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Agilent 1200 Series Autosampler Reference Manual

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Optimizing Performance

Optimization for Lowest Carry-over

Several parts of an injection system can contribute to carry-over:

•

needle outside

•

needle inside

•

needle seat

•

sample loop

•

seat capillary

•

injection valve

The autosampler continuous flow-through design ensures that sample loop,
needle inside, seat capillary, and the mainpass of the injection valve is always
in the flow line. These parts are continuously flushed during an isocratic and
also during a gradient analysis. The residual amount of sample remaining on
the outside of the needle after injection may contribute to carry-over in some
instances. When using small injection volumes or when injecting samples of
low concentration immediately after samples of high concentration, carry-over
may become noticeable. Using the automated needle wash enables the
carry-over to be minimized and prevents also contamination of the needle
seat.

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Содержание 1200 series

Страница 1: ...A Agilent 1200 Series Standard and Preparative Autosamplers Reference Manual ...

Страница 2: ...chnology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend Software and technical data rights granted to federal government customers include only those rights customarily provided to end user Customers of Software Agilent provides this customary commer...

Страница 3: ...ules built in troubleshooting and test functions 4 Repairing the Autosampler Instructions on simple routine repair procedures as well as more extensive repairs requiring exchange of internal parts 5 Parts and Materials Detailed illustrations and lists for identification of parts and materials 6 Introduction to the Autosampler An introduction to the standard and preparative autosamplers Theory of O...

Страница 4: ...4 Agilent 1200 Series Autosampler Reference Manual 9 Specifications Performance specifications of the Agilent 1200 Series Standard and Preparative Autosamplers ...

Страница 5: ...6 Installing the Thermostatted Autosampler 29 Flow Connections 34 Sample Trays 36 Choice of Vials and Caps 39 Transporting the Autosampler 42 2 Optimizing Performance 43 Optimization for Lowest Carry over 44 Using the Automated Needle Wash 45 Using an Injector Program 46 General Recommendation to Lowest Carry over 47 Fast Injection Cycle and Low Delay Volume 48 Overlapped Injection Mode 48 General...

Страница 6: ... Messages 57 Timeout 58 Shutdown 59 Remote Timeout 60 Synchronization Lost 61 Leak 62 Leak Sensor Open 63 Leak Sensor Short 64 Compensation Sensor Open 65 Compensation Sensor Short 66 Fan Failed 67 Open Cover 68 Restart Without Cover 69 Arm Movement Failed 70 Valve to Bypass Failed 71 Valve to Mainpass Failed 72 Needle Up Failed 73 Needle Down Failed 74 Missing Vial 75 Initialization Failed 76 Met...

Страница 7: ...termittent lock ups with or without vial in the gripper fingers 98 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the via 100 Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm 102 4 Repairing the Autosampler 105 Introduction into Repairing the Autosampler 106 Using the ESD Strap 107 Overvi...

Страница 8: ...lling the Sampling Unit 144 Injection Valve Assembly 147 Metering Drive Motor and Belt 150 Needle Drive Motor and Belt 152 Fan 155 ASM Board 157 Changing the Product Number or Serial Number 160 Using the Agilent ChemStation 160 Using the Instant Pilot G4208A 161 Using the Control Module G1323B 162 SUD Board 167 Power Supply 169 Leak Sensor 172 5 Parts and Materials 175 Main Assemblies 176 Sampling...

Страница 9: ... Draw Kit G1313 68711 195 900 µl Injection Upgrade Kit G1363A for G1329A 196 External Tray G1313 60004 197 6 Identifying Cables 199 Cable Overview 200 Analog Cables 202 Remote Cables 205 BCD Cables 210 Auxiliary Cable 212 CAN Cable 213 External Contact Cable 214 RS 232 Cable Kit 215 LAN Cables 216 7 Introduction to the Autosampler 217 Introduction to the Autosampler 218 Sampling Sequence 220 Injec...

Страница 10: ...t Sensors 235 Autosampler Main Board ASM 236 Firmware Description 241 Firmware Updates 242 Optional Interface Boards 243 BCD Board 243 LAN Communication Interface Board 245 Interfaces 247 Analog Signal Output 248 GPIB Interface 248 CAN Interface 248 Remote Interface 248 RS 232C 250 Setting the 8 bit Configuration Switch 252 GPIB Default Addresses 253 Communication Settings for RS 232C Communicatio...

Страница 11: ... 11 9 Specifications 259 Performance Specifications 260 A Safety Information 263 General Safety Information 264 Lithium Batteries Information 267 Radio Interference 268 Sound Emission 269 Solvent Information 270 Agilent Technologies on Internet 272 Index 273 ...

Страница 12: ...12 Agilent 1200 Series Autosampler Reference Manual Contents ...

Страница 13: ...logies 1 Installing the Autosampler Site Requirements 14 Unpacking the Autosampler 18 Optimizing the Stack Configuration 21 Installing the Autosampler 26 Installing the Thermostatted Autosampler 29 Flow Connections 34 Sample Trays 36 Choice of Vials and Caps 39 Transporting the Autosampler 42 ...

Страница 14: ...dules have a separate power supply and a power plug for the line connections The two modules are connected by a control cable and both are turned on by the autosampler module WARNING To disconnect the autosampler from line power unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off WARNING To disconnect the thermostatted autosampler ...

Страница 15: ...ench The instrument requires an additional 25 cm 10 inches of space on either side for the circulation of air and approximately 8 cm 3 1 inches at the rear for electrical connections Ensure the autosampler is installed in a level position If a complete Agilent 1200 Series system is to be installed on the bench make sure that the bench is designed to carry the weight of all the modules For a comple...

Страница 16: ... G2260A Type Specification Comments Weight 14 2 kg 31 3 lbs Dimensions height width depth 200 345 435 mm 8 13 5 17 inches Line voltage 100 120 or 220 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption apparent power Power consumption active power 300 VA 200 W Maximum Maximum Ambient operating temperature 4 55 C 41 131 F see on page 17 Ambient non operating temperatur...

Страница 17: ...terial for the carriers inspection Delivery Checklist Ensure all parts and materials have been delivered with the autosampler The instrument box contains the instrument and an Accessory kit A separate box contains the reference manual and the power cable In Table 2 on page 19 and Table 3 on page 20 are listed the content of each accessory kit Please report missing or damaged parts to your local Ag...

Страница 18: ...0 Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 inch 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 Finger caps x3 Reorder gives pack of 15 5063 6506 Front door cooled autosampler G1329 40301 Air channel adapter G1329 43200 C...

Страница 19: ...enches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 inch 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler G1329 40301 Air channel adapter G1329 43200 Tray for 15 x 6 ml vials x2 G1313 ...

Страница 20: ... a system you can ensure optimum performance by installing the autosampler in the stack in the position shown in Figure 1 on page 22 and Figure 2 on page 23 Figure 3 on page 24 and Figure 4 on page 25 show the configuration recommended for a thermostatted autosampler These configurations optimize the system flow path ensuring minimum delay volume ...

Страница 21: ...ference Manual 21 Installing the Autosampler 1 Figure 1 Recommended Stack Configuration for an Autosampler Front View Solvent cabinet Vacuum degasser Pump Column compartment Autosampler or Preparative Autosampler Detector Instant Pilot ...

Страница 22: ...er Reference Manual 1 Installing the Autosampler Figure 2 Recommended Stack Configuration for an Autosampler Rear View Remote cable CAN Bus cable to Instant Pilot CAN Bus cable LAN to LC ChemStation Analog signal to recorder AC power ...

Страница 23: ...ce Manual 23 Installing the Autosampler 1 Figure 3 Recommended Stack Configuration for a thermostatted ALS Front View Standard or Preparative Autosampler Degasser ALS Thermostat Column Compartment Detector Instant Pilot Pump Solvent Cabinet ...

Страница 24: ...Autosampler Figure 4 Recommended Stack Configuration for a thermostatted ALS Rear View LA N Li nk C onf i g I ni t Mode HP I B HP I B Addr ess LAN to LC ChemStation Analogsignal to recorder Remote cable CAN bus cable Autosampler Thermostat cable Analogsignal to recorder ...

Страница 25: ... see below and Cable Overview on page 200 Chemstation and or Control Module G1323B WARNING To avoid personal injury keep fingers away from the needle area during autosampler operation Do not bend the safety flap away from its position or attempt to remove the safety cover see Figure 5 Do not attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle Figure ...

Страница 26: ...ded in Recommended Stack Configuration for an Autosampler Front View on page 22 5 Ensure the power switch at the front of the Autosampler is OFF 6 Connect the power cable to the power connector at the rear of the sampler 7 Connect the CAN cable to the other Agilent 1200 Series modules 8 If an Agilent Chemstation is the controller connect either The GPIB cable to the detector The LAN connector to t...

Страница 27: ...d the autosampler is in a not ready condition and operation is inhibited NOTE The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF WARNING To disconnect the sampler from the line unplug the power cord The power will supply still uses some power even s...

Страница 28: ...Unpack the Sampler and the Thermostat Parts required Sampler and Thermostat Power cord for the other cables see below and Cable Overview on page 200 Chemstation and or Control Module G1323B WARNING Make sure that the condensation tube is always above the liquid level in the vessel If the tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further...

Страница 29: ...nt door and remove the transport protection foam 6 Place the Autosampler on top of the Thermostat Make sure that the Autosampler is correctly engaged in the Thermostat locks 7 Place the air channel adapter into the autosampler tray base Make sure the adapter is fully pressed down This assures that the cold airstream from the Thermostat is correctly guided to the tray area of the Autosampler Figure...

