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1260 Infinity Binary LC - System User Guide

3

Optimization of the Agilent 1260 Infinity Binary LC

How to Achieve Higher Sensitivity

How to Achieve Higher Sensitivity

The sensitivity of a separation method is linked to the choice of stationary 
and mobile phases as good separation with narrow peaks and a stable 
baseline with minimal noise are desirable. The choice of instrument 
configuration will have an effect and a major impact is the setup of the 
detector. 

Содержание Agilent 1260 Infinity

Страница 1: ...Agilent Technologies Agilent 1260 Infinity Binary LC System User Guide...

Страница 2: ...ed only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and li...

Страница 3: ...s 3 Optimization of the Agilent 1260 Infinity Binary LC This chapter considers how to apply the theory and use the features of the LC system to develop optimized separations 4 System Setup and Install...

Страница 4: ...3 Optimization of the Agilent 1260 Infinity Binary LC 39 How to Configure the Optimum Delay Volume 40 How to Achieve Higher Injection Volumes 47 How to Achieve Shorter Cycle Times 48 How to Achieve Lo...

Страница 5: ...1260 Infinity Binary LC System User Guide 5 Contents 6 Appendix 101 Safety Information 102 Solvent Information 105 Agilent Technologies on Internet 110 Setting Up a Method using Edit Entire Method 111...

Страница 6: ...6 1260 Infinity Binary LC System User Guide Contents...

Страница 7: ...mponents 10 1260 Infinity Binary Pump G1312B 11 1260 Infinity High Performance Degasser G4225A 14 1260 Infinity High Performance Autosampler G1367E 15 1290 Infinity Thermostatted Column Compartment G1...

Страница 8: ...Binary LC are The configurable delay volume down to 120 L in the 1260 Infinity Binary Pump combined with a flow range from 0 05 up to 5 mL min at pressures up to 600 bar provides universal applicabil...

Страница 9: ...rsion for narrow bore columns Baseline robustness and fast spectral acquisition at data rates up to 80 Hz through the new optical design of the 1260 Infinity Diode Array Detector Different UV detector...

Страница 10: ...the needs of your individual application requirements A few configurations are described in more detail in this manual see System Setup and Installation on page 67 The modules that are described in th...

Страница 11: ...adient from one of two solvents per channel Active seal wash optional is available for use with concentrated buffer solutions Principle of operation The binary pump is based on a two channel dual pist...

Страница 12: ...System Components Figure 1 The Hydraulic Path of the Binary Pump with Damper and Mixer Ejbe ZVY 6 Ejbe ZVY 7 E hidch E hidch HZVah HZVah gdb WdiiaZ 6 hdakZci caZi kVakZ B m c 8 VbWZg DjiaZi kVakZ Djia...

Страница 13: ...mode For instructions on how to change between the two configurations see the G1312B Binary Pump User Manual Figure 2 The Hydraulic Path of the Binary Pump with Bypassed Damper and Mixer NOTE Bypassi...

Страница 14: ...pressure is measured by a pressure sensor The vacuum degasser maintains the low pressure by a controlled leak in the air inlet filter and a regulation of the vacuum pump using the pressure sensor The...

Страница 15: ...mpling sequence is not completed successfully an error message is generated Solvent is bypassed from the autosampler by the injection valve during the sampling sequence The needle moves to the desired...

Страница 16: ...ject and run step The six port valve is switched to the main pass position and directs the flow back through the sample loop which now contains a certain amount of sample The solvent flow transports t...

Страница 17: ...ispersion heat exchanger and each valve kit contains additional low dispersion heat exchangers for each column The low dispersion heat exchangers up to 4 can be mounted flexibly inside the TCC For con...

Страница 18: ...umns available for different applications Thus the column to be used becomes a simple method parameter This requires two 8 position 9 port valve heads one each in two of the TCCs Clustered TCC are rep...

Страница 19: ...caused by refractive index or thermal effects and results in more reliable integration of peak areas Figure 5 The light path through the DAD The module also incorporates electronic temperature contro...

Страница 20: ...h Signals can be output at 80 Hz 80 data points second for accurate recording of the fastest narrowest chromatographic peaks At the same time the module can also output full range spectra to the data...

