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Carl Zeiss
Axiolab A
3-8
B 40-015 e 06/99
(3)
Transmitted-Light Polarization - Determination of the n
γγγγ
'
Vibration Direction and
Determination of the Path Difference
Fig. 3-6
Determine the n
γγγγ
'
vibration direction using the example of an artificial fiber
Application
The position of the two directions with the relatively highest (n
γ
'
) and the relatively lowest (n
α
'
) refractive
index, or the absolutely highest (n
γ
) and the absolutely lowest (n
α
) refractive index, in relation to
morphological directions of crystal surfaces, crystal needles or fibers, is an important criterion for
recognition. It is also used, for example, for the diagnosis of biocrystals (gout, pseudogout).
Settings
•
Set the microscope as usual for transmitted-
light brightfield examinations.
•
Turn stage until the first stop position, unlock
stop and turn the stage until the object features
maximum darkness.
•
Activate stage stop mechanism and turn stage
around 45° until the next stop so that the
longitudinal axis of the fiber is oriented in NE-
SW direction.
•
Allocate a path difference of approx. 150 nm to
this color on the MICHEL LEVY color chart.
−
When the
λ
-compensator (3-5/
2
) has been
pushed in, the color of the fiber changes to
yellow-orange (path difference approx. 400
nm).
−
When the stage is turned around 90°, the
fiber appears greenish blue (path difference
approx. 700 nm).
150
700
-400
λ
γ
λ
γ
γ
γ
γ
Fig. 3-7
Schematic diagram of the color
chart in addition and subtraction
position
Summary of Contents for Axiolab A
Page 1: ...Axiolab A Reflected Light Microscope Operating Manual...
Page 16: ...Carl Zeiss Axiolab A 1 6 06 99 B 40 015 e Fig 1 3 Axiolab A microscope configurations...
Page 18: ...Carl Zeiss Axiolab A 1 8 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 1 of 2...
Page 20: ...Carl Zeiss Axiolab A 1 10 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 2 of 2...
Page 26: ...Carl Zeiss Axiolab A 1 16 06 99 B 40 015 e Fig 1 5 Axiolab A function elements 1 of 2...
Page 27: ...Axiolab A Carl Zeiss B 40 015 e 06 99 1 17 Fig 1 5 Axiolab A function elements 2 of 2 1 1 2...
Page 34: ...Carl Zeiss Axiolab A 1 24 06 99 B 40 015 e...
Page 36: ...Carl Zeiss Axiolab A 2 2 B 40 015 e 06 99...
Page 44: ...Carl Zeiss Axiolab A 3 2 B 40 015 e 06 99...
Page 68: ...Carl Zeiss Axiolab A 4 2 B 40 015 e 06 99...