Страница 30: ...e between the Autosampler and the Thermostat see Figure 9 on page 33 11 Connect the power cables to the power connectors 12 Connect the CAN cable to the other Agilent 1200 Series modules Figure 8 Air channel adapter Air channel adapter WARNING Do not disconnect or reconnect the autosampler to ALS thermostat cable when the power cords are connected to either of the two modules This will damage the ...

Страница 31: ...ampler NOTE The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF WARNING To disconnect the sampler from the line unplug the power cord The power supply still uses some power even if the power switch at the front of the panel is turned OFF WARNING To a...

Страница 32: ...32 Agilent 1200 Series Autosampler Reference Manual 1 Installing the Autosampler Figure 9 Cable Connections GPIB RS232C CAN bus Remote Vial number output Relay contacts Thermostat Autosampler cable ...

Страница 33: ...e leak channel Preparation Sampler is installed in the LC system Parts required Parts from the Accessory kit WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or ha...

Страница 34: ...eries Autosampler Reference Manual 1 Installing the Autosampler Figure 10 Hydraulic Connections Column compartment inlet capillary Pump outlet capillary Solvent waste for corrugated waste tube Waste tube in Leak channel ...

Страница 35: ...s as required 3 Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 4 Press the front of the sample tray down to secure the tray in the autosampler NOTE If the thermostatted autosampler tray pops out of position the air channel adapter is not correctly inserted Figure 11 Installing the Sample Tray ...

Страница 36: ...s slightly different The vial positions of the right hand half tray begin at position 101 as follows Left hand 40 position tray 1 40 Left hand 15 position tray 1 15 Right hand 40 position tray 101 140 Right hand 15 position tray 101 115 Table 4 Supported trays for the Autosampler G1329A G2260A Description Part Number Tray for 100 x 2 ml vials G1313 44510 Halftray for 15 x 6 ml vials G1313 44513 Ha...

Страница 37: ...Agilent 1200 Series Autosampler Reference Manual 37 Installing the Autosampler 1 Figure 12 Numbering of Tray Positions Left hand half tray Right hand half tray Position 1 Position 101 ...

Страница 38: ...e 41 and Table 10 on page 41 shown with their Part numbers have been successfully tested using a minimum of 15 000 injections with the Agilent 1200 Series autosampler Table 5 Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass write on spot 2 5182 0543 5183 4492 5183 4494 Amber glass write on spot 2 5182 3376 5183 4493 5183 4495...

Страница 39: ...067 5183 2070 Clear glass write on spot 2 5182 0715 5183 2068 5183 2071 Amber glass write on spot 2 5182 0716 5183 2069 5183 2072 Table 8 Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE red rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE r...

Страница 40: ...ypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Clear PTFE red rubber 5182 3459 Table 10 Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red poly...

Страница 41: ...ion via carrier ensure The transport assembly is parked see Park Arm Park Gripper on page 89 The vial tray is secured If the autosampler is to be shipped to another location the transport assembly must be moved to the park position to prevent mechanical damage should the shipping container be subjected to excessive shock Also ensure the vial tray is secured in place with suitable packaging otherwi...

Страница 42: ...42 Agilent 1200 Series Autosampler Reference Manual 1 Installing the Autosampler ...

Страница 43: ...ard and Preparative Autosamplers Reference Manual Agilent Technologies 2 Optimizing Performance Optimization for Lowest Carry over 44 Fast Injection Cycle and Low Delay Volume 48 Precise Injection Volume 50 Choice of Rotor Seal 52 ...

Страница 44: ...e mainpass of the injection valve is always in the flow line These parts are continuously flushed during an isocratic and also during a gradient analysis The residual amount of sample remaining on the outside of the needle after injection may contribute to carry over in some instances When using small injection volumes or when injecting samples of low concentration immediately after samples of hig...

Страница 45: ...ial For best results the wash vial should contain solvent in which the sample components are soluble and the vial should not be capped If the wash vial is capped small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Injector Program with Needle Wash The injector program includes the command NEEDLE WASH When this command is included in the...

Страница 46: ...ure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are used For example Outside wash of needle in vial 7 before injection Injector program Draw x x y µl from sample NEEDLE WASH vial 7 Inject Wait equilibration time see text above Valve bypass Wait 0 2 min Valve mainpass Valve bypass Valve...

Страница 47: ...leaning In case the needle seat has got contaminated and carry over is significantly higher than expected the following procedure can be used to clean the needle seat Go to MORE INJECTOR and set needle to home position Pipette an appropriate solvent on to the needle seat The solvent should be able to dissolve the contamination If this is not known use 2 or 3 solvents of different polarity Use seve...

Страница 48: ...ching to mainpass until the actual run is finished You gain the sample preparation time when using this process Switching the valve into the bypass position reduces the system delay volume the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory ...

Страница 49: ...if overlapped injection is used To reduce the injection time the detector balance has to be set to OFF NOTE For the last injection of the sequence with overlapped injections it has to be considered that for this run the injection valve is not switched as for the previous runs and consequently the injector delay volume is not bypassed This means the retention times are prolonged for the last run Es...

Страница 50: ...mmended to ensure the mobile phase is adequately degassed Also using the automated needle wash see Optimization for Lowest Carry over on page 44 between injections reduces carry over to a minimum improving injection volume precision further Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may have an influence on the injection volume precision when ...

Страница 51: ...t speed to a higher value speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the plunger returns to the home position An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens the time required to run the injector program...

Страница 52: ...ons where the lifetime of the Vespel seal is drastically reduced a seal made of Tefzel is available see Injection Valve Assembly on page 184 Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under normal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extreme mobile...

Страница 53: ...e Autosamplers Reference Manual Agilent Technologies 3 Troubleshooting and Test Functions Overview of the Sampler s Indicators and Test Functions 54 Status Indicators 55 Error Messages 57 Maintenance Functions 84 Step Commands 93 Troubleshooting 95 ...

Страница 54: ...obable causes of the problem and a list of suggested actions to fix the problem are provided see Error Messages on page 57 Maintenance Functions The maintenance functions position the needle arm gripper assembly and metering device for easy access when doing maintenance see Maintenance Functions on page 84 Tray Alignment Tray alignment is required after repair of internal components or after a fir...

Страница 55: ...front of the autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Figure 13 Location of Status Indicators Status indicator Power supply indicator ...

Страница 56: ...ing an analysis run mode A yellow indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed for example front cover not installed or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the instrument has detected an internal ...

Страница 57: ...requires attention before the analysis can be continued for example repair exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument log book This section describes the meaning of autosampler error messages and provides information on probable causes and suggested actions how to...

Страница 58: ...he analysis was completed successfully and the timeout function switched off the pump as requested A not ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Suggested Actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required ...

Страница 59: ...tes the error message Probable Causes Leak detected in an external instrument with a remote connection to the system Shut down in an external instrument with a remote connection to the system The degasser failed to generate sufficient vacuum for solvent degassing Suggested Actions Fix the leak in the external instrument before restarting the autosampler Check external instruments for a shut down c...

Страница 60: ...he analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable Causes Not ready condition in one of the instruments connected to the remote line Defective remote cable Defective components in the instrument showing the not ready condition Suggested Actions Ensure the instrument showing the not ready condition is installed correctly...

Страница 61: ...nitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable Causes CAN cable disconnected Defective CAN cable Defective main board in another module Suggested Actions Ensure all the CAN cables are connected correctly Switch off the system Restart the system and determine which module or modules are no...

Страница 62: ...orithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the ASM board Probable Causes Loose fittings Broken capillary Leaking rotor seal or needle seat Defective metering seal Suggested Actions Ensure all fittings are tight Exchange defective capillaries Ex...

Страница 63: ...ensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable Causes Leak sensor not connected to the ASM board Defective leak sensor Suggested Actions Ensure the leak sensor is connected correctly Exchange the leak sensor ...

Страница 64: ...short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable Causes Defective leak sensor Suggested Actions Exchange the leak sensor ...

Страница 65: ...en circuit The resistance across the temperature compensation sensor NTC on the ASM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable Causes Defective ASM board Suggested Actions Exchange the ASM board...

Страница 66: ...short circuit The resistance across the temperature compensation sensor NTC on the ASM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable Causes Defective ASM board Suggested Actions Exchange the ASM board ...

Страница 67: ... hall sensor on the fan shaft is used by the ASM board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 seconds the error message is generated Probable Causes Fan cable disconnected Defective fan Defective ASM board Suggested Actions Ensure the fan is connected correctly Exchange fan Exchange the ASM board ...

Страница 68: ...removed The sensor on the ASM board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable Causes The top foam was removed during operation Foam not activating the sensor Sensor defective Suggested Actions Replace the top foam Exchange the ASM board ...

Страница 69: ...h the top cover and foam open The sensor on the ASM board detects when the top foam is in place If the autosampler is restarted with the foam removed the autosampler switches OFF within 30 s and the error message is generated Probable Causes autosampler started with the top cover and foam removed Suggested Actions Replace the top cover and foam ...

Страница 70: ...receive the correct position information from the encoders within the time window the error message is generated See figure Transport Assembly on page 226 for axes identification Arm Movement 0 Failed X axis Arm Movement 1 Failed Z axis Arm Movement 2 Failed Theta gripper rotation Arm Movement 3 Failed Gripper gripper fingers open close Probable Causes Mechanical obstruction High friction in trans...

Страница 71: ...the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated Probable Causes Defective injection valve Defective ASM board Suggested Actions Exchange the injection valve Exchange the ASM board ...

Страница 72: ... the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated Probable Causes Defective injection valve Defective ASM board Suggested Actions Exchange the injection valve Exchange the ASM board ...

Страница 73: ...oard The sensor detects the successful completion of the needle movement to the upper position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable Causes Defective or dirty position sensor Defective motor Sticking spindle assembly Defective ASM board Suggested Actions Exchange the sampling unit flex board Ex...