Страница 21: ...e applications Each valve head comes as a complete kit containing all required capillaries additional low dispersion heat exchangers and other parts Some typical applications for Quick Change Valves a...

Страница 22: ...e a system that exactly meets your individual application requirements This individual configuration can be different from the standard configuration which is described in this System User Guide The p...

Страница 23: ...435 mm 7 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 74 W 253 BTU Maximum Ambient operating temperature 4 55 C 39 131...

Страница 24: ...eater pumping degassed H2O at 10 MPa 100 bar Pressure operating range Operating range 0 60 MPa 0 600 bar 0 8700 psi up to 5 mL min Pressure pulsation 2 amplitude typically 1 3 or 0 3 MPa 3 bar whateve...

Страница 25: ...Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak hand...

Страница 26: ...26 1260 Infinity Binary LC System User Guide 1 The Agilent 1260 Infinity Binary LC Product Description Specifications...

Страница 27: ...chnologies 2 Introduction Theory of Using Smaller Particles in Liquid Chromatography 28 Benefits of small particle size columns 34 Frictional Heating 37 This chapter gives an introduction to the Agile...

Страница 28: ...easing the flow rate without losing separation performance 2 Higher Resolution Long columns with sub two micron particles provide higher efficiency and therefore higher resolution which is required fo...

Страница 29: ...diffusion or multiple flow paths through the column B relates to molecular diffusion along the column axis longitudinal C relates to mass transfer of the analyte between the mobile and stationary phas...

Страница 30: ...analysis time is also reduced by a factor of two to three The increased efficiency is derived to a large extent from the reduction in multiple flow paths as a result of the smaller particles this lead...

Страница 31: ...mics of a separation can be considered and the properties of the solute and the stationary and mobile phases percentage of organic solvent ion strength and pH can be manipulated to achieve the shortes...

Страница 32: ...N selectivity retention factor k According to the resolution equation Figure 9 on page 32 the selectivity has the biggest impact on resolution Figure 10 on page 32 This means that the selection of ap...

Страница 33: ...contrast to totally porous silica columns these SPP columns have a solid core 1 7 m in diameter and a porous silica layer 0 5 m thickness surrounding it Speed and resolution of PoroShell columns are...

Страница 34: ...lent Technologies web site along with a method translator calculator www chem agilent com The results are presented graphically and in tabular form Shorter run times also deliver faster answers This i...

Страница 35: ...between particle size efficiency and analysis time Analysis time can be shortened without sacrificing column efficiency by optimizing particle size and pressure 8dajbc AZc i bb 8dajbc 8dajbc 8dajbc X...

Страница 36: ...such as impurity profiling can benefit from higher separation power Even the LC MS analysis of drugs in biological fluids can benefit from the higher peak capacity because of the reduced interference...

Страница 37: ...emperature gradient and therefore reduces the efficiency losses but a higher column outlet temperature has to be accepted The raised temperature may have an effect on selectivity At lower back pressur...

Страница 38: ...38 1260 Infinity Binary LC System User Guide 2 Introduction Frictional Heating...

Страница 39: ...ion for 3 and 4 6 mm i d Columns 45 How to Achieve Higher Injection Volumes 47 How to Achieve Shorter Cycle Times 48 How to Achieve High Throughput 50 How to Achieve Lowest Carry over 51 How to Achiev...

Страница 40: ...es and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with n...

Страница 41: ...columns require proportionally smaller extra column volumes to keep peak dispersion at a minimum In a liquid chromatograph the extra column volume will depend on the connection tubing between the aut...

Страница 42: ...illaries automatically using a 600 bar 2PS 6PT valve optional Table 4 Delay volumes of the 1260 Infinity Binary LC modules Components Delay Volume L Binary Pump1 1 in low delay volume configuration wi...

Страница 43: ...rtant to select the respective Enhanced Solvent Compressibility function in the auxiliary screen of the pump menu To minimize peak dispersion the low dispersion kit Low dispersion kit G1316 68744 must...

Страница 44: ...L mixer Low volume mixer 200 L 5067 1565 can be installed to reduce any residual mixing noise This small mixer gives the lowest UV baseline noise even under extreme gradient conditions See Figure 14...

