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Analytik Jena CyBio Well vario OL3381-25-300, Руководство по эксплуатации

Операционное руководство Analytik Jena CyBio Well vario OL3381-25-300 доступно для загрузки бесплатно на нашем сайте. Используйте это руководство для эффективной работы с продуктом. Скачайте его сейчас с manualshive.com и получите все необходимые указания по эксплуатации.

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104

Release:

 

2017

CyBio

®

 

Well

 

vario

Operation

 

The

 

achievable

 

degree

 

of

 

precision

 

is

 

limited

 

and

 

influenced

 

by:

the

 

wettability

 

of

 

tips,

the

 

dimensional

 

stability

 

of

 

their

 

outlet

 

opening,

the

 

way

 

the

 

system

 

and

 

involved

 

liquids

 

are

 

handled.

physical

 

and

 

chemical

 

properties

 

of

 

liquid

 

the

 

quality

 

of

 

microplates

You

 

are

 

also

 

advised

 

to

 

note

 

the

 

following:

NOTE

Liquid

 

is

 

aspirated

 

or

 

dispensed

 

as

 

a

 

result

 

of

 

underpressure

 

or

 

overpressure

 

created

 

by

 

piston

 

motion.

 

In

 

either

 

case,

 

the

 

process

 

will

 

not

 

be

 

completed

 

before

 

pressure

 

balance

 

state

 

has

 

been

 

reached.

 

The

 

time

 

required

 

to

 

reach

 

this

 

state

 

depends

 

-

 

among

 

other

 

factors

 

-

 

on

 

the

 

properties

 

of

 

the

 

liquid

 

being

 

handled.

 

For

 

this

 

reason,

 

adequate

 

pause

 

times

 

should

 

be

 

included

 

with

 

all

 

operating

 

sequences

 

as

 

necessary.

NOTE

When

 

working

 

with

 

dry

 

tips,

 

pressure

 

equalization

 

of

 

a

 

different

 

kind

 

will

 

occur

 

after

 

filling.

 

It

 

takes

 

a

 

longer

 

to

 

finish,

 

because

 

an

 

air

 

vapor

 

pressure

 

will

 

build

 

up

 

in

 

the

 

air

 

cushion

 

inside

 

the

 

tip

 

above

 

liquid

 

level,

 

resulting

 

in

 

liquid

 

emerging

 

from

 

the

 

tips.

 

This

 

can

 

be

 

prevented

 

by

 

triggering

 

a

 

few

 

initial

 

rinse

 

program

 

cycles,

 

until

 

the

 

air

 

inside

 

the

 

tip

 

is

 

saturated

 

with

 

vapor.

 

Even

 

after

 

a

 

longer

 

downtime

 

with

 

tips

 

in

 

filled

 

condition,

 

no

 

liquid

 

will

 

emerge

 

on

 

completion

 

of

 

preventive

 

rinsing.

NOTE

By

 

wetting

 

the

 

tips

 

with

 

liquid,

 

you

 

may

 

influence

 

the

 

achievable

 

level

 

of

 

precision

 

and

 

accuracy.

 

As

 

a

 

matter

 

of

 

importance,

 

tips

 

should

 

therefore

 

be

 

submerged

 

into

 

the

 

liquid

 

volume

 

just

 

as

 

much

 

as

 

necessary

 

for

 

a

 

particular

 

process.

 

In

 

addition,

 

the

 

depth

 

of

 

sub-

mergence

 

should

 

always

 

be

 

the

 

same

 

in

 

multi-cycle

 

mode.

 

This

 

can

 

be

 

achieved

 

by

 

maintaining

 

a

 

constant

 

reservoir

 

filling

 

level

 

with

 

the

 

help

 

of

 

the

 

sensor-controlled

 

res-

ervoir

 

pump.

NOTE

For

 

precise

 

work

 

results,

 

you

 

are

 

advised

 

to

 

use

 

a

 

non-shedding

 

slightly

 

moistened

 

scrubbing

 

towel

 

to

 

dab

 

the

 

tips.

 

You

 

may

 

place

 

the

 

towel

 

onto

 

a

 

standard

 

microplate

 

(for

 

example).

Содержание CyBio Well vario OL3381-25-300

Страница 1: ...Operating Manual CyBio Well vario...

Страница 2: ......

