Analytik Jena CyBio Well vario OL3381-25-300, Руководство по эксплуатации

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50 Release: 2017 CyBio
®
Well vario
Initial Start-Up & Routine Start-Up Procedure

Always use new tips for precision measurement. Rinse new tips before measure-
ment with p-nitrophenol working solution and settings as follows:

Testing is performed with the CyBio
®
Well vario in manual mode, using an aspira-
tion volume of 20 μ l or 10 μ l (
→
Table 9 on page 50 ), respectively, and a dispens-
ing volume of 10 μ l, 3 μ l or 2 μ l (
→
Table 9 on page 50 ), respectively.

Dispense the test volume into a microplate with prefilled NaOH solution (
→
Table 8 on page 49 ). For test volume dispensing, the pipette tips should sub-
merge into the prefilled NaOH solution by approximately 1 mm. Dispense the
remaining volume into the storage reservoir.
*Select a dye concentration corresponding to an extinction between 0.4 and 1.2 OD at 405 nm.
Table 9: Aspiration and test volumes

Tape the microplates after pipetting of the test volume and before shaking.

Use an orbital shaker for mixing of substances.
Table 10: Mixing times

Measure extinction in vertical photometer.

Evaluate measured data as a preparatory stage of CV value determination.

Compare obtained CV result with CV values in chapter
→
“Technical Specifications” .
Piston speed: 100 r.p.m.
Number of rinse cycles: 20
Rinsing volume: 10 μ l
CyBio
®
Well
vario
96-well/250
μ l
CyBio
®
Well
vario 96-well/
40
μ l/25 μ l
CyBio
®
Well
vario 384-well/
25
μ l
CyBio
®
Well
vario 96/384-
well/60
μ l
Aspiration vol-
ume 20 μ l 10 μ l 10 μ l 10 μ l
Standard test
volume* 10 μ l 2 μ l 2 μ l 3 μ l
p-nitrophenol
dye solution* 125 mg/L in
0.1 N of NaOH
600 mg/L in
0.1 N of NaOH
200 mg/L in
0.1 N of NaOH
125 mg/L in
0.1 N of NaOH
96-well microplate 384-well microplate
Orbital shaker of
700 rpm Shake for 15 min x
Orbital shaker of
1000 rpm x Shake for 10 min
Allow to rest for 30 to 45 min
Shake for 10 min