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LSM 510
QUICKSTART
LSM 510 META
Quick Start in the Expert Mode
Carl Zeiss
B 45-0008 e 10/02
4-21
•
To further improve image quality you can slow
down the scan speed, allowing more photons
to integrate on the detector, or apply image
averaging to remove random noise, or a
combination of both. These adjustments are
made by clicking on the
Mode
button on the
Scan Control
window.
•
Set the
Scan Speed
in the
Speed
panel.
•
Select the
Line
or
Frame
average
Mode
, the
Mean
or
Sum
average
Method
and the
Number
of averages in the
Depth, Scan
Direction & Scan Average
panel accordingly
by observing your image. The setting average of
16
(
Number 16
) should improve signal / noise
dramatically; however, the image acquisition
rate will be slower.
•
When the image optimization has been
finished, click on the
Single
button to generate
a single image of the specimen.
•
If your specimen is sensitive to photobleaching,
you can attenuate the laser illumination by
clicking on the
Channels
button in the
Scan
Control
window. At the bottom of the window
you can set the percentage of laser power
(
Transmission [%]
) for each excitation
wavelength. You will probably have to increase
the
Detector Gain
if you decrease the laser
power. This setting controls the transmission
degree of the Acousto-Optical Tunable Filters
(AOTF).
Try to use as low laser light intensity as possible to prevent sample bleaching. For that purpose
increase the detector gain to a value of approximately 800 V.
Fig. 4-20
Scan Control window
Summary of Contents for LSM 510 META
Page 1: ...LSM 510 and LSM 510 META Laser Scanning Microscopes Operating Manual Release 3 2...
Page 8: ...INTRODUCTION LSM 510 Carl Zeiss LSM 510 META VIII B 45 0008 e 10 02...
Page 10: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss Contents LSM 510 META 1 2 B 45 0008 e 10 02...
Page 20: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss LSM 510 META 1 12 B 45 0008 e 10 02...
Page 22: ...LSM 510 SETUP REQUIREMENTS LSM 510 Carl Zeiss Contents LSM 510 META 2 2 B 45 0008 e 10 02...
Page 52: ...QUICKSTART LSM 510 Carl Zeiss Contents LSM 510 META 4 2 B 45 0008 e 10 02...