Страница 3: ...his documentation may be duplicated pho tocopied saved to a storage system or transferred to electronic media without prior written permission of the publisher Publisher Analytik Jena AG Konrad Zuse S...

Страница 4: ......

Страница 5: ...zus tzlich den Bestimmungen der europ ischen Richtlinien 2006 95 EG ber elektrische Betriebsmittel und 2004 108 EG ber elektromagnetische Vertr glichkeit is complying with all essential requirements o...

Страница 6: ......

Страница 7: ......

Страница 8: ......

Страница 9: ...ty Notes 17 3 8 Requirements On Operating Personnel 17 3 9 Safety Requirements for Transportation Installation 18 3 10 Safety Notes for Operation 18 3 10 1 General 18 3 10 2 Compressed Air Supply Only...

Страница 10: ...ransportation 45 5 2 Storage 46 6 Initial Start Up Routine Start Up Procedure 47 6 1 Site Requirements 47 6 1 1 Installation Requirements 47 6 1 2 Footprint Requirements 47 6 1 3 Energy Supplies 48 6...

Страница 11: ...Lubricating Transport Rails Linear Transporting Unit 117 9 2 3 Cleaning the Lifter 118 9 2 4 Tip Magazine Pipetting Head 118 9 2 5 Replacement of Capillaries 119 9 2 6 Cleaning the Carriage Linear Tra...

Страница 12: ...2017 CyBio Well vario A 1 3 Safety Notes 135 A 1 4 Product Versions 136 A 1 5 Installation Startup Procedures 137 A 1 6 Operation 139 A 1 7 Maintenance Care 140 A 1 8 Fault Removal 140 Declaration of...

Страница 13: ...symbols at the TWS module 36 Fig 21 Danger zones at TWS module 36 Abb 22 Vacuum set 37 Fig 23 TWS module with two peristaltic pumps 38 Fig 24 TWS module terminal points rearside panel 39 Fig 25 Perist...

Страница 14: ...g break 75 Fig 70 Display dispense tip change 75 Fig 71 Display dispense 76 Fig 72 Display dispense with position selection 77 Fig 73 Display dispensing start 77 Fig 74 Display tip change tip release...

Страница 15: ...ad change remove magazine adapter 96 Fig 110 Removal of magazine adapter head replacement from the back version 97 Fig 111 Display head change insert magazine 97 Fig 112 Insertion of tip magazine head...

Страница 16: ...XVI Release 2017 CyBio Well vario...

Страница 17: ...fety notes are marked with a pictogram and the appropriate signal word refer to section Safety Notes for the meaning of pictograms and signal words used herein They specify the type source and consequ...

Страница 18: ...sers are required to operate this system in no way or mode other than described or specified in this User Manual The system s range of applications is limited by the func tional scope of available sof...

Страница 19: ...its components Software protection is the sole responsibility of the user 1 4 Warranty Liability The period of warranty and scope of liabilities will be as stipulated under binding law and provided fo...

Страница 20: ...ry CyBio Well vario system delivery includes CyBio Well vario Transporting unit linear type or circular type Pipetting head or capillary head Compressed air control unit only for working with capillar...

Страница 21: ...nsporting unit 1650 x 772 5 x 370 mm Width x height x depth with 10 place circular transporting unit 700 x 772 5 x 895 mm Weight Basic unit without pipetting head 31 0 kg Pipetting head 14 0 kg Capill...

Страница 22: ...icient of variation 2 Precision in volume range of 5 to 25 l Coefficient of variation 1 Dosing volume of 96 piston pipetting head 2 5 l Randomly selectable in steps of 0 01 l Precision in volume range...

Страница 23: ...ge of 5 to 8 l Coefficient of variation 2 Capillary head Intended use Parallel transfer of compounds and paral lel dispensing into dry microplates Aspiration by capillary force effect Dispensing by je...

Страница 24: ...ations Formats SBS standard 96 384 1536 shallow well MP 96 384 1536 deep well MP Plate positions Linear positions 3 4 5 Positions at rotary table 10 NOTE The variation values quoted above can only be...

Страница 25: ...50 60 Hz Operating pressure only capillary head 1 72 bar 0 172 MPa 25 psi Protective fusing 230 V T 1 6 A 115 V T 3 15 A Power consumption 200 VA Interface RS 232 C Sub D 9 pin Current firmware versi...