Страница 74: ... seat position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable Causes Needle installed incorrectly or wrong needle type too long Defective or dirty position sensor Defective motor Sticking spindle assembly Defective ASM board Suggested Actions Ensure the correct needle type is used and installed correctl...

Страница 75: ...e vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable Causes No vial in the position defined in the method or sequence Incorrect gripper alignment Defective gripper assembly defective gripper fingers or belt Defective transport assembly flex board Suggested Actions Install the sampl...

Страница 76: ... the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable Causes Mechanical obstruction Defective sampling unit flex board Defective transport assembly flex board Defective sampling unit motor Defective ASM board Suggested Actions Ensure unobstructed move...

Страница 77: ...nitors the home position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated Probable Causes Dirty or defective sensor Broken plunger Defective metering drive motor Defective ASM board Suggested Actions Exchange the sampling unit flex board Exchange the metering plunger and seal Exchange the meteri...

Страница 78: ... motor for gripper fingers The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable Causes Mechanical obstruction High friction in the t...

Страница 79: ...ampler checks correct operation of the gripper by closing and opening the gripper fingers while monitoring the motor encoder If a vial is still in the gripper when initialization is started the gripper fingers cannot close causing the error message to be generated Probable Causes Vial still in gripper Suggested Actions Remove the vial using the Release Vial function in the user interface Reinitial...

Страница 80: ...the needle seat to inject sample the safety flap locks into position Next and the gripper checks the safety flap by trying to move the safety flap away from the needle If the gripper is able to move beyond the safety flap position safety flap not in position the error message is generated Probable Causes Safety flap missing or broken Suggested Actions Exchange the safety flap ...

Страница 81: ...quence no vial should be held by the gripper The autosampler checks if a sample vial is stuck in the gripper by closing and opening the gripper fingers while monitoring the motor encoder If the gripper fingers are unable to close the error message is generated Probable Causes Vial still in gripper Suggested Actions Remove the vial using the Release Vial function in the user interface Reinitialize ...

Страница 82: ... monitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable Causes No wash vial in the position defined in the method Suggested Actions Install the wash vial in the correct position...

Страница 83: ... If the vial position does not exist in the current sample tray configuration the error message is generated Probable Causes Incorrect tray or trays installed Incorrect vial positions defined in the method or sequence Tray recognition defective dirty sample tray or defective transport assembly flex board Suggested Actions Install the correct trays or edit the method or sequence accordingly Ensure ...

Страница 84: ...d gripper assembly to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the ChemStation the ALS maintenance positions can be selected from the Maintenance menu in the Diagnosis display In the Control Module the functions can be selected in the Test screens of the autosampler ...

Страница 85: ...le and needle seat Change Piston relieves the tension on the metering spring draws the piston to the outer position enabling easy disassembly of the metering head assembly Park Arm Park Gripper secures the gripper arm to the park position behind the sampling unit ready for transport or shipping of the autosampler Home moves the tray arm to its home position for better access and exchange of the tr...

Страница 86: ...15 mm above the needle seat Needle Up Up Arrow Press function key couple of times to move the needle arm up in 2 mm steps Needle Down Down Arrow Press function key couple of times to move the needle arm down in 2 mm steps The lowest position end position is used to align the needle at the correct position in the needle seat End Done Completes the procedure by moving the gripper arm to the home pos...

Страница 87: ...dle arm to the maintenance position 3 Remove the front cover 4 Exchange the needle or needle seat see Needle Seat Assembly on page 114 and Needle Assembly on page 111 5 Replace the front cover 6 Select End Done to complete the procedure NOTE Do not remove the front cover until the needle arm is in its maintenance position Removing the cover while the needle arm is activated may lock up the system ...

Страница 88: ...e User Interface The commands for the ChemStation and Control Module different names for commands in the Control Module are shown in brackets are Start Change Draws the piston away from the home position relieving the tension on the spring End Done Repositions the plunger at the home position Using the Change Seal Function 1 Select Start Change to move the piston to the maintenance position 2 Exch...

Страница 89: ... that can be selected from the Maintenance menu in the Diagnosis display In the Control Module the Park Gripper command is located in the Control display of the autosampler The commands for the ChemStation and Control Module different names for commands in the Control Module are shown in brackets are Park Arm Park Gripper moves the gripper arm to the park position Home moves the gripper arm out of...

Страница 90: ...ntrol Module different names for commands in the Control Module are shown in brackets are Start Change Moves the transport assembly and gripper arm to the position required to change the gripper arm End Done Repositions the transport assembly and gripper arm to the home position Using the Change Seal Function 1 Select Start Change to move the gripper arm to the maintenance position 2 Exchange the ...

Страница 91: ...e Control Module the Align Tray function is located in the Control display of the autosampler Additional information can be found in the on line information systems The correct procedure for the Control Module are 1 Set alignment to factory default Go to Align Tray and press Default Go to Align Transport and press Default NOTE The alignment procedure requires an Agilent 1200 Series Control Module ...

Страница 92: ...8 Visually re evaluate if the vial is in the center of the gripper fingers and correct X and Theta position accordingly 9 Press Enter to hit Next Vial enter vial 95 and press Goto Vial 10 Repeat steps 4 to 8 to align the gripper at position 95 11 Press Average F8 to balance the alignment 12 Press Done F6 to store the alignment permanently in non volatile memory and to reset the module 13 To check ...

Страница 93: ...he ChemStation the step commands can be selected from the Test Selection Box in the Diagnosis display In the Control Module the step commands can be accessed from the pull down menu in the autosampler Test Table 11 Injector Step Commands Step Action Comments Bypass Switches injection valve to the bypass position Plunger Home Moves the plunger to the home position Needle Up Lifts the needle arm to ...

Страница 94: ...t of the vial Command also switches the valve to bypass if it is not already in that position Vial to Tray Returns the selected vial to the tray position Command also lifts the needle to the upper position Needle into Seat Lowers the needle arm into the seat Command also returns the vial to the tray position Mainpass Switches the injection valve to the mainpass position Needle Up Mainpass Lifts th...

Страница 95: ...tive injection valve Plunger Home Defective or dirty sensor on the sampling unit flex board Defective metering drive motor Needle Up Needle already in the upper position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Vial to Seat No vial in selected position Vial already in seat position Defective transport assembly motors Sticki...

Страница 96: ...it flex board Sticking needle arm assembly Defective needle drive motor Mainpass Valve already in mainpass Valve not connected Defective injection valve Needle Up Mainpass Blockage in the sample loop or needle no solvent flow Needle already in the upper position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Valve already in main...

Страница 97: ...fingers with error messages motor overtemp 0 or 1 or 2 or 3 movement failed 0 or 1 or 2 or 3 missing vial Many times the sampler is being used very heavily 2 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial with error messages motor overtemp 0 or 2 movement failed 0 or 2 3 Poor alignment seen during vial pickup and vial replacement and...

Страница 98: ...ee the service note G1313 017 3 Very heavy usage use a macro A pre sequence macro QMBUVHW_PDF will automatically reset the sampler at the start of a sequence ChemStation 4 Check if the INJECT line is used in the Injector Program Remove this line from the program In this mode the system does not need this command to do the injection A firmware revision 3 81 will address this problem For more detail...

Страница 99: ...de the motor X or theta on the holder bracket in the appropriate direction and test the tension with the torque2 macro Repeat this steps until the values are in the appropriate torque range 9 Exchange the sample transport assembly G1329 60009 10 Exchange the main board part number G1329 69520 Table 13 Typical ranges Theta both 30 50 X axis both 50 90 Z axis both 90 130 Gripper open 30 65 Gripper c...

Страница 100: ...ds X axis and clean them If the rod is dirty or sticky clean it with Isopropanol and wipe it with a lint free cloth The rod can be lubricated with the following synthetic oil part number 6040 0854 3 Lubricate the X gear Friction can result in the belt slipping on the gear so that the position of the belt teeth towards the gear changes To avoid this apply some grease from the sample transport repai...

Страница 101: ... to be loosened For this slide the motor X or theta on the holder bracket in the appropriate direction and test the tension with the Torque2 mac macro Repeat this steps until the values are in the appropriate torque range 6 Reset the sampler alignments to default value Reset tray alignment and transport alignment is possible with the Control Module and the Chemstation To reset the transport alignm...

Страница 102: ...e Reset tray alignment and transport alignment is possible with the Control Module and the Chemstation To reset the transport alignment with the Chemstation enter following command in the command line Print sendmodule lals tray alig 0 00 0 00 3 Lubricate the X gear Friction can result in the belt slipping on the gear so that the position of the belt teeth towards the gear changes To avoid this app...

Страница 103: ...lue is too high the belt needs to be loosened For this slide the motor on the holder bracket in the appropriate direction and test the tension with the Torque2 mac macro Repeat this steps until the values are in the appropriate torque range 7 Exchange the sample transport assembly part number G1329 60009 8 Exchange the main board part number G1329 69520 Table 15 Typical ranges Theta both 30 50 X a...

Страница 104: ...104 Agilent 1200 Series Autosampler Reference Manual 3 Troubleshooting and Test Functions ...

Страница 105: ...d and Preparative Autosamplers Reference Manual Agilent Technologies 4 Repairing the Autosampler Introduction into Repairing the Autosampler 106 Overview of Main Repair Procedures 109 Simple Repairs 110 Exchanging Internal Parts 132 ...

Страница 106: ...lap Flex Board It is strongly recommended that the exchange of the safety flap and flex board is done by Agilent trained service personnel Transport Assembly Parts The adjustment of the motors and the tension on the drive belts are important for correct operation of the transport assembly see Transport Assembly on page 139 It is strongly recommended that exchange of drive belts and the gripper ass...