Страница 45: ...mm inner diameter columns are about two and five times larger respectively than for the same length 2 1 mm i d columns and the flow rates that are used are also proportionally higher Therefore the sta...

Страница 46: ...or vice versa Figure Figure 16 on page 46 shows two overlaid chromatograms of a method for analysis of paracetamol and related impurities The method was transferred from an Agilent 1200 Series LC syst...

Страница 47: ...starting mobile phase and any increase will affect the separation particularly for early running peaks low retention factor In some cases it is the cause of peak distortion and the general rule is to...

Страница 48: ...ume mixer 200 L 5067 1565 Chromatographic conditions strongly depend on the compounds that are to be analyzed but some rules of thumb can be used to achieve short run times The flow rates should be as...

Страница 49: ...of the delay volume The lower the flow rate the greater the negative impact that can be expected from delay volume In Figure 17 on page 49 a 2 1 mm inner diameter column was used at a flow rate of 0 6...

Страница 50: ...e before the injection valve is switched to the bypass mode should be increased using the flush out factor to 20 This extends the time during which the autosampler delay volume is flushed with mobile...

Страница 51: ...ny application involving compounds liable to stick in the system The following functions of the autosampler can be used to minimize carryover Internal needle wash External needle wash Needle seat back...

Страница 52: ...ring aspiration of the sample that the injection valve is switched out of the flow path In this position the pump effluent is led directly to the column Prior to injection the outside surfaces of the...

Страница 53: ...s is described by the resolution equation where Rs resolution N plate count measure of column efficiency selectivity between two peaks k2 retention factor of second peak formerly called capacity facto...

Страница 54: ...rate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better s...

Страница 55: ...same but happens in half the time Any reduction in extra column volume will reduce dispersion and give better resolution This is already optimized in the 1260 Infinity Binary LC with narrow bore 0 12...

Страница 56: ...umn effluent should be cooled down to the temperature of the detector by using the 1 5 L cooler heater in the column compartment This ensures lowest noise level with UV detectors even at 5 mL min and...

Страница 57: ...alled in the column compartment For highest UV sensitivity the short mixer is also recommended See Figure 13 on page 43 and Figure 14 on page 44 The injection volume is also of importance especially i...

Страница 58: ...ate that is too low results in increased peak width and lower resolution see Figure 19 on page 58 Figure 19 Peak width vs UV data Rate Table 6 on page 59 shows the dependency of peak width resolution...

Страница 59: ...that elevated flow rates can also be advantageous for improved separation For 4 6 mm inner diameter columns packed with sub 2 micron particles a flow rate of 2 mL min is recommended as a starting val...

Страница 60: ...ty How to Achieve Higher Sensitivity The sensitivity of a separation method is linked to the choice of stationary and mobile phases as good separation with narrow peaks and a stable baseline with mini...

Страница 61: ...n of the peaks on the column This also creates peak dispersion and a lower peak height The data rate of the UV detector should be selected according to the actual peak width Higher than necessary data...

Страница 62: ...t can be injected on a smaller i d column then the dilution due to column diameter will be less and the sensitivity will increase For example decreasing the column i d from 4 6 mm to 2 1 mm results in...

Страница 63: ...owering the limit of detection LOD by a factor of about 3 depending on the application Table 7 Specifications for Max Light Cartridge Flow Cells Cartridge Cells Max Light Cartridge Cell 10 mm V s 1 0...

Страница 64: ...ists as 1290 Infinity Ultra Low Dispersion Kit 5067 5189 The cell should be part of the ultra low dispersion solution High Dynamic Range The Max Light Cartridge HDR cell can be used with the G4212A DA...

Страница 65: ...hase 50 mM in combination with high acetonitrile concentrations due to possible precipitation 6 An in line filter is recommended to catch particulates and extend column life Use the suitable 1260 Infi...

Страница 66: ...ion of the Agilent 1260 Infinity Binary LC How to Prevent Column Blockages Figure 20 Protection for 4 6 and 2 1 mm id columns packed with 1 8 m particles inlet frit with 0 2 m pore size chZgih l i hbV...

Страница 67: ...ling Software 68 Installing the Modules 70 Installing the System Modules 70 Integration Into the Network 70 Capillary and Tubing Connections in Flow Path 71 Priming the System 76 This chapter includes...