Страница 26: ...10 Release 2017 CyBio Well vario Technical Specifications...

Страница 27: ...system operation from case to case generally established accident prevention industrial labour protection and environmental protection rules must be considered and duly followed A reference to potent...

Страница 28: ...a potentially dangerous situation that may result in death or severe injury crippledness unless prevented CAUTION Designates a potentially dangerous situation that may result in minor or insignificant...

Страница 29: ...ases Warns of crushing or pinching accidents Consider the pipetting head s weight for installa tion or deinstallation work The capillary head weighs 10kg 22lbs Warns of compressed air only capillary h...

Страница 30: ...io Well vario Safety Notes Fig 1 Safety labelling at pipetting head and capillary head 1 Warning note Weight 2 Warning note Magazine adapter Fig 2 Safety labelling at compressed air control unit only...

Страница 31: ...fety labelling provided at the system 3 6 Mechanical Danger Zones Fig 4 Danger zones at pipettor 1 Magazine clamping device 2 Lifter 3 Head cover Rearside view of system on both sides in head area wit...

Страница 32: ...acker operation Danger zones are Magazine clamping device Lifter Head cover Carriage with transport rails or rotary table Faulty action or misoperation may cause material damage or physical injury Alw...

Страница 33: ...n authorized service personnel Refrain from any kind of unauthorized rearrangements or changes in system setup notably such impacting the safety of personnel or the environment 3 8 Requirements On Ope...

Страница 34: ...vation of currently binding benchmark specifications and limit values applicable to the lifting and carrying of loads without the use of aids 3 10 Safety Notes for Operation 3 10 1 General Operating p...

Страница 35: ...erly trained in the handling of com pressed air 3 10 3 Explosion Proofness Fire Prevention The CyBio Well vario must not be operated in an explosive environment or using explosive substances Smoking o...

Страница 36: ...to clean exterior sur faces Use only original accessory items and original spare parts or such accessories and spare parts that are recommended by Analytik Jena AG refer to chapter Accessories Spare P...

Страница 37: ...he operation control panel Component Material Pipette tips PP 2 5 l ceramic tips Ceramic Capillary tips Glass PS polystyrene Piston seals1 1 Aerosols are likely to establish indirect contact between s...

Страница 38: ...re that the follow ing requirements are met Operating personnel must be aware of the locations and the proper handling of safety devices accident and hazard alarms as well as first aid kits and emerge...

Страница 39: ...hanisms are available CyBio Well vario with linear transporting unit CyBio Well vario with circular transporting unit Linear transporting unit The linear transporting unit is intended for motion of a...

Страница 40: ...o Well vario head change from the front version Nameplate Connection values sticker Fig 8 Nameplate connection values sticker Nameplate specifications Manufacturer details Product designation details...

Страница 41: ...r rotary motion of rotary table in horizontal direction System version for head replacement from the front The main switch for turning system power on and off is located on the front of the ba sic uni...

Страница 42: ...own to its zero position Keys for horizontal carriage or rotary table motion The effect on pressing of these keys depends on the current lifter position Vertical position 0 mm Horizontal motion to car...

Страница 43: ...ore conve nient removal or installation of a pipetting head the head has a handle attached to it and the magazine adapter provides a recessed handle Rails are located on the lateral sides of a pipetti...

Страница 44: ...28 Release 2017 CyBio Well vario Technical Description Fig 10 Pipetting head with magazine adapter 1 Rail 2 Handle 3 Pipetting head 4 Magazine adapter 1 2 3 4...

Страница 45: ...gnized when inserted into the basic unit by means of firmware Desig nation Identifica tion Volume Material Tips Drawing number Pos 4 Fig 111 1 For detecting the type TipTray when inserted into the pip...

Страница 46: ...e system front side front side or its rearside back side depending on the particular product version see fig 12 For convenient removal from or insertion into the system the capillary head has a specia...

Страница 47: ...ne Capillary Head A magazine contains 96 or 384 capillaries A capillary consists of glass This means that a sample will only make contact with this inert material Depending on their assigned fixed vol...

Страница 48: ...l Fig 16 Connector panel for head replacement from the front version 1 Warning label 2 Information label 3 Connector panel 1 PSPL SV terminal for external device 2 COM RS 232 Sub D socket 9 pole 3 Ter...

