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QUICKSTART
LSM 510
Carl Zeiss
Quick Start in the Expert Mode
LSM 510 META
4-20
B 45-0008 e 10/02
Proceed as follows for image optimization:
•
Click on the
Channels
button in the
Scan
Control
window.
−
The
Channel Settings
panel and the
Excitation of Track (...)
panel are displayed
in the
Scan Control
window.
•
Press the
Cont.
(continuous scan) button on
the right-hand side of the window. This starts
the continuous image acquisition, which can be
interrupted by pressing the
Stop
button.
•
Under the
Channel Settings
panel all buttons
for each channel you have set up are displayed.
Click
Ch1
(channel 1 of track 1), for example, if
you want to adjust the first image displayed in
the split mode window.
•
Use the
Pinhole
slider to set the pinhole
diameter.
−
The selected pinhole diameter should be
small enough to still allow the Detector Gain
setting and to provide sufficient image
information. 1 Airy unit is a decent value to
obtain a confocal XY-image (use the
1
button).
•
If required, adjust the pinhole again (see
Main
menu,
Maintain
subordinate toolbar,
Pinhole
button).
•
Use the
Detector Gain
slider to set image contrast and brightness. This adjustment is very sensitive.
Try using the left and right arrows to make the adjustment instead of dragging the slider bar. Use the
Shift
and
Crtl
keys for changing to coarse and fine steps.
•
To adjust the black level (background) use
Ampl. Offset
.
•
Also, try adjusting the microscope by manual focusing. Sometimes you will find that there are other
focal planes within the specimen which are brighter, and therefore the detector gain will need to be
turned down.
•
Once you have optimized a particular channel, you can switch to the next channel required and
repeat the optimization.
•
As soon as all channels are optimized, click on the
Stop
button.
Fig. 4-19
Scan Control window
Summary of Contents for LSM 510 META
Page 1: ...LSM 510 and LSM 510 META Laser Scanning Microscopes Operating Manual Release 3 2...
Page 8: ...INTRODUCTION LSM 510 Carl Zeiss LSM 510 META VIII B 45 0008 e 10 02...
Page 10: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss Contents LSM 510 META 1 2 B 45 0008 e 10 02...
Page 20: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss LSM 510 META 1 12 B 45 0008 e 10 02...
Page 22: ...LSM 510 SETUP REQUIREMENTS LSM 510 Carl Zeiss Contents LSM 510 META 2 2 B 45 0008 e 10 02...
Page 52: ...QUICKSTART LSM 510 Carl Zeiss Contents LSM 510 META 4 2 B 45 0008 e 10 02...