Страница 68: ...Guide 4 System Setup and Installation Installing Software Installing Software Installing the Software Controller and Data System For details of installation procedures for the software refer to the de...

Страница 69: ...ounters This allows a problem to be fixed before it impacts results The software includes an extensive suite of user information and documentation a set of calculators and tools to help set up calibra...

Страница 70: ...ling the System Modules For details of installation procedures for the modules refer to the individual module manuals These manuals also contain information on specifications maintenance and parts Int...

Страница 71: ...ation 4 Installing the Modules Capillary and Tubing Connections in Flow Path Depending on the system configuration capillaries of different lengths and diameters are used These are described below See...

Страница 72: ...ion Figure 21 on page 72 shows capillary and tubing connections in the flow path for the standard delay volume configuration of the 1260 Infinity Binary LC Figure 21 Capillary and tubing connections i...

Страница 73: ...0 17 mm x 700 mm S S Pump to cooled Autosampler 2 G1367 87304 Capillary ST 0 17 mm x 250 mm S S Autosampler to TCC 2 01090 87306 Capillary ST 0 17 mm x 380 nm S S Cooled Autosampler to TCC 3 G1316 87...

Страница 74: ...on Figure 22 on page 74 shows capillary and tubing connections in the flow path for medium and low delay volume configurations of the 1260 Infinity Binary LC Figure 22 Capillary and tubing connections...

Страница 75: ...ST 0 12 mm x 180 mm S S Autosampler to TCC 2 01090 87610 Capillary ST 0 12 mmx 280 mm S S cooled Autosampler to TCC or to item 3 if installed 3 G1316 80002 Heater long up 0 12 mm i d 1 6 L internal vo...

Страница 76: ...lvents and reagents can carry health risks Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sh...

Страница 77: ...channel A1 is advancing towards the pump If it isn t disconnect the solvent tubing from the solvent selection valve attach a syringe with a syringe adapter and pull the liquid through the degasser Rea...

Страница 78: ...d flow rate for your application and close the purge valve 5 Pump for approximately 10 minutes before starting your application When When the pumping system has been turned off for a certain time for...

Страница 79: ...rate for your application and equilibrate the system for approx 10 minutes prior to starting a run When When the solvent of a channel is to be replaced by another solvent that is not compatible solven...

Страница 80: ...solvent to flush air out of the system Miscible with almost all solvents After an installation Ethanol or methanol Alternative to isopropanol second choice if no isopropanol is available To clean the...

Страница 81: ...ine Plot 85 Purging the Pump 87 Data Acquisition in Method and Run Control View 88 Method Parameters for System Checkout with Isocratic Checkout Sample 88 Setting Up the Method 92 Running the Method f...

Страница 82: ...ysis using the columns and checkout sample that are provided with the Agilent 1260 Infinity Binary LC The example refers to menus and commands in OpenLAB CDS ChemStation Edition but identical function...

Страница 83: ...rt All Programs Agilent Technologies OpenLAB OpenLAB Control Panel Select the relevant instrument in the navigation pane under Instruments and click Launch online The ChemStation software opens in the...

Страница 84: ...Method and Run Control view of the ChemStation 2 On the menu bar select Method New Method and select DEF_LC M from the context menu Alternatively you can use the Load Method icon under the menu bar or...

Страница 85: ...eparing the System Configuring the Online Plot 1 If the Online Plot window is not visible Click View Online Signals Signal Window 1 to display the window 2 To configure the desired signal s in the Onl...

Страница 86: ...s is useful for checking the baseline and looking at previous injections The X and Y axis scales can be adjusted directly with the up down buttons on each axis The Adjust button in the Online Plot win...

Страница 87: ...few hours or more air may have diffused into the solvent lines and purging is recommended The solvent reservoirs are refilled and the pump requires purging to fill the system with fresh solvent If dif...

Страница 88: ...tic test mixture Agilent isocratic checkout sample 01080 68704 will be performed on the column ordered and delivered with your system Agilent isocratic checkout sample 01080 68704 contains the followi...