Страница 49: ...r Capillary Wash Station vacuum suction CAUTION Faulty pressure settings may damage the system Pressure settings made by Customer Service personnel must not be changed Make sure that the counterlockin...

Страница 50: ...One peristaltic pump fills washing liquid into the wash trough The washing liquid is suctioned away and the inner space of tips thus washed The peristaltic pump for outflow direction has a certain pr...

Страница 51: ...um set refer to page 37 Compressed air control unit refer to page 33 Suction trough OL5021 14 600 1 Wash trough 2 Outlet port 3 Inlet port 4 TWS module 5 Peristaltic pump for inflow 6 Peristaltic pump...

Страница 52: ...TWS module Danger Zones Fig 21 Danger zones at TWS module 1 Peristaltic pumps Do not place your hands or fingers into the peristaltic pump when open includ ing with objects as long as operation has no...

Страница 53: ...cuum unit SUP EXH VAC 2 Flask VAC Suction trough The vacuum unit consists of a vacuum pump and a waste bottle Vacuum is applied to withdraw liquid from the suction trough and transfer it to the waste...

Страница 54: ...complished by control software Fig 23 TWS module with two peristaltic pumps 1 TWS module 2 Tubing chart 3 Peristaltic pumps 4 Tube 5 Tube fixator 6 Lever NOTICE Aggressive acids leaches or organic sol...

Страница 55: ...closed 2 Peristaltic pump open 3 Tube fixed By moving a lever into the other position the given peristaltic pump can be opened or closed A tube can then be easily inserted or removed as necessary The...

Страница 56: ...els of the delivery pump left hand side rotate anticlockwise which means that liquid is pumped from right to left The right tube end must be submerged in the storage vessel for washing liquid the left...

Страница 57: ...CyBio Well vario Release 2017 41 Technical Description Fig 27 Peristaltic pump at TWS module direction of rotation and liquid flow...

Страница 58: ...tion of one or two stackers up to fifty microplates can be processed in a fully automated sequence per stacker Basic functions Explanation Dosing Aspirates a specified volume of liquid from a trough D...

Страница 59: ...ce In principle a sample transfer consists of dipping the capillaries into the liquid of a source plate and blowing the contents of the capillaries into a target plate Once the capillaries are submerg...

Страница 60: ...44 Release 2017 CyBio Well vario Technical Description...

Страница 61: ...n major temperature variations with likely formation of con densed water mechanical shocks and vibration should be prevented Use separate packing for transportation of pipettor head and magazine adapt...

Страница 62: ...after arrival of product shipment or is not required for a longer period of time it should preferentially be stored in its origi nal packing case Climatic requirements on facilities for system storag...

Страница 63: ...selection the following rules should be observed The operating room must have a stable horizontal dry and vibration free floor Do not install the system directly at doors or windows nor close to sour...

Страница 64: ...main power plug only into a mains socket with a grounded PE contact Make sure that the protection effect is not rendered ineffective by extension cables without a PE conductor or by the use of a volt...

Страница 65: ...e update is required Current firmware version on page 9 6 3 Function Tests 6 3 1 Precision Test Pipetting Head 96 384 Testing for variation coefficient CV per centage standard deviation is performed u...

Страница 66: ...2 OD at 405 nm Table 9 Aspiration and test volumes Tape the microplates after pipetting of the test volume and before shaking Use an orbital shaker for mixing of substances Table 10 Mixing times Measu...

Страница 67: ...into a microplate with prefilled NaOH solution For test volume dispensing the pipette tips should submerge into the prefilled NaOH solution by approximately 1 mm Dispense the remaining volume into the...

Страница 68: ...y stage of CV value determination Compare obtained CV result with CV values in chapter Technical Specifications on page 5 Individual variances 20 on completion of testing Result of leak testing on pag...

Страница 69: ...ips using a rinsing procedure as follows number of cycles 20 volume 25 l CyBio Well vario 1536 number of cycles 10 20 volume 5 l Tips dab off Accuracy testing is performed in manual Pipetting mode The...

Страница 70: ...Use caution to prevent sedimentation remaining in the membrane openings as you wipe the membrane clean Allow the membrane to dry Install tip magazine with new tips and clamp the tips Bring the reagent...