Страница 107: ...a solution of water and a mild detergent Do not use an excessively damp cloth that liquid can drip into the autosampler Using the ESD Strap Electronic boards are sensitive to electronic discharge ESD In order to prevent damage always use an ESD strap supplied in the standard accessory kit when handling electronic boards and components 1 Unwrap the first two folds of the band and wrap the exposed a...

Страница 108: ...108 Agilent 1200 Series Autosampler Reference Manual 4 Repairing the Autosampler Figure 14 Using the ESD Strap ...

Страница 109: ...nual 109 Repairing the Autosampler 4 Overview of Main Repair Procedures Figure 15 Main Assemblies Needle page 111 Rotor seal page 120 Metering seal page 124 Needle seat page 114 Transport assembly page 139 ASM board page 157 Power supply page 169 ...

Страница 110: ...amage or blockage 15 minutes See Needle Assembly on page 111 Exchanging the seat assembly When the seat shows indication of damage or blockage 10 minutes See Needle Seat Assembly on page 114 Exchanging the rotor seal After approximately 30000 to 40000 injections or when the valve performance shows indication of leakage or wear 30 minutes See Rotor Seal on page 120 Exchanging the metering seal When...

Страница 111: ...87103 needle seat Needle assembly 900 µl loop cap G1313 87202 for G1313 87101 needle seat Needle assembly 900 µl loop cap G2260 87201 for G2260 87101 needle seat Preparations for this procedure Select Start in the maintenance function Change Needle see Change Needle on page 86 When the needle is positioned approx 15 mm above the needle seat remove the front cover WARNING To avoid personal injury k...

Страница 112: ...r 1 Select Needle Down until the needle screw is aligned with the hole in the safety cover 2 Remove the sample loop fitting from the needle fitting 3 Loosen the fixing screw and lift out the needle 4 Insert the new needle Align the needle in the seat then tighten the screw firmly ...

Страница 113: ...op fitting to the needle fitting 6 Use Needle Up to lift the needle to a position approximately 2mm above the seat 7 Ensure the needle is aligned with the seat On completion of this procedure Install the front cover Select End in the maintenance function Change Needle see Change Needle on page 86 CellFrame ...

Страница 114: ...7101 for G1329A Needle seat assy 0 12 mm i d 1 2 µl G1313 87103 for G1329A Needle seat assy 0 50 mm i d 20 µl G2260 87101 for G2260A Preparations for this procedure Select Start in the maintenance function Change Needle see Change Needle on page 86 Remove the front cover Use the Needle Up command in the Change Needle function to lift the needle an addition 1 cm 1 Disconnect the seat capillary fitt...

Страница 115: ...ressthe seat firmly into position 4 Connect the seat capillary fitting to port 5 of the injection valve 5 Use Down to position the needle approximately 2 mm above the seat 6 Ensure the needle is aligned with the seat If required bend the needle slightly until the needle is aligned correctly CellFrame CellFrame CellFrame ...

Страница 116: ...ent 1200 Series Autosampler Reference Manual 4 Repairing the Autosampler On completion of this procedure Install the front cover Select End in the maintenance function Change Needle see Change Needle on page 86 ...

Страница 117: ...n accessory kit Parts required Stator face 0100 1851 for G1329A Stator face 0101 1268 for G2260A Preparation Remove the front cover Remove the leak tubings if necessary CAUTION The stator face is held in place by the stator head When removing the stator head ensure the stator face does not fall out of the valve 1 Remove all capillary fittings from the injection valve ports 2 Loosen each fixing bol...

Страница 118: ...lace the stator face in place on the stator head Ensure the pins on the stator engage in the holes in the stator head 5 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure 6 Reconnect the pump capillaries to the valve ports see Hydraulic Connections on page 35 ...

Страница 119: ...Agilent 1200 Series Autosampler Reference Manual 119 Repairing the Autosampler 4 7 Slide the waste tube into the waste holder in the leak tray On completion of this procedure Install the front cover ...

Страница 120: ...tor seal 0100 1853 Vespel for G1329A Rotor seal 0100 1849 Tefzel for G1329A Rotor seal 0101 1268 PEEK for G2260A Preparations for this procedure Remove front cover Remove the leak tubing if necessary CAUTION The stator face is held in place by the stator head When removing the stator head ensure the stator face does not fall out of the valve 1 Remove all capillary fittings from the injection valve...

Страница 121: ...he rotor seal and isolation seal 5 Install the new rotor seal and isolation seal Ensure the metal spring inside the isolation seal faces towards the valve body 6 Install the stator ring with the short of the two pins facing towards you at the 12 O Clock position Ensure the ring sits flat on the valve body CellFrame CellFrame CellFrame CellFrame ...

Страница 122: ...ace in place on the stator head 8 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure 9 Reconnect the pump capillaries to the valve ports see Hydraulic Connections on page 35 10 Slide the waste tube into the waste holder in the leak tray ...

Страница 123: ...Agilent 1200 Series Autosampler Reference Manual 123 Repairing the Autosampler 4 On completion of this procedure Install the front cover ...

Страница 124: ...alytical head Metering seal 0905 1294 pack of 1 for 900 µl analytical head Metering plunger 5063 6586 for 100 µl analytical head Metering plunger 5062 8587 for 900 µl analytical head only if scratched or contaminated Preparations for this Procedure Select Start in the maintenance function Change piston see Change Piston on page 88 Remove the front cover 1 Remove the two capillaries from the meteri...

Страница 125: ...fixing bolts from the base of the metering head assembly 4 Disassemble the metering head assembly 5 Use a small screwdriver to carefully remove the seal Clean the chamber with lint free cloth Ensure all particular matter is removed 6 Install the new seal Press the seal firmly into position CellFrame ...

Страница 126: ... assembly Carefully insert the plunger into the base The closed side of the metering head must be on the same side as the lower one of the two capillary drillings 9 Install the fixing bolts Tighten the bolts securely 10 Install the metering head assembly in the autosampler Ensure the large hole in the metering head is facing downwards CellFrame CellFrame ...

Страница 127: ...osampler Reference Manual 127 Repairing the Autosampler 4 11 Reinstall the capillaries On completion of this procedure Install the front cover Select End in the maintenance function Change piston see Change Piston on page 88 ...

Страница 128: ... Preparations for this procedure Select Start in the maintenance function ChangeGripper see Change Gripper Change Arm on page 90 Turn off the power to the autosampler Remove the front cover 1 Identify the slit below the gripper motor and the gripper arm release button 2 Rotate the arm approximately 2 5cm 1 inch to the left and insert the straightened paper clip into the slit CellFrame slit button ...

Страница 129: ...l engage on an internal catch and the rotation of the arm will be blocked 4 Hold the paper clip in place press the gripper release button and rotate the gripper arm to the right 5 The gripper arm will come off 6 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left CellFrame CellFrame CellFrame CellFrame ...

Страница 130: ...130 Agilent 1200 Series Autosampler Reference Manual 4 Repairing the Autosampler On completion of this procedure Install the front cover Turn the power to the autosampler ON ...

Страница 131: ...e board from the autosampler 4 Install the interface board Secure the screws 5 Reconnect the cables to the board connectors Frequency At installation or when defective Tools required Flat head screwdriver Parts required Interface board see Optional Interface Boards on page 243 CAUTION The interface board is sensitive to electrostatic discharge Always use the ESD strap when handling electronic boar...

Страница 132: ...the front panel is turned off To disconnect the autosampler from line power unplug the power cord WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous sol...

Страница 133: ...the top cover on the bench 3 Press the side panels into the slots in the top cover Tools required None Parts required G1329 68713 Cover kit for G1329A G2260A 5042 8901 Name plate NOTE The plastics kit contains all parts but it is not assembled CAUTION Observe the assembly instructions carefully The main cover cannot be disassembled once assembled incorrectly Figure 17 Assembling the Main Cover Nam...

Страница 134: ...face board installed Flat head screwdriver Parts required Foam kit G1313 68702 CAUTION This procedure requires removal of the MIO interface board The board is sensitive to electrostatic discharge Always use the ESD strap when handling electronic boards 1 Switch OFF the autosampler at the main power switch Remove the power cable 2 If installed remove the ECB board ...

Страница 135: ... the Autosampler 4 3 Move the safety lever to the left Lift the clips on the top cover 4 Lift the cover away 5 Unscrew the three screws on the top plate Remove the top plate 6 Carefully remove the top foam CellFrame safety lever clip CellFrame CellFrame CellFrame ...

Страница 136: ...essing internal parts Tools required If interface board installed Flat head screwdriver Parts required None 1 Install the top foam Press the foam firmly into place 2 Engage the slot on the top plate onto the side plate 3 Fix the top plate in place with the three screws 4 Engage the top cover into the bottom cover CellFrame CellFrame ...

Страница 137: ...0 Series Autosampler Reference Manual 137 Repairing the Autosampler 4 5 Lower the cover into place Ensure the clips close firmly 6 Slide the power lock to the right and install the power cable CellFrame CellFrame ...

Страница 138: ...ore removing the transport assembly Tools required None Parts required Illumination assembly G1367 60040 1 The illumination assembly is located at the top rail of transport assembly After disconnecting the cable from the autosampler mainboard the illumination assembly can be removed by turning the rail approximately 60 degrees 2 Then lift the complete assembly To install a new illumination assembl...

Страница 139: ...y as described on page 138 3 Lift out the transport assembly This may require a flat head screwdriver to separate the transport assembly from the sampling unit Frequency Sticking or jammed transport assembly Defective flex board or sensors Tools required If interface board installed Flat head screwdriver Parts required Transport assembly G1329 60009 for G1329A G2260A Figure 18 Removing the Transpo...

Страница 140: ...port assembly into the autosampler 2 Ensure the slot on the top of the transport assembly engages with the stud on the sampling unit On completion of this procedure Ensure the transport assembly is seated firmly Install the top cover and foam page 134 Verify the transport assembly alignment page 102 Transport assembly Sampling unit ...

