Magnis and Magnis Dx NGS Prep System User Guide
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The system is no longer connected to the network.
✔
Make sure that a functioning ethernet cable is connected to the ethernet port at the back of the
instrument, and that the other end of the ethernet cable is connected to a live network port.
✔
“Set up the instrument IP address”
on page 29 for instructions on setting up the IP
address for the system. You may need to renew the IP address if one was assigned
automatically.
Auto-teaching failed.
✔
The barcode scanner may have failed to capture images of the teach points. See
on page 52 for instructions on cleaning the barcode scanner, then re-run
auto-teaching. If auto-teaching continues to fail, contact
✔
The teach points may be covered. Make sure the instrument deck has been cleared of all tip
boxes, plates, and strip tubes prior to starting auto-teaching. If auto-teaching continues to fail,
contact
Agilent Worldwide Technical Support
Pressing the power button on the front of the instrument fails to turn on power.
✔
Make sure that the power switch at the back of the instrument is in the ON position. If it is, then
make sure that the power cord is fully inserted into the power cord inlet, and that the other end
of the power cord is connected to a wall output that provides 100–240 VAC, 1000 W. If the
problem persists, contact
Agilent Worldwide Technical Support
The touchscreen displays
Time Remaining
of 0:00 at the end of run for some duration
of time without proceeding to completed run/sample collection screens.
✔
The Time Remaining value displayed on the touchscreen is only an estimate of time left in the
run, and this counter may remain at 0:00 for several minutes before the system is ready to
begin sample collection. This is not indicative of an issue with the run or the instrument.
Library/sequencing issues
Refer to the user manual for your specific Magnis Target Enrichment Kit for more tips on
troubleshooting issues with the library and sequencing data.
Library quality is low.
✔
Check if your DNA samples meet the guidelines for quality and concentration range specified in
the user manual for your Magnis Target Enrichment Kit. If the quality or concentration did not
meet the guidelines, then repeat the protocol using a DNA sample of adequate quality that is
within the recommended concentration range.
The sequencing reads do not cover the expected genomic regions.
✔
The wrong probe design may have been used in the protocol run for target enrichment. Review
the sample and probe tracking that was recorded during the run. Repeat the protocol run with
the correct probe design, if necessary.