Страница 71: ...g tips on completion of testing CyBio Well vario 384 CyBio Well vario 1536 There must be no individual variances 20 on completion of testing A drop in the fill level of a tip suggests a potential leak...

Страница 72: ...n at 700 to 1000 r p m Allow the plate then to rest for 10 min Perform measure ment of absorbance at 405 nm after this rest time Rinse the microplate three times with water again after testing Result...

Страница 73: ...uires initial concentration values as follows Precision testing may also be combined with fluorescence measurement Capillary magazine Initial concentration of p nitrophenol in DMSO 25 nL 240 mMol 50 n...

Страница 74: ...58 Release 2017 CyBio Well vario Initial Start Up Routine Start Up Procedure...

Страница 75: ...nstalled the capillary head connect the head s compressed air supply tube to its terminal point at the compressed air control unit 3 Transfer power on off switch on the front panel for front side head...

Страница 76: ...The CyBio Well vario can be operated from the control panel which is located beside the system Once system power is turned on various menus will be available in manual control mode each of which prov...

Страница 77: ...provides further options for selection and execution of sub programs and system functions see fig 30 and Fig 31 respectively Fig 32 Display screen main menu Fig 33 Menu structure diagram view with cap...

Страница 78: ...stroke 1 Select aspiration volume Fig 34 Display screen dosing aspiration volume 2 Move liquid containg reservoir into position under the tips trough microplate Trigger carriage or rotary table motion...

Страница 79: ...r in raised position you can set the following positions with the help of or 5 Confirm established position via key in order to trigger a volume dispens ing cycle Fig 36 Display screen dosing dispensi...

Страница 80: ...for aspiration is 2 l above dispensing volume by default It can how ever be varied if necessary 3 Move a container with liquid into position under the tips trough microplate Trigger carriage or rotary...

Страница 81: ...o this trigger carriage or rotary table motion using or as neces sary to reach the required position Use to raise the lifter and use to lower the lifter Note With the lifter in raised position you can...

Страница 82: ...ion 10 In a dabbing break you can press and as necessary to bring the tips into a more convenient position for dabbing On termination of the dabbing break the pistons will return to zero position Fig...

Страница 83: ...spiration volume 4 Move a container with dispensing stock into position under the tips trough microplate Trigger carriage or rotary table motion for this purpose using or as necessary to reach the req...

Страница 84: ...for dispensing To do this trigger carriage or rotary table motion using or as neces sary to reach the required position Raise the lifter by pressing lower the lifter by pressing Note With the lifter i...

Страница 85: ...nto position under the tips for residual ejection 11 Trigger residual ejection by pressing key The differential amount of a total dispensing volume and an aspirated volume is dispensed once the cycle...

Страница 86: ...arriage or rotary table motion using or as neces sary to reach the required position Raise the lifter by pressing lower the lifter by pressing Note With the lifter in raised position you can set the f...

Страница 87: ...o reach the required position Raise the lifter by pressing lower the lifter by pressing Note With the lifter in raised position you can set the following positions via or 8 Confirm established positio...

Страница 88: ...plate with pipette tips is automatically released Fig 59 Display screen tip change tip release 2 Remove tip magazine from the pipettor Fig 60 Display screen tip change magazine removal CAUTION There i...

Страница 89: ...ips prompt appears on the screen Fig 62 Display screen tip change magazine insertion 4 Confirm clamped state by pressing key Fig 63 Display screen tip change tightening of tips 1 Magazine plate 2 Pipe...

Страница 90: ...er of rinsing cycles 2 Move a rinsing vessel into position under the tips for rinsing To do this trigger carriage or rotary table motion using or as neces sary to set the required position To raise th...

Страница 91: ...ed Capillary head There are two basic functions available in manual mode Dispensing Tip change Each individual action must be triggered by an operator The advantage is convenience of operation For exa...

Страница 92: ...must be included for 384 well or 1536 well processing The same applies in the case of 1536 well processing with a 384 channel capillary magazine If this is not the case continue with item 4 Always de...

Страница 93: ...change basic function in Manual menu The magazine plate with capillary tips is automatically released Fig 74 Display tip change tip release 2 Remove tip magazine from the pipettor 384X capillary maga...