Страница 89: ...olvent B Acetonitrile Flow rate 4 0 mL min Solvent Composition 40 A 60 B Stop Time 1 min 1 min post time Autosampler Injection 1 L Column Compartment Column Column Eclipse Plus C18 4 6 x 100 mm 3 5 m...

Страница 90: ...parameters for first separation run low delay volume configuration Module Parameter Setting Pump Solvent A Water Solvent B Acetonitrile Flow rate 0 75 mL min Solvent Composition 40 A 60 B Stop Time 1...

Страница 91: ...e supplied syringe 3 Open the purge valve of the pump and purge each channel at 5 mL min This should be sufficient to remove air in the system 4 Install the supplied column into the column compartment...

Страница 92: ...d in the context menu a In the Method page for the 1260 Infinity Binary Pump enter the following parameters Flow rate 4 0 ml min Solvent A Select Water from the compressibility drop down list Solvent...

Страница 93: ...or the 1260 Infinity DAD enter the following parameters Use Signal Turn all signals except Signal A off by clearing the check boxes Signal A 254 nm bw 4 nm ref 550 nm bw 100 nm Peak width 0 02 b In th...

Страница 94: ...menu bar to load them 3 Place the sample vial in position 1 this is the front position of the 10 x 2 ml vial positions on the right hand side of the sample tray 4 Select Run Control Sample Info and e...

Страница 95: ...Start Guide 5 Data Acquisition in Method and Run Control View 6 The injection is made and the chromatogram appears in the Online Plot The data acquisition will stop when the Stop Time is reached The...

Страница 96: ...g the data to give quantitative and qualitative results This section looks briefly at integration and reports in data analysis so that the separations generated earlier in this chapter can be integrat...

Страница 97: ...lick Data Analysis in the bottom left of the screen 3 In the Navigation Panel find the data directory containing the data files All the single injection data are represented as a subset called Single...

Страница 98: ...icon to open the table as shown 3 Set Baseline Correction to Advanced for gradient runs 4 Set Slope Sensitivity to 5 Higher numbers will integrate steeper peaks and ignore less steep peaks 5 Set the...

Страница 99: ...1260 Infinity Binary LC System User Guide 99 Quick Start Guide 5 Data Analysis Specify the Report 1 On the menu bar click Report Specify Report to display the window shown in the figure below...

Страница 100: ...ally a directory The report file can be viewed directly in ChemStation or it can be found in the directory using the normal Windows File Explorer 4 Save the method once again to ensure the report sett...

Страница 101: ...rmation 105 Material Information 105 Agilent Technologies on Internet 110 Setting Up a Method using Edit Entire Method 111 Method Information 113 Instrument Acquisition 114 Data Analysis 129 Run Time...

Страница 102: ...andards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Safety Standards This is a Safet...

Страница 103: ...ailable at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this...

Страница 104: ...e damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not...

Страница 105: ...itions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agil...

Страница 106: ...ont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e...

Страница 107: ...ke nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against...

Страница 108: ...y strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with s...

Страница 109: ...used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To e...

Страница 110: ...ry LC System User Guide 6 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at...

Страница 111: ...r through the menu bar with its drop down menus A new method can be created by loading and editing an existing method or it can be created by loading and editing the blank template method DEF_LC M To...

Страница 112: ...nstrument curves Data Analysis comprises signal details integration parameters and reporting parameters Run Time Checklist comprises the parts of the method that will be executed NOTE During Edit Enti...

Страница 113: ...o be directly accessed through the menu Method Method Information or by right clicking on the graphical user interface This box allows information about the method to be entered This information will...

Страница 114: ...Binary Pump BinPump Thermostatted Column Compartment TCC Diode array Detector DAD Instrument Curves To move between the tabs click on the tab name at the top of the screen When parameter changes have...

Страница 115: ...er This is the recommended option and is configured in the next entry Needle Wash if selected above Mode determines how the outside of the needle will be rinsed either actively in the Flush Port or by...

Страница 116: ...e the sample from near the bottom of the vial With the HiP ALS for example a value of 0 would place the tip of the needle 1 mm above the bottom of a wellplate Equilibration time is a delay time betwee...

Страница 117: ...he column sooner Enable overlapped injection also switches the injection valve from mainpass to bypass after the injection has taken place either after the sample has flushed out of the injector or at...