Страница 141: ...mpling unit G2260 60008 for G2260A The sampling units come without injection valve and analytical head assembly see Sampling Unit Assembly on page 178 Preparations for this procedure Remove the front cover Remove the vial tray Remove the top cover page 134 Remove the Illumination assembly page 138 Remove the transport assembly page 139 1 Remove the pump and column thermostat capillaries ports 1 an...

Страница 142: ... sampling unit and tray base back to disengage the sampling unit connector 4 Lift the sampling unit and tray base approximately 10 cm Slide the leak sensor out of the leak plane 5 Lift the sampling unit and tray base out of the autosampler 6 Turn the tray base locking screw turn anti clockwise ...

Страница 143: ...Agilent 1200 Series Autosampler Reference Manual 143 Repairing the Autosampler 4 7 Slide the tray base back to disengage the tray base from the sampling unit ...

Страница 144: ...ng the complete sampling unit remove the injection valve and metering head from the defective sampling unit Install the valve and metering head in the new sampling unit See Injection Valve Assembly on page 147 and Gripper Arm on page 128 1 Mount the tray base onto the sampling unit Ensure the tray base is positioned flush against the sampling unit 2 Tighten the locking screw turn clockwise Correct...

Страница 145: ...base slide the leak sensor into the holder in the leak plane 4 Place the sampling unit and tray base into the autosampler 5 Slide the sampling unit and tray base forwards Ensure the sampling unit connector is seated correctly 6 Install the pump and column thermostat capillaries ports 1 and 6 in the injection valve Connector ...

Страница 146: ... Repairing the Autosampler 7 Install the waste tube in the holder in the leak tray On completion of this procedure Install the transport assembly page 139 Install the top cover page 134 Install the vial tray Verify the transport assembly alignment page 102 ...

Страница 147: ...Parts required Injection valve 0101 0921 for G1329A Injection valve 0101 1267 for G2260A Preparations for this procedure Remove all capillaries from the injection valve page 35 Remove the top cover page 134 Remove the transport assembly page 139 Remove the sampling unit page 141 1 Disconnect the valve cable connector from the SUD board labelled Valve Assy 2 Remove the three fixing screws ...

Страница 148: ... 4 Repairing the Autosampler 3 Remove the injection valve assembly 4 Feed the cable of the new valve through to the SUD board 5 Connect the valve cable to the connector labelled Valve Assy 6 Fix the valve assembly in place securely with the three screws ...

Страница 149: ...iring the Autosampler 4 On completion of this procedure Install the sampling unit page 141 Install the transport assembly page 139 Install the top cover page 134 Replace the injection valve capillaries page 35 Verify the transport assembly alignment page 102 ...

Страница 150: ...d Pozidrive No 1 screwdriver Parts required Metering drive motor 5062 8590 Belt 1500 0697 Preparations for this procedure Remove the top cover page 134 Remove the transport assembly page 139 Remove the sampling unit page 141 1 Disconnect the motor connector from the SUD board labelled Metering M 2 Remove the four fixing screws 3 Remove the motor 4 Remove the belt ...

Страница 151: ... SUD board Connect the cable to labelled Metering M 7 Install the motor with the four fixing screws Ensure the belt is seated correctly over the gear and motor shaft On completion of this procedure Install the sampling unit page 141 Install the transport assembly page 139 Install the top cover page 134 Verify the transport assembly alignment page 102 ...

Страница 152: ...tor and Belt Frequency When defective Tools required Pozidrive No 1 screwdriver Parts required Needle drive motor 5062 8590 Belt 1500 0697 Preparations for this procedure Remove the top cover page 134 1 Lift away the drive belt 2 Disconnect the motor connector from the SUD board labelled Needle M ...

Страница 153: ...er 4 3 Remove the metering head assembly see Gripper Arm on page 128 4 Remove the four fixing screws and remove the motor 5 Feed the motor cable through to the SUD board Connect the cable to the connector labelled Needle M 6 Secure the motor in place with the four fixing screws ...

Страница 154: ...Reference Manual 4 Repairing the Autosampler 7 Install the belt 8 Installthemetering headassembly see Gripper Arm on page 128 On completion of this procedure Install the top cover page 134 Verify the transport assembly alignment page 102 ...

Страница 155: ...ations for this procedure Remove the top cover and foam page 134 Remove the transport assembly page 139 CAUTION The ASM board is sensitive to electrostatic discharge Always use the ESD strap see Using the ESD Strap on page 107 when handling electronic boards 1 Disconnect the fan connector from the ASM board 2 Lift the fan and carefully pull the fan out of the foam ...

Страница 156: ...er 3 Install the new fan Ensure the cable is installed as shown 4 Connect the fan connector to the ASM board On completion of this procedure Install the transport assembly page 139 Install the top cover and foam page 134 Verify the transport assembly alignment page 102 CellFrame ...

Страница 157: ...p cover and foam page 134 Remove the transport assembly page 139 CAUTION The ASM board is sensitive to electrostatic discharge Always use the ESD strap see Using the ESD Strap on page 107 when handling electronic boards NOTE This procedure requires reloading the autosampler firmware reprogramming of the instrument serial number and realignment of the gripper 1 Remove the connector screws from the ...

Страница 158: ...e GPIB and RS232 connector screws On completion of this procedure On the new board check the switch setting of address switch S1 see Table 50 on page 252 or Table 51 on page 253 Note An incorrect switch setting e g TEST BOOT may cause the autosampler to turn in a basic mode yellow or red flashing status light In such a case turn off the pump re set the address switches and turn on the pump again C...

Страница 159: ... number The serial number can be entered using either the control module or the ChemStation see Entering the Serial Number using the Control Module on page 163 or see Entering the Serial Number using the ChemStation on page 165 8 Check the firmware revision of the autosampler If the firmware revision is older than the current firmware revision of the autosampler update the firmware see Replacing t...

Страница 160: ... specific commands on the command line at the bottom of the main user interface screen 1 To enter a module serial number type the following command into the command line print sendmodule lals ser YYYYYYYYYY Where YYYYYYYYYY is the 10 character serial number of the module in question The reply line will respond with RA 0000 SER followed by the module serial number you just entered To change the typ...

Страница 161: ...he up down arrows select the module where you have to change the product number or serial number 3 Press PN SN This will display a screen where you can enter the product number and or serial number 4 Make your changes using the information from the product label of your module 5 Press OK to highlight the complete command 6 Press Done to transfer the information into the main board s memory Press C...

Страница 162: ...play the correct type for this module Using the Control Module G1323B 1 Connect the control module to the detector Turn ON the detector 2 On the control module press System F5 then Records F4 Using the up down arrows make sure that the detector is highlighted 3 Press FW Update F5 then m This will display a box which says Update Enter Serial 4 Press Enter This will display the box labeled Serial 5 ...

Страница 163: ...y Below the box a reply line should then say Reply RA 0000 TYPE XXXXX XXXXX is what you just entered 6 Turn the module off then on again Turn on should be normal In the Records screen the product column should indicate the module you just entered If an Agilent ChemStation is also connected re boot it now Entering the Serial Number using the Control Module 1 Connect the control module to the autosa...

Страница 164: ...autosampler off then on again The Records screen should display the correct serial number for this module 7 If a ChemStation is also connected re boot the ChemStation now as well NOTE For firmware revisions below A02 00 it is very important never to press Done if the Serial box is blank In this case the module can no longer be recognized by either the control module or the ChemStation The main boa...

Страница 165: ... the module serial number you just entered 2 Turn OFF the autosampler then on again Then re boot the ChemStation If the serial number you have just entered is different than the original module serial number you will be given the opportunity to edit the configure Agilent 1200 Series access screen during the re boot of the ChemStation 3 After boot up the serial number you have just entered can be s...

Страница 166: ...w firmware is required if new version solves problems of currently installed version if after exchange of the mainboard ASM the version on board is older than previous installed one To upgrade the autosampler firmware the following steps have to be performed Load the firmware into the autosampler see the help system of your user interface ...

Страница 167: ...arts required SUD board G1313 66503 Preparations for this procedure Remove the top cover page 134 Remove the transport assembly page 139 Remove the sampling unit page 141 CAUTION Take care not to damage the flex board when removing the SUD board 1 Disconnect all connectors from the SUD board 2 Remove the two fixing screws and lift out the board Fixing screws ...

Страница 168: ... Secure the board in place with the two screws 5 Reconnect all the connectors to the new board On completion of this procedure Install the sampling unit page 141 Install the transport assembly page 139 Install the top cover page 134 Verify the transport assembly alignment page 102 Fixing screws Leak sensor Metering drive Injection Valve Needle drive Flex board ...

Страница 169: ...cedure Remove the top cover page 134 Remove the transport assembly page 139 Remove the sampling unit page 141 Remove the ASM board page 157 Remove the fan page 155 CAUTION The ASM board is sensitive to electrostatic discharge Always use the ESD strap see Using the ESD Strap on page 107 when handling electronic boards 1 Remove the bottom foam 2 Remove the power switch light pipe from the coupler Ce...

Страница 170: ...m the rear panel 4 Lift the power supply out of the module 5 Remove the coupler from the of the power supply 6 Insert the coupler onto the switch of the new power supply 7 Install the power supply in the module 8 Connect the light pipe to the coupler CellFrame CellFrame CellFrame CellFrame CellFrame CellFrame ...

Страница 171: ...erence Manual 171 Repairing the Autosampler 4 9 Install the bottom foam Ensure the cables are positioned as shown On completion of this procedure Install the ASM board page 157 Install the fan page 155 Install the sampling unit page 141 ...