Страница 94: ...h will be triggered and the screen display a Insert magazine with tips prompt Fig 77 Display tip change magazine insertion 1 Capillary magazine 2 Capillary tips CAUTION Prevent damage to the system On...

Страница 95: ...hould the pipettor be working in a different sub menu 2 Use key or in the main menu to select the Configuration submenu 3 Confirm your selection with the key You are working in the Configuration menu...

Страница 96: ...vessel with tips It also facilitates different applications for example the picking up of liquid over a cell lawn or the pipetting onto the bottom of a microplate from a preselected distance The heigh...

Страница 97: ...come down in point 1 of figure 56 Solution Set 1536 for plate type in the Configuration Wells menu Perform manual mo tion to position A for 1536 well microplates before dispensing point 3 in Figure 56...

Страница 98: ...ble speed A table speed setting will be valid for lifter motion and carriage and rotary table motion You can enter an end value for motion speed here Software settings can be made for end speed or sta...

Страница 99: ...a maximum available length of table motion lifter height This setting pre vents mechanical collision of a vessel with tips It also facilitates different applications for example the picking up of liq...

Страница 100: ...would come down in point 1 of figure 56 Solution Set 1536 for plate type in Configuration Wells menu Perform manual motion to A1 well position for 1536 well microplates before dispensing point 3 in fi...

Страница 101: ...r rotary table motion there may be overflow of liquid from the reagent troughs Select a reasonably reduced speed value if necessary NOTE In manual mode lifter motion is limited to a maximum speed of 1...

Страница 102: ...then confirm your selection with The magazine plate with pipette tips is automatically released Fig 85 Display main menu 2 Remove tip magazine from pipetting head and store magazine in tip tray pack a...

Страница 103: ...3 Use recessed handle to push the magazine adapter down into the pipetting head until mechanical stop position Fig 88 Display head change insert magazine adapter Fig 89 Insert magazine adapter head re...

Страница 104: ...er up until it engages mechanically Fig 92 Open cover head replacement from the front version 7 To retrieve the pipetting head or capillary head proceed as follows CAUTION There is danger of crushing...

Страница 105: ...93 Retrieval of pipetting head from the front head replacement version 8 To insert a new pipetting head or capillary head proceed as follows Hold pipetting head at its handle with one hand Use the oth...

Страница 106: ...automatically 10 Pull magazine adapter out of pipetting head from the front Use recessed handle at magazine adapter bottom for this purpose Fig 95 Display head change remove magazine adapter 1 Handle...

Страница 107: ...CAUTION There is danger of piston or system damage The pipetting head may only be loaded with magazines filled with tips or capillaries In sertion of a magazine is monitored by a microswitch If a maga...

Страница 108: ...m is ready again for operation Head replacement from the back To perform head replacement for from the back version proceed as follows 1 Press key or in the main menu as necessary to select the Head c...

Страница 109: ...ssed handle to push the magazine adapter down into the pipetting head until mechanical stop position Fig 102 Display head change insert magazine adapter Fig 103 Insertion of magazine adapter head repl...

Страница 110: ...ay head change remove head 6 Hold the handle at the back of the head cover with one hand then tilt cover up until cover mechanically engages Fig 106 Opening the cover at the back of the pipettor CAUTI...

Страница 111: ...g head head replacement from the back version 8 To insert a new pipetting head proceed as follows Hold pipetting head at its handle with one hand Use the other hand to support the head Note Use recess...

Страница 112: ...self The magazine adapter moves down and un locks automatically 10 Pull magazine adapter out of pipetting head from the front Use recessed handle at magazine adapter bottom for this purpose Fig 109 Di...

Страница 113: ...nsert magazine CAUTION There is danger of piston or system damage Insert only tip filled magazines into the pipetting head Insertion of a magazine is mon itored by a microswitch If a magazine is found...

Страница 114: ...powering on CAUTION There is danger of damage to the system due to incorrect parameter settings following a reset of operating parameters Operating parameters are preferably set by the customer servic...

Страница 115: ...parameters must be set manually again Drive parameters Are valid for powering of the pump motor piston drive the car riage rotable table motor and the lifter motor Starting r p m End r p m Number of s...

Страница 116: ...ceed as follows Washing procedure Solution for alkaline washing 10 RBS35 solution Carl Roth GmbH item no 9238 a Keep submerged in 10 RBS35 solution for 30 minutes submersion depth of capillary tips ab...

