OL3381-25-300, Руководство по эксплуатации

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CyBio
®
Well vario Release: 2017 53
Initial Start-Up & Routine Start-Up Procedure
6.3.2 Accuracy Test
Accuracy defines the degree to which a measured dispensed volume (mean value of all
readings measured for a 96 well, 384 well or a 1536 well microplate) is in agreement
with a predefined (target) volume.
Materials & preparatory action:

Laboratory balance with 1 mg minimum resolution.

The selected laboratory balance must be subject to regular calibration (check for
calibration mark).

Lidded 96 well, 384 well or 1536 well flat-bottom microplate.

Accuracy testing is performed with de-ionized water (1 bar and 998 g/cm³).
Test sequence

Mount a reservoir with deionized water in a place on the carriage or the rotary
table.

Mount a microplate adapter in another place of the carriage or the rotary table.

Moisten tips using a rinsing procedure as follows: number of cycles – 20,
volume – 25 μ l

CyBio
®
Well vario 1536: number of cycles 10 – 20, volume – 5 μ l.
Tips dab off.

Accuracy testing is performed in manual ‘Pipetting’ mode. The appropriate vol-
ume is aspirated with an additional stroke of 0.5 μ l.

Weigh the microplate in empty and lidded state.

Unlid the plate and place it onto the adapter.

Dispense the desired volume into the microplate immediately thereafter.

Lid the microplate again.

Weigh the microplate in filled and lidded state.

The time interval between empty-state and filled-state weighing should not be
greater than 15 seconds.

Evaluate the measured results for variances of actual volume against nominal vol-
ume.

At least three readings should be taken for each volume.
NOTICE
Since new microplates, typically, are vacuum-packed, a selected microplate must be un-
packed at least one week before testing (the weight of a new microplate may decrease
or increase as a result of evaporation and absorption respectively).