Страница 172: ...s required Leak sensor 5061 3356 Preparations for this procedure Remove the top cover page 134 Remove the transport assembly page 139 Remove the sampling unit page 141 1 Disconnect the leak sensor from the SUD board labelled leak sensor Remove the sensor 2 Feed the cable of the new sensor through the base of the sampling unit to the SUD board ...

Страница 173: ...he Autosampler 4 3 Connect the connector to the connector labelled leak sensor On completion of this procedure Install the sampling unit page 141 Install the transport assembly page 139 Install the top cover page 134 Verify the transport assembly alignment page 102 ...

Страница 174: ...174 Agilent 1200 Series Autosampler Reference Manual 4 Repairing the Autosampler ...

Страница 175: ... Injection Valve Assembly 184 Sheet Metal Kit 186 Cover Parts 187 Foam Parts 188 Power and Status Light Pipes 189 Leak System Parts 190 Vial Trays 191 Standard Autosampler Accessory Kit G1329 68725 192 Preparative Autosampler Accessory Kit G2260 68705 193 Maintenance Kit G1313 68709 194 Multi Draw Kit G1313 68711 195 900 µl Injection Upgrade Kit G1363A for G1329A 196 External Tray G1313 60004 197 ...

Страница 176: ...176 Agilent 1200 Series Autosampler Reference Manual 5 Parts and Materials Main Assemblies Figure 19 Autosampler Main Assemblies 5 4 2 1 10 9 8 6 3 7 ...

Страница 177: ...sembly for G1329A Injection valve assembly for G2260A 0101 0921 0101 1267 5 Vial tray thermostatted see page 191 G1329 60011 6 Gripper assembly G1313 60010 7 Illumination assembly G1367 60040 8 Autosampler Main Board ASM for G1329A 1389A 2260A G1329 69500 Standoff GPIB connector part not shown 0380 0643 Standoff remote connector part not shown 1251 7788 9 Ribbon cable sample transport G1313 81601 ...

Страница 178: ...02 G1313 87303 5022 2133 5 Analytical head assembly 100 µl for G1329A Preparative head assembly 900 µl for G1329A P 200Bar Preparative head assembly 900 µl for G2260A P 400Bar 01078 60003 G1313 60007 G2260 60007 6 Inj valve Anal head cap 160 mm 0 25 mm for G1329A Inj valve Prep head cap 160 mm 0 50 mm for G2260A G1313 87301 G2258 87301 7 Injection valve assembly for G1329A Injection valve assembly...

Страница 179: ...29 87101 or G1329 87103 needle seat Needle assembly 900 µl loop capillary for G1313 87101 needle seat Needle assembly 900 µl loop capillary for G2260 87101 needle seat G1313 87201 G1329 80001 G1313 87202 G2260 87201 Clamp Kit includes needle clamp and 2 x clamp screw G1313 68713 Table 18 Autosampler Sampling Unit Assembly continued Item Description Part Number ...

Страница 180: ...180 Agilent 1200 Series Autosampler Reference Manual 5 Parts and Materials Figure 20 Autosampler Sampling Unit Assembly 1 2 15 14 13 12 11 8 7 10 6 4 3 9 5 ...

Страница 181: ...ssembly 100 µl for G1329A Item Description Part Number Analytical head assembly includes items 1 6 01078 60003 1 Screws 0515 0850 2 Plunger assembly 5063 6586 3 Adapter 01078 23202 4 Support seal assembly 5001 3739 5 Metering seal pack of 2 5063 6589 6 Head body 01078 27710 Screw M5 60 mm lg for mounting of assembly 0515 2118 ...

Страница 182: ...182 Agilent 1200 Series Autosampler Reference Manual 5 Parts and Materials Figure 21 Analytical Head Assembly 2 3 4 5 6 1 ...

Страница 183: ...05 1294 6 Head body 900 µl G1313 27700 Screw M5 60 mm lg for mounting of assembly 0515 2118 Table 21 Preparative Head Assembly 900 µl for G2260A Item Description Part Number Preparative head assembly 900 µl includes items 1 6 This head is limited to 400 Bars It can only be assembled on a sampling unit with the description supports 900 µl at 400 Bar G2260 60007 1 Plunger assembly 900 µl 5062 8587 2...

Страница 184: ...embly Table 22 Injection Valve Assembly for G1329A Item Description Part Number 1 Injection valve assembly includes items1 6 0101 0921 2 Isolation seal 0100 1852 3 Rotor seal Vespel 0100 1853 3 Rotor seal Tefzel 0100 1849 4 Stator face 0100 1851 5 Stator head 0100 1850 6 Stator screws 1535 4857 ...

Страница 185: ...for G2260A Item Description Part Number 1 MBB Injection valve assembly includes items1 6 MBB Make Before Brake is a trademark by Rheodyne 0101 1267 2 Isolation seal 0100 1852 3 4 Rotor seal PEEK Stator face PEEK kit 0101 1268 5 Stator head 0100 2195 6 Stator screws 1535 4857 Figure 22 Injection Valve Assembly 1 2 6 3 5 4 ...

Страница 186: ...erence Manual 5 Parts and Materials Sheet Metal Kit Table 24 Sheet Metal Item Description Part Number 1 Slot cover 5001 3772 2 Screw cover 5022 2112 3 Autosampler Sheet metal kit for G1329A G2260A G1329 68701 Figure 23 Sheet Metal Kit 1 2 3 ...

Страница 187: ...ame plate for Agilent 1200 Series 5042 8901 3 Transparent front cover G1313 68714 4 Door repair kit includes transparent side and front door G1329 68707 5 Light protection kit includes opaque side and front door opaque front cover G1329 68708 Cabinet upgrade kit includes side panels top cover transparent side and front door front cover and side insulation cover for cooled autosampler G1329 68706 F...

Страница 188: ...nce Manual 5 Parts and Materials Foam Parts Table 26 Foam Parts Item Description Part Number Foam kit includes items 2 and 3 G1313 68702 1 Board guides 5041 8395 2 Top foam Order foam kit 3 Bottom foam Order foam kit Figure 25 Foam Parts 2 3 1 ...

Страница 189: ...ower and Status Light Pipes Table 27 Power and Status Light Pipes Item Description Part Number 1 Power switch button 5041 8381 2 Light pipe power switch 5041 8382 3 Power switch coupler 5041 8383 4 Light pipe status lamp 5041 8384 Figure 26 Power and Status Light Pipes 1 2 3 4 ...

Страница 190: ...rials Leak System Parts Table 28 Leak System Parts Item Description Part Number 1 Leak funnel holder 5041 8389 2 Leak sensor 5061 3356 3 Leak plane G1313 44511 4 Leak tubing 120 mm reorder gives 5 m 5062 2463 5 Leak funnel 5041 8388 Figure 27 Leak System Parts 1 5 4 3 2 ...

Страница 191: ...Number 1 Adapter air channel G1329 43200 2 Tray for 100 2 ml vials thermostattable G1329 60011 3 Spring G1313 09101 4 Tray base for G1329A G2260A includes items 4 5 G1329 60000 5 Spring stud 0570 1574 Halftray for 40 2 ml vials not shown G1313 44512 Halftray for 15 6 ml vials not shown G1313 44513 Figure 28 Thermostatted Autosampler Vial Trays and Tray Base 4 2 1 3 5 ...

Страница 192: ...7 Label halftray no PN Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel adapter G132...

Страница 193: ...PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel adapter G1329 43200 Tray for 15 x 6 ml vials x2 G1313 44513 U...

Страница 194: ... and Materials Maintenance Kit G1313 68709 Table 32 Maintenance Kit Item Description Part Number 1 Rotor seal Vespel 0100 1853 2 Needle assembly G1313 87201 3 Needle seat assembly G1313 87101 4 Metering seal pack of 2 5063 6589 5 Finger caps pack of 15 5063 6506 ...

Страница 195: ...195 Parts and Materials 5 Multi Draw Kit G1313 68711 Table 33 Multi Draw Kit Item Description Part Number 1 Seat capillary 500 µl 0 5 mm id G1313 87307 2 Seat capillary 1500 µl 0 9 mm id G1313 87308 2 Seat capillary 5000 µl 0101 0301 3 Union 5022 6515 ...

Страница 196: ... and Materials 900 µl Injection Upgrade Kit G1363A for G1329A Table 34 900 µl Injection Upgrade Kit Item Description Part Number 1 Analytical Head 900 µl G1313 60007 2 Loop Extension 900 µl G1313 87303 3 Union loop extension 5022 2133 4 Needle 900 µl G1313 87202 ...

Страница 197: ...lent 1200 Series Autosampler Reference Manual 197 Parts and Materials 5 External Tray G1313 60004 Table 35 External Tray Item Description Part Number 1 External tray G1313 60004 2 Disposal tube G1313 27302 ...

Страница 198: ...198 Agilent 1200 Series Autosampler Reference Manual 5 Parts and Materials ...

Страница 199: ...erence Manual Agilent Technologies 6 Identifying Cables Cable Overview 200 Analog Cables 202 Remote Cables 205 BCD Cables 210 Auxiliary Cable 212 CAN Cable 213 External Contact Cable 214 RS 232 Cable Kit 215 LAN Cables 216 This chapter provides information on cables ...

Страница 200: ...grators 01040 60101 3394 6 integrators 35900 60750 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remote cables 3390 integrator 01046 60203 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series I integrator 03394 60600 3396 Series II 3395A integrator see page 207 3396 Series III 3395B integrator 03396 61010 Agilent 1200 1050 modules 1046A FLD 5061 3378 10...

Страница 201: ... contacts Agilent 1200 Series interface board to general purpose G1103 61611 GPIB cable Agilent 1200 module to Agilent ChemStation 1 m 10833A Agilent 1200 module to Agilent ChemStation 2 m 10833B RS 232 cable Agilent 1200 module to a computer This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter 34398A LAN cable Cross over network cable shielded 3 m long for poi...