Страница 117: ...ke or is corrected by suitable compensatory motion dis pensing with additional stroke 7 4 1 Aspiration with Additional Stroke Fig 113 Aspiration with additional stroke This is the recommended standard...

Страница 118: ...leted by Motion into zero position A Move pistons into zero position command 6 triggers upward piston motion to their initial positions thus excluding the lower freeplay This process must be triggered...

Страница 119: ...volume or subvolumes Residual dispensing Motion to zero position Simplified cycle only for dispensing of single volumes Aspiration without additional stroke Dispensing with additional stroke Motion t...

Страница 120: ...er filling It takes a longer to finish because an air vapor pressure will build up in the air cushion inside the tip above liquid level resulting in liquid emerging from the tips This can be prevented...

Страница 121: ...head type Notify customer service 3 RAM fault Notify customer service 4 Tips are lose Turn power supply off and on again 5 Magazine not fully inserted Remove and insert magazine once again Tip magazi...

Страница 122: ...er unit drip catcher Turn power supply off and on again Note Repair work is necessary if error persists 18 Host computer triggered command that requires more parameters Check software for this com man...

Страница 123: ...r occurs repeatedly 30 Safety circuit interrupted special system configura tion Check safety circuit of exter nal devices 31 Error by external device Notify customer service 32 Error on float point op...

Страница 124: ...ordingly Command requires more parameters Command was transferred to pipettor with some required parameters miss ing Check transfer cables for proper condition Check that available soft ware version i...

Страница 125: ...nfiguration of serial interface and PC Error on horizontal drive motion Horizontal motion was obstructed by incorrectly seated microplate Remove microplate and mount it correctly No residual ejection...

Страница 126: ...a magazine had been installed No or faulty communica tion with X Y table Data transfer to table con troller interrupted Turn CyBio Well vario power supply shortly off and on again Pistons not in lower...

Страница 127: ...of a program in a location that is already occupied Delete program number then save a new program at this location Note The command which relates to this error is not supported in the given program Pi...

Страница 128: ...upper end position Use keyboard at the CyBio Well vario to trig ger table motion into low ermost position To do this press both direction keys at the same time This will automatically trigger a drive...

Страница 129: ...r Blocked or broken capillar ies Replace clogged or broken capillaries see section 9 2 5 No air supply or faulty con troller setting of com pressed air control unit Check air supply In the event of co...

Страница 130: ...pressure at out put Fixed opening clogged Replace component or fixed opening customer service Compressed air leak age at outlet opening Dust in sealing part of main valve Remove dust from valve seat...

Страница 131: ...ect to natural wear Similarly electronic components have no unlimited lifetime WARNING Note that contact with voltage carrying system parts may lead to physical injury or even death Turn system power...

Страница 132: ...y Clean transport rails with carriage or rotary table x Use mild cleaning agent or disinfectant to clean the tip washing trough x Wash tubing x Replace used tips x Clean tip magazine autoclave magazin...

Страница 133: ...ge or rotary table for visible damage and clean as necessary Remove tip magazine Turn power off Remove microplates or reservoirs x Only linear transporting unit Take carriage off and clean with mild c...

Страница 134: ...ng into contact with liquid and suffering contamination in such cases This may happen if liquid is drawn from vessels with insufficient filling level or if tips are not introduced deep enough Make sur...

Страница 135: ...the capillary magazine into its storage box The box provides a safe place of storage so individual capillaries can be replaced without any risk of breakage 4 Exchange individual capillaries as require...

Страница 136: ...place the carriage back onto the transport rails 7 Carefully push the tip magazine back into the holder at the pipetting head Make sure that tip magazine is correctly placed on completion of work 8 R...

Страница 137: ...eed with utmost caution in order to prevent unwanted position shifting of the sealing plate Otherwise there is danger of plate openings getting clogged with fluff balls 5 Do not remount the tips until...

Страница 138: ...122 Release 2017 CyBio Well vario Maintenance Care...

Страница 139: ...0 6 Only for capillary head Turn compressed air supply off and drain the system space until pressure relieved state is achieved 7 Turn system power supply off using main switch or socket bar for this...

Страница 140: ...124 Release 2017 CyBio Well vario Shutting Down NOTE A sub menu you had been working in when power was turned off will be restored when power becomes available again...

