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LSM 510
QUICKSTART
LSM 510 META
Quick start in the Expert Mode
Carl Zeiss
B 45-0008 e 10/02
4-13
4.3
Quick Start in the Expert Mode
Proceed as follows to generate images in the Expert Mode:
−
start the Expert Mode
−
test / change the microscope setting: objective, fluorescence / attenuation filters, illumination mode,
diaphragms
−
normal setting of the microscope on the specimen with observation in brightfield or fluorescence
contrast (KÖHLER-type illumination)
−
switch to
LSM
mode
−
setting of lasers: laser type, laser intensity (power)
−
configuration of beam path and channel assignment: tracks, multi-tracks, switching on / off of laser
lines and intensity (excitation)
−
image creation: determine the scan method (line, frame) and scan parameters (image size, scan
speed, pixel depth, scan direction, scan average, zoom, rotation, offset)
−
image optimization and storage
4.3.1
Start the Expert Mode
•
Double-click on the
LSM 510
icon on the
WINDOWS NT operating system desktop.
−
The
LSM 510 Switchboard
menu appears
on the screen.
•
Click on the
Scan New Images
button and
Start Expert Mode
button in the
LSM 510
Switchboard
menu.
−
The LSM will go through the initialization
and open the
Main
menu labeled
LSM 510
Expert Mode
. The
Main
menu appears on
the screen.
Fig. 4-10
LSM 510 Switchboard menu
Fig. 4-11
Main menu of LSM 510 - Expert Mode
Summary of Contents for LSM 510 META
Page 1: ...LSM 510 and LSM 510 META Laser Scanning Microscopes Operating Manual Release 3 2...
Page 8: ...INTRODUCTION LSM 510 Carl Zeiss LSM 510 META VIII B 45 0008 e 10 02...
Page 10: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss Contents LSM 510 META 1 2 B 45 0008 e 10 02...
Page 20: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss LSM 510 META 1 12 B 45 0008 e 10 02...
Page 22: ...LSM 510 SETUP REQUIREMENTS LSM 510 Carl Zeiss Contents LSM 510 META 2 2 B 45 0008 e 10 02...
Page 52: ...QUICKSTART LSM 510 Carl Zeiss Contents LSM 510 META 4 2 B 45 0008 e 10 02...