Страница 202: ... connected to Agilent 1200 Series modules The other end depends on the instrument to which connection is being made Agilent 1200 to 3390 2 3 Integrators Connector 01040 60101 Pin 3390 2 3 Pin Agilent 1200 Signal Name 1 Shield Ground 2 Not connected 3 Center Signal 4 Connected to pin 6 5 Shield Analog 6 Connected to pin 4 7 Key 8 Not connected ...

Страница 203: ... Agilent 1200 to 3394 6 Integrators Agilent 1200 to BNC Connector Connector 35900 60750 Pin 3394 6 Pin Agilent 1200 Signal Name 1 Not connected 2 Shield Analog 3 Center Analog Connector 8120 1840 Pin BNC Pin Agilent 1200 Signal Name Shield Shield Analog Center Center Analog ...

Страница 204: ...gilent 1200 Series Autosampler Reference Manual 6 Identifying Cables Agilent 1200 to General Purpose Connector 01046 60105 Pin 3394 6 Pin Agilent 1200 Signal Name 1 Not connected 2 Black Analog 3 Red Analog ...

Страница 205: ... to Agilent 1200 Series modules The other end depends on the instrument to be connected to Agilent 1200 to 3390 Integrators Connector 01046 60203 Pin 3390 Pin Agilent 1200 Signal Name Active TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start req...

Страница 206: ...1 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low Connector 01046 60210 Pin 3394 Pin Agilent 1200 Signal Name Active TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High 6 ...

Страница 207: ...rwise the integrator prints START not ready NOTE START and STOP are connected via diodes to pin 3 of the 3394 connector Connector 03394 60600 Pin 3394 Pin Agilent 1200 Signal Name Active TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start reques...

Страница 208: ...w 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Connector 5061 3378 Pin HP 1050 Pin Agilent 1200 Signal Name Active TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 ...

Страница 209: ...und NC 2 Brown Prepare run Low 4 3 Gray Start Low 7 4 Blue Shut down Low 8 5 Pink Not connected NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low Connector 01046 60201 Pin Universal Pin Agilent 1200 Signal Name Active TTL 1 White Digital ground 2 Brown Prepare run Low 3 Gray Start Low 4 Blue Shut down Low 5 Pink Not connected 6 Yellow Power on High 7 Red ...

Страница 210: ...rument to be connected to Agilent 1200 to General Purpose Connector G1351 81600 Wire Color Pin Agilent 1200 Signal Name BCD Digit Green 1 BCD 5 20 Violet 2 BCD 7 80 Blue 3 BCD 6 40 Yellow 4 BCD 4 10 Black 5 BCD 0 1 Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 not connected ...

Страница 211: ...dentifying Cables 6 Agilent 1200 to 3396 Integrators Connector 03396 60560 Pin 3392 3 Pin Agilent 1200 Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD0 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5 V Low ...

Страница 212: ...e provides a modular plug to be connected to the Agilent 1200 Series vacuum degasser The other end is for general purpose Agilent 1200 Series Degasser to general purposes Connector G1322 61600 Color Pin Agilent 1200 Signal Name White 1 Ground Brown 2 Pressure signal Green 3 Yellow 4 Grey 5 DC 5 V IN Pink 6 Vent ...

Страница 213: ...les 6 CAN Cable Both ends of this cable provide a modular plug to be connected to Agilent 1200 Series module s CAN bus connectors Agilent 1200 module to module 0 5 m 5181 1516 Agilent 1200 module to module 1 m 5181 1519 Agilent 1200 module to control module G1323 81600 ...

Страница 214: ...al purpose Agilent 1200 Series Interface Board to general purposes 5 10 15 1 11 6 Connector G1103 61611 Color Pin Agilent 1200 Signal Name White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connec...

Страница 215: ...r cable and one adapter Use the cable and adapter to connect Aligent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1200 module to PC RS 232 Cable Kit 34398As Instrument DCD RX TX DTR GND DSR RTS CTS RI DCD RX TX DTR GND DSR RTS CTS RI PC 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 DB9 Male DB9 Female DB9 Female DB9 Male ...

Страница 216: ...Manual 6 Identifying Cables LAN Cables Recommended Cables Table 37 Description Part number Cross over network cable shielded 3 m long for point to point connection 5023 0203 Twisted pair network cable shielded 7 m long for hub connections 5023 0202 ...

Страница 217: ...ve Autosamplers Reference Manual Agilent Technologies 7 Introduction to the Autosampler Introduction to the Autosampler 218 Sampling Sequence 220 Sampling Unit 223 Transport Assembly 226 Early Maintenance Feedback EMF 228 Electrical Connections 230 ...

Страница 218: ... rack holding 100 1 8 ml vials is available for use with the thermostatted autosamplers The half size racks trays are not designed for an optimal heat transfer when they are used with a thermostatted autosampler The autosamplers transport mechanism uses an X Z Theta movement to optimize vial pick up and return Vials are picked up by the gripper arm and positioned below the sampling unit The grippe...

Страница 219: ...the preparative autosampler are available Control of the vial temperature in the thermostatted autosampler is achieved using an additional Agilent 1200 Series module the ALS thermostat Details of this module are given in the Agilent 1200 Series thermostatted autosampler Supplemental Manual Figure 29 Overview of the Autosampler Switching Valve Needle seat Transport assembly ASM board Power supply S...

Страница 220: ...valve returns to the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 2 The plunger of the metering device moves to the initialization position 3 The gripper arm moves from the home position and selects the vial At the same time the needle lifts out of the seat 4 The gripper arm places th...

Страница 221: ...s position the mobile phase flows through the autosamplers metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over When the sample sequence begins the valve unit switches to the bypass position Figure 31 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Figure 30 Mainp...

Страница 222: ...ws the sample into the sample loop Figure 32 When the metering unit has drawn the required volume of sample into the sample loop the needle is raised and the vial is replaced in the sample tray The needle is lowered into the needle seat and the injection valve switches back to the mainpass position flushing the sample onto the column Figure 33 Figure 32 Drawing the Sample Figure 33 Mainpass Positi...

Страница 223: ...s three main assemblies needle drive metering device and injection valve The sampling units for the standard and the preparative autosamplers are different NOTE The replacement sampling unit excludes the injection valve and metering head assemblies Figure 34 Autosampler Sampling Unit Metering device Injection valve Needle drive ...

Страница 224: ...ent of the spindle to linear motion The drive nut pushes the sapphire plunger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampl...

Страница 225: ...ers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass end positions No valve adjustments are required after replacing internal components Table 39 Injection Valve Technical Data Standard Preparative MBB Motor type 4V 1 2A stepper motor 4V 1 2A stepper motor Seal material Vespel Tefzel available PEEK Stator material Ceramic...

Страница 226: ... drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly The stepper motor positions are determined by the optical encoders mounted onto the stepper motor housing The enc...

Страница 227: ...ialization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly ...

Страница 228: ...etable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The autosamplers provides two EMF counters Each counter increments with autosamplers use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero af...

Страница 229: ...ific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary make note of the values displayed by the injection valve and needle movements counters Enter these values or values slightly less than the displayed v...

Страница 230: ...ever use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Figure 36 Autosampler plus Thermostat Electrical Connections GPIB RS232C CAN bus Remote Vial number output Relay contacts Connection ALS Thermostat G1330B Thermostat optional ...

Страница 231: ...d by the configuration switch module next to the GPIB connector The software needs the appropriate drivers to support this communication See your software documentation for further information The Thermostat Autosampler connection is used for control signal transfer and synchronization of the two modules The cable must be installed for operation of the ALS thermostat The power input socket accepts...

Страница 232: ...232 Agilent 1200 Series Autosampler Reference Manual 7 Introduction to the Autosampler ...

Страница 233: ... Technologies 8 Theory of Operation Autosampler Control and Electronics 234 Position and Movement Sensors 235 Autosampler Main Board ASM 236 Firmware Description 241 Optional Interface Boards 243 Interfaces 247 Setting the 8 bit Configuration Switch 252 The Main Power Supply Assembly 257 ...

Страница 234: ...The ASM board controls the vial transport mechanism sampling needle metering unit and high speed injection valve These devices are controlled by a versatile electronics design based upon a 68000 family processor which also contains battery backup RAM flash ROM a real time clock and several communications options ...

Страница 235: ...ple Transport Flex Board Sensor Type Number of Sensors Position Movement Sensed Reflection Sensor 6 Vial tray identification Reflection Sensor 1 Gripper initialization Reflection Sensor 3 Transport assembly Initialization Table 41 Sampling Unit Flex Board Sensor Type Number of Sensors Position Movement Sensed IR light sensor 1 Metering device home reference position Reflection sensor 2 Needle end ...

Страница 236: ...ess for the GPIB communication baud rate for RS 232 transfer and so on Also the ASIC controls and drives module specific functions and reads static status signals Leak Converter Solvent leaking from the autosampler cools down the PTC This changes the resistance of the PTC causing the leak converter to generate a leak signal The leak converter consists of a PTC for leak sensing and an NTC for ambie...

Страница 237: ...or diagnostics Electronic Fuses The circuits that are connected to 36 V are fused on the board electronically Onboard Battery An onboard lithium battery buffers the electronic memories when the module is turned off For safety information on lithium batteries see Lithium Batteries Information on page 267 Autosampler Specific Electronics The autosampler specific functions provided by the electronics...

Страница 238: ...e up down signal are used in the FPGA to provide instantaneous stepper motor commutation with respect to the motor rotor position Wiring between the autosampler main board ASM and the motors and encoders uses a flat band cable 64 pin and a flex board on which 10 reflection light sensors are located Six light sensors are used for vial tray identification one for gripper decoding and three for decod...