Страница 141: ...6 fs OL3381 24 750 CyBio Well vario 60 l pipetting head 96 fs OL3381 24 780 CyBio Well vario 250 l pipetting head 96 fs OL3381 24 830 CyBio Well vario 2 5 l pipetting head 384 fs OL3381 24 130 CyBio W...

Страница 142: ...DW NOTE For more detailed information about available pipette tips and magazines you should consult our latest catalog or visit us on the Internet at www analytik jena com 10 channel and 384 channel...

Страница 143: ...Wash Stations Capillary Toolset refer to chapter 9 2 5 CyBio Well vario 8 l pipetting head 1536 Consumable materials Wear parts NOTE Detailed information about available accessories can be contained...

Страница 144: ...128 Release 2017 CyBio Well vario Accessories Spare Parts...

Страница 145: ...workplace safety and environmental provisions of law NOTE Biological samples must be treated in accordance with locally binding regula tions for the handling of infectious material Hazardous substanc...

Страница 146: ...130 Release 2017 CyBio Well vario Waste Disposal...

Страница 147: ...e CyBio Well vario system as a whole It is only valid if used in combination with CyBio Well vario and Stacker User Manuals For specific information regarding the various modules you are referred to t...

Страница 148: ...132 Release 2017 CyBio Well vario...

Страница 149: ...ted The fill level of reser voirs is monitored by fill level sensors One CyBio Well vario is able to supply two stackers and one external module with electric power CyBio Composer software con trols C...

Страница 150: ...desk top device Make sure the laboratory bench has ade quate load bearing capacity compare with weight specifications before you install the system Phys dimensions Max width 1640 mm Height with stacke...

Страница 151: ...ed to follow all safety notes refer to chapter 3 in the CyBio Well vario User Manual and the Stacker User Manual 1 Stop and Down pushbutton for stacker 2 Warning text at peristaltic pump 3 Warning not...

Страница 152: ...to pipetting module Fig 121 Setup versions on base frame 1 Base frame 2 Mounting plate for pump module accessory item and extension module accessory item 3 CyBio Well vario 4 Stacker mounting plate in...

Страница 153: ...ervice personnel of Analytik Jena AG or that of specifi cally authorized contractors PSPL SW BC stacker left master bar code reader PC triple switch grounded contact in the rod grounded contact in the...

Страница 154: ...tube end must be submerged in the reagent vessel while the right tube end connects to the reservoir A 1 5 3 Inserting a Tube Into the Peristaltic Pump 1 Turn peristaltic pump lever to the left Pump is...

Страница 155: ...on refer to Plugin Pipettor Software ManualCyBio Com poser A 1 6 2 PC Mode PC interface Data transfers between the CyBio Well vario stackers additional modules and the control computer are accomplishe...

Страница 156: ...nal table that is provided below in order to check for all potential error sources Should difficulties persist or faults develop after this check other than described in the relevant module error tabl...

Страница 157: ...or quoted on page 9 of chapter Techni cal Specifications System reports tip maga zine not inserted error Tip magazine of older make back corners are not rounded Replace tip magazine Tip magazine edges...

Страница 158: ...142 Release 2017 CyBio Well vario...

Страница 159: ...aned and disinfected and are free of any infectious radioactive or otherwise hazardous or toxic substances and that no risk or danger is involved in servicing the device Name Description _____________...

Страница 160: ...144 Release 2017 CyBio Well vario...

Страница 161: ...ctor panel 32 Conventions 1 D Data transfers 139 Declaration of Safeness 143 Delay 80 103 Delivery of liquid 138 Detergent 51 Diluting 42 70 Dimensions 134 Direction of rotation 138 Dispensing 42 43 6...

Страница 162: ...filling station 138 Rinsing 42 74 Rotary table 23 S Safety notes 135 Scope of delivery 4 Sealing plate 121 Serial number III Setup versions 136 Shutting down 123 Site requirements 47 Source plate 43...

Страница 163: ...TWS module 35 38 Danger zones 36 Terminals 39 V Vacuum suction 33 Ventilation devices 18 Volume cycle 103 Volumes 27 30 W Waiting time 80 103 Warranty liability 3 Washing liquid 34 Washing trough 40...

Страница 164: ...148 Release 2017 CyBio Well vario...

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