Страница 118: ...s specialized injection procedures to be constructed which involve the manipulation of aliquots from multiple vials as for instance in pre column derivatization Reagent chemicals are automatically mix...

Страница 119: ...n Binary Pump tab Flow sets the flow rate up to 5 mL min For the example separation 4 mL minis used If the back pressure briefly reaches the maximum pressure setting the flow will be reduced for a few...

Страница 120: ...e composition of A and B in the mobile phase or if required to the flow rate and maximum pressure allowed The timetable makes linear changes to the parameters between the given setpoints The settings...

Страница 121: ...ters the Stop Time in the pump is considered to be the master and the other modules will usually be set to follow this value Post Time defines a countdown period after the end of a run during which th...

Страница 122: ...ary when the column is longer than 15 cm and needs to be supported by both sections The two sides can be operated separately when it is required to have two columns operating at different temperatures...

Страница 123: ...to 0 15 C down to 10 C below ambient although it should be noted that there are very few applications operating below 12 15 C Avoid using the TCC at such low temperature that condensation of water fro...

Страница 124: ...Array Detector tab Signals Up to eight separate signals chromatograms can be recorded To mark a signal for collection check the Use Signal box for that signal define the wavelength and bandwidth and...

Страница 125: ...d which takes B back down to the initial value over two minutes to gently start the re equilibration of the column No useful data is expected during this downwards ramp so the detector stop time is se...

Страница 126: ...sary to have signal A set as a broadband detector to ensure that peak spectra are available for all the different wavelength signals Store controls the spectral collection mode with the following opti...

Страница 127: ...flat lining at the bottom of the range However it should not be changed without good reason as making it more negative will increase baseline noise and reduce the range available for measuring positi...

Страница 128: ...llows monitored data streams other than detector signals to be stored with the data by checking the relevant box These are primarily used for diagnostic purposes They are Pump Pressure Flow A B Compos...

Страница 129: ...wer part of the screen You can select any or all of the acquired detector signals for processing If no signals are selected the table is empty in this situation ChemStation will default to processing...

Страница 130: ...130 1260 Infinity Binary LC System User Guide 6 Appendix Setting Up a Method using Edit Entire Method Figure 31 Signal details...

Страница 131: ...n the context menu In the Data Analysis view it can be accessed through the menu Integration Integration Events or the Edit Integration Events task icon Figure 32 Edit Integration Events screen Integr...

Страница 132: ...f the baseline needed to mark the start and end of a peak Peak Width the width at half height of the narrowest peak of interest should be entered This helps the integrator to distinguish between noise...

Страница 133: ...rt in the context menu In the Data Analysis view it can be accessed through the menu Report Specify Report or the Specify Report task icon Figure 33 Specify Report Screen To setup a simple area report...

Страница 134: ...t Size Time axis 100 of page Response axis 40 of page Destination Printer Checked Screen Unchecked File Checked File Setting PDF Checked Unique PDF file name Checked On the Quantitation settings tab C...

Страница 135: ...er Guide 135 Appendix 6 Setting Up a Method using Edit Entire Method Instrument Curves Figure 34 Instrument Curves screen The Instrument Curves checkboxes allow these recorded parameters to be overlai...

Страница 136: ...opportunity to link macro commands or programs into the work flow at various points In most cases the Data Acquisition and Standard Data Analysis check boxes are checked If no data analysis is requir...

Страница 137: ...sual configuration as the software always saves the method in the data file all versions since B 02 01 Only if the ChemStation has been configured such that Unique Sequence Folder Creation is turned o...

Страница 138: ...volume 45 configuring online plot 85 control and data evaluation 25 control assembly 14 cycle time instrument configuration 48 D data analysis 112 data analysis 96 data evaluation and control 25 DEF_L...

Страница 139: ...range 24 physical specifications 23 power consumption 23 pressure sensor 14 pressure operating range 24 pulsation 24 priming with a pump 78 principle of operation 14 purging 87 Q quick start guide in...

Страница 140: ...12 90303 Rev B www agilent com Agilent Technologies In This Book This manual contains information about the Agilent 1260 Infinity Binary LC The manual describes the following Product description intro...

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