Страница 239: ...alve motor is done by two microswitches Two reflection light sensors are used to detect the end positions of the needle arm One photo sensor is required to detect the home position of the metering device Two hall sensors detect correct closure of the door needle arm movement is interrupted if the door is open All the sensors are mounted on one flex board The flex board and motors are connected to ...

Страница 240: ...agram FPGA theta 1 Z Axis X Axis Sampling Unit Metering Device Injection Valve Core common Resources DSP theta 2 Limit switch signals 8 bit data 5 bit address Encoder feedback data Quadrature decoder Serial data Encoder clocks and direction FPGA chip select 5 bit address 8 bit data TPU signals I O signals ...

Страница 241: ...e the complete communication capabilities GPIB CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities GPIB CAN LAN and RS 232C memory management ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common funct...

Страница 242: ...e file naming conventions are xxxx vvv DLB where xxxx is the product number e g 1329 for the G1329A Autosampler and vvv is the revision number for example 380 is revision 3 80 For instructions refer to your user interface NOTE Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Figure 40 Firmware Update Mechanism Resident ...

Страница 243: ...tosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused Table 43 Optional Interface Boards Description Part Number BCD Board G1351 68701 Fuse 250 mA four are on the board 2110 0004 LAN Communication Interface Board G1369A or G1369 60001 Figure 41 Block Diagram BCD Board Processor interface Board identification B...

Страница 244: ...t see BCD Cables on page 210 and the external outputs see External Contact Cable on page 214 to external devices Table 44 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD 5 20 2 BCD 7 80 3 BCD 6 40 4 BCD 4 10 5 BCD 0 1 6 BCD 3 8 7 BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 12 BCD 9 200 13 BCD 8 100 15 5V Low ...

Страница 245: ...314A G1310A G1311A G1312A and G1329A or newer and on all other Agilent 1200 Series modules a DOS ChemStation software revision A 06 01 or above Table 45 LAN Boards Type Vendor Supported networks G1369A G1369 60001 Agilent Technologies Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX recommended for re ordering J4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T J4105A Hewlett Packard Token Ring 8...

Страница 246: ...utosampler Reference Manual 8 Theory of Operation Recommended Cables Cross over network cable shielded 3 m long for point to point connection 5023 0203 Twisted pair network cable shielded 7 m long for hub connections 5023 0202 ...

Страница 247: ...cation of the connectors Figure 6 on page 28 Table 46 Agilent 1200 Series Interfaces Interface Type Pumps Autosampler DA Detector MW Detector FL Detector VW Detector RI Detector Thermostatted Column Compartment Vacuum Degasser CAN Yes Yes Yes Yes Yes No GPIB Yes Yes Yes Yes Yes No RS 232C Yes Yes Yes Yes Yes No Remote Yes Yes Yes Yes Yes Yes Analog Yes No 2 1 No Yes Interface board Yes Yes Yes Yes...

Страница 248: ...t address and recognized by the operating software from Agilent Technologies CAN Interface The CAN is an intermodule communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement Remote Interface The APG remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features su...

Страница 249: ...and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level is defined as standard TTL levels 0 V is logic true 5 V is false fan out is 10 input load is 2 2 kOhm against 5 V and outputs are open collector type inputs outputs wired or technique Table 48 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPA...

Страница 250: ...9 pin male SUB D type connector The pins are defined as follows 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Table 48 Remote Signal Distribut...

Страница 251: ...mpler Reference Manual 251 Theory of Operation 8 Figure 42 RS 232 Cable Instrument DCD RX TX DTR GND DSR RTS CTS RI DCD RX TX DTR GND DSR RTS CTS RI PC 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 DB9 Male DB9 Female DB9 Female DB9 Male ...

Страница 252: ...s provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures Figure 43 8 bit Configuration Switch Table 50 8 bit Configuration Switch Mode Select 1 2 3 4 5 6 7 8 GPIB 0 0 GPIB Address RS 232C 0 1 Baudrate Data Bits Parity Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC factory setting is shown for autosampler 1 8 1 0 ...

Страница 253: ...l remain in the non volatile memory In this way you can store more than one set of parameters using the same 8 bit configuration switch twice for example for both GPIB and RS 232C GPIB Default Addresses If you just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses whe...

Страница 254: ...the values in the non volatile memory Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Table 52 Communication Settings for RS 232C Communication Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baud rate Data Bits Parity Table 53 Baud Rate Settings Switches Baud Rate Switches Baud ...

Страница 255: ...ng both up will allow for normal operation If you use the following switch settings and power the instrument up again a forced cold start has been completed To return to normal operation set switches back to your GPIB or RS 232 configuration settings Table 55 Parity Settings Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity CAUTION Forced cold start erases all methods and data store...

Страница 256: ...ther positions other than being both up will allow for normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a detector It only uses basic functions of the operating system for example for communication To return to normal operation set switches back to your GPIB or RS 232C config...

Страница 257: ...rs The line voltage can vary in a range from 100 120 or 220 240 volts AC 10 and needs no manual setting To disconnect the instrument from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off Figure 44 Main Power Supply MPS Blockdiagram 3 6 V s u p p l y 2 4 V s u p p l y 5 V s w i t c h i n g r e g u l a t o r l i n e a r v o l...

Страница 258: ...rn off output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument off wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Table 58 Main Power Supply Spec...

Страница 259: ...259 Agilent 1200 Series Standard and Preparative Autosamplers Reference Manual Agilent Technologies 9 Specifications Performance Specifications 260 ...

Страница 260: ... safe leak handling low voltages in maintenance areas error detection and display Injection range 0 1 100 µl in 0 1 µl increments Up to 1500 µl with multiple draw hardware modification required Replicate injections 1 99 from one vial Precision 0 25 RSD from 5 100 µl 1 RSD 1 5 µl variable volume Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial or 1 µl from 10 µl sample in 300 µl micr...

Страница 261: ...low voltages in maintenance areas error detection and display Injection range 0 1 900 µl in 0 1 µl increments recommended 1 µl increments Up to 1800 µl with multiple draw hardware modification required Replicate injections 1 99 from one vial Precision Typically 0 5 RSD of peak areas from 5 2000 µl Typically 1 RSD of peak areas from 2000 5000 µl Typically 3 RSD of peak areas from 1 5 µl Minimum sam...

Страница 262: ...maintenance areas error detection and display Injection range 0 1 900 µl in 0 1 µl increments recommended 1 µl increments Up to 1800 µl with multiple draw hardware modification required Up to 5000 µl with multiple draw hardware modification required Replicate injections 1 99 from one vial Precision Typically 0 5 RSD of peak areas from 5 2000 µl Typically 1 RSD of peak areas from 2000 5000 µl Typic...

Страница 263: ...ative Autosamplers Reference Manual Agilent Technologies A Safety Information General Safety Information 264 Lithium Batteries Information 267 Radio Interference 268 Sound Emission 269 Solvent Information 270 Agilent Technologies on Internet 272 ...

Страница 264: ...tory instrument for research and routine application only It is not certified for in vitro or medical applications Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected t...

Страница 265: ...ny unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Safety Symbols Table 62 shows safety symbols used on the instrument and in ...

Страница 266: ...ductor terminal Eye damage may result from directly viewing the light produced by the Xenon flash lamp used in this product Always turn the xenon flash lamp off before removing it WARNING A warning alerts you to situations that could cause physical injury or damage to the equipment Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution al...

Страница 267: ... ICAO ADR RID IMDG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries WARNING Lithiumbatteri Eksplosionsfare ved fejlagtig håndtering Udskiftning må kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandøren WARNING Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun b...

Страница 268: ...echnologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises ...

Страница 269: ...ation This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 dB Sound Pressure Lp 70 dB A At Operator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test ...

Страница 270: ...all of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like nitri...

Страница 271: ...ers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF ...

Страница 272: ...Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1200 Series modules for download ...

Страница 273: ...ls and caps 50 cleaning the autosampler 107 common electronics 236 condensation 16 configuration switch 252 D damaged packaging 18 delay 42 delay volume 39 42 delivery checklist 18 DRAW 50 draw speed 50 E EJECT 50 eject speed 50 electronics 234 ASIC 236 ASM board 236 battery 237 electronic fuses 237 fan drive 237 firmware 241 sample transport control 238 sampling unit control 239 sensors 235 envir...

Страница 274: ...31 preparation 29 safety 26 29 tray cover and front cover 39 installing the tray cover front door 39 instrument status indicator 56 interface board BCD LAN 243 interfaces analog signal output 248 APG remote 248 CAN 248 GPIB 248 overview 247 RS 232C 250 internet 272 introduction to the autosampler 218 IR sensor 235 L LAN cables 216 interface board 243 LAN interface board 245 low volume injections 5...

Страница 275: ...ampler accessory kit contents 20 preparative head 224 R reflection sensor 235 repair procedures 109 repairs ASM board 157 exchanging internal parts 132 fan 155 injection valve assembly 147 leak sensor 172 main board ASM 157 main cover 133 metering plunger 124 metering seal 124 metering drive motor and belt 150 needle assembly 111 needle drive motor and belt 152 needle seat assembly 114 power suppl...

Страница 276: ...er Reference Manual Index V valve capillaries 34 verifying the gripper position 93 vial contents temperature 260 vial numbering 36 vial racks 218 vial tray 39 vials 218 viscous samples 50 volume 42 W weight 15 X X axis 226 Z Z axis 226 ...

Страница 277: ......

Страница 278: ...echnical reference information about the Agilent 1200 Series Standard and Preparative Autosamplers The manual describes the following installing the autosamplers optimizing performance troubleshooting and test functions repairing the autosamplers parts and materials introduction to the autosamplers screens of the local control module specifications safety and warranty ...

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