Analytik Jena CyBio Well vario OL3381-25-300, Руководство по эксплуатации

Страница: 119 / 164

CyBio
®
Well vario Release: 2017 103
Operation
no volume is dispensed on excession of the upper freeplay (3). If dispensing motion cov-
ers a motion length that is greater than actually required for a given nominal volume,
the previously aspirated volume will be completely dispensed from the pipette tip, with
subsequent dispensing of air (D2).
A "Move pistons into zero-position" command (5) will move the pistons into their up-
per initial positions thus excluding the amount of lower freeplay.
This process must be triggered with no liquid present, i.e. the lifter must be in such a
position that the tips cannot make contact with liquid. Liquid which is aspirated in the
process may result in dosing errors or contamination of pistons.
7.4.3 Volume Cycle
Standard cycle:

Aspiration with additional stroke

Dispensing (single volume or subvolumes)

Residual dispensing

Motion to zero-position
Simplified cycle (only for dispensing of single volumes):

Aspiration without additional stroke

Dispensing with additional stroke

Motion to zero-position
7.4.4 Waiting Time
Piston motion always creates a state of underpressure or overpressure during an aspira-
tion or a dispensing cycle. In order to maintain the specified system precision, pressure
equalization in the tips must be allowed to finish. This is supported by a specific "Inter-
ruption" software command. A waiting time of two seconds proves long enough with
most watery solutions.
7.5 System Precision (Pipetting Head)
The CyBio
®
Well vario works based on the principle of air displacement. This means
that 96 or 384 pistons, which are mechanically connected with a common drive, per-
form motion in 96 or 384 air spaces created by the pipette tips and internal seals. The
volume resolution capability of piston motion corresponds to one tenth, one hundredth
or one thousandth of a micro liter.