Analytik Jena CyBio Well vario OL3381-25-300, Руководство по эксплуатации

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56 Release: 2017 CyBio
®
Well vario
Initial Start-Up & Routine Start-Up Procedure
6.3.4 Precision Test (Capillary Head)
To perform precision testing, use the following procedure.
Preparation
To wet an inserted capillary magazine, use process settings as follows:
Procedure:
Allow 8 seconds for taking in p-nitrophenol (in DMSO) by capillary force. Then dispense
this solution into a 384 well microplate with transparent bottom for 1 second at a pres-
sure of 25psi (1.72bar). Fill the microplate up with 50 μ l of 0.1N NaOH solution (e. g.
with a pipetting head). Place the microplate onto a plate shaker and let it shake for 10
min at 700 to 1000 r.p.m. Allow the plate then to rest for 10 min. Perform measure-
ment of absorbance at 405 nm after this rest time.
Rinse the microplate three times with water again after testing.
Result:
Typical values:
The p-nitrophenol solution filling should have a concentration of approximately
120 μ Mol for measurement. This is necessary to achieve an approximate absorption val-
ue of 0.6.
Rinse cycles: 3x
Rinsing liquid: Water
Aspiration 8 sec. by capillary force
Submergence depth of
capillaries for aspiration 4 mm
Dispensing 1 sec. by pressure (25 psi, 1.72 bar)
Dispensing gap: 1.5 mm
NOTE
This gap between the capillaries and the microplate must be kept in all cases. This pre-
vents pressure-dispensed liquid from spurting out.
Variation coefficient: (CV) < 10 %
Capillary magazine CV
25 nl < 9.0 %
100 nl < 3.0 %
250 nl < 2.0 %