22
Anoxomat
Mark II CTS
User’s guide
Chapter 3
Optional functions
3.3 Pre-programmed recipes
Pre-programmed recipe for oxygen level, gas mixture
and evacuation level
Rather than accept the standard microaerophilic recipe
with a resulting oxygen level of 6%, you may wish to
incubate using a different recipe (i.e., Helicobacter
species with 5% oxygen). Oxygen levels can be set
between 0 and approximately 18%.
If you purchased secondary gas supplies for your
Anoxomat, these gas supplies need to be programmed
according to your specifications. Oxygen levels can be
demanded between 0 and 18%. CO2 and/or H2 levels can
be chosen within certain limitations, depending upon the
required O2 level and the installed gas mixtures.
Maximum evacuation level in the standard program is a
default setting:
+/- 205 mbar for the anaerobic recipe and +/- 300 mbar
for the microaerophilic. If this level of vacuum may be
harmful to cell culture or the agar, recipes can be
programmed with the following maximum evacuation
levels: 400, 500 or 600 mbar.
You can order one or more recipes according to your
specification from Advanced Instruments or your local
supplier. These pre-programmed recipes are saved in the
Anoxomat for future reference. (see Activating pre-
programmed or user-defined recipes).
3.4 Additional jar connections
If you need to be able to process more than one
jar at a time, you can order one or more additional jar
connections (including software update). The Anoxomat
allows a maximum of 4 jar connections (jars and gases).
3.5 Additional gas connections
If your type of culture or research requires more than
one gas supply, you can order one or more additional
gas connections (including software update). The
Anoxomat allows a maximum of three gas connections,
and a total maximum of five connections (jars and
gases). Refer to the Standard Gas Mixture section in
Chapter 1 for additional information.
Secondary gas supplies
For creating non-standard atmospheres, other gas
mixtures can be connected if more gas connections
are available.
Gas
Connection
Cylinder Contents
Usage
Primary
N
2
Nitrogen
85%
Standard
anaerobic and
microaerophilic
recipe
CO
2
Carbon
Dioxide
10%
H
2
Hydrogen 5%
Secondary 1
CO
2
Carbon
Dioxide
100%
Increased levels
of CO2
Gas
Connection
Cylinder Contents
Usage
Primary
N
2
Nitrogen
85%
Standard
anaerobic and
microaerophilic
recipe
CO
2
Carbon
Dioxide
10%
H
2
Hydrogen 5%
Secondary 1
CO
2
Carbon
Dioxide
100%
Increased levels
of CO2
Secondary 2
H
2
Hydrogen 14%
(max) Variable levels
of H2
N
2
Nitrogen
86%
Table 3.1: Primary and one secondary gas supply
Table 3.2: Primary and two secondary gas supplies
