Carl Zeiss
Axiolab A
3-4
B 40-015 e 06/99
3.2
Illumination and Contrasting Techniques
The description of the illumination and contrasting techniques is based on the following microscope
settings:
•
The Axiolab A microscope is ready for operation as described in chapter 2 and switched on as
described in section 3.1.
•
The pushrod (3-2/
3
) has been pushed in to direct 100% of the light to the observation port of the
binocular tube.
3.2.1
Setting of Reflected-Light Brightfield
•
Place a specimen of as much contrast as
possible (3-2/
6
) on the specimen stage (3-2/
10
);
if required by the specimen height, lower the
specimen stage via the coarse drive (3-2/
9
) or
secure it in the lower stop position.
•
Pull out plane glass pushrod (3-2/
2
).
•
Look through the binocular tube (3-2/
1
) and set
the eyepiece distance and interpupillary
distance again as required (also see section 2.4).
•
The aperture diaphragm (3-2/
4
) must be set to
approx. half the size of the opening diameter
by turning the adjustment wheel downwards.
•
Pull out pushrod for luminous-field diaphragm
(3-2/
5
) until stop.
•
Use the coarse/fine drive (3-2/
9, 8
) focus the
circular luminous-field diaphragm now visible in
the field of view.
•
Insert the pushrod for the luminous-field
diaphragm (3-2/
5
) again; the entire field of view
is now visible.
•
Refocusing on the specimen can be performed via the fine drive (3-2/
8
).
•
Use the illuminance control (3-2/
7
) to set the brightness in the field of view to a value agreeable to
your eyes.
☞
Depending on the specimen, the contrast is now adjusted via the aperture diaphragm (3-2/
4
).
However, the aperture diaphragm cannot be used to control the image brightness (loss in
image resolution).
1
1
2
3
4
5
6
7
8
9
10
Fig. 3-2
Setting of reflected-light brightfield
Summary of Contents for Axiolab A
Page 1: ...Axiolab A Reflected Light Microscope Operating Manual...
Page 16: ...Carl Zeiss Axiolab A 1 6 06 99 B 40 015 e Fig 1 3 Axiolab A microscope configurations...
Page 18: ...Carl Zeiss Axiolab A 1 8 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 1 of 2...
Page 20: ...Carl Zeiss Axiolab A 1 10 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 2 of 2...
Page 26: ...Carl Zeiss Axiolab A 1 16 06 99 B 40 015 e Fig 1 5 Axiolab A function elements 1 of 2...
Page 27: ...Axiolab A Carl Zeiss B 40 015 e 06 99 1 17 Fig 1 5 Axiolab A function elements 2 of 2 1 1 2...
Page 34: ...Carl Zeiss Axiolab A 1 24 06 99 B 40 015 e...
Page 36: ...Carl Zeiss Axiolab A 2 2 B 40 015 e 06 99...
Page 44: ...Carl Zeiss Axiolab A 3 2 B 40 015 e 06 99...
Page 68: ...Carl Zeiss Axiolab A 4 2 B 40 015 e 06 99...