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Carl Zeiss
Axiolab A
1-18
06/99 B 40-015 e
1.5
Objectives
The objectives are the optical centerpiece of the microscope. The following is an example of how
objectives can be labelled:
Epiplan 10
×
/0.20
∞
/-
where
10
×
:
objective magnification, with a defined color ring on the objective being allocated to each
magnification step (Carl Zeiss color ring code)
0.20
:
numerical aperture
∞
:
infinite tube length
-
:
can be used with cover slip thickness D = 0 or 0.17 mm
or
0
:
can only be used without cover slip, that means D = 0
0.17
:
can be used with cover slip thickness D = 0.17 mm
and
Oil
:
oil immersion objective
Ph 2
:
phase contrast objective with a green color ring and phase stop Ph 2
Color ring code for objective magnification
Color ring on
objective
black
brown
red
orange
yellow
green
light
blue
dark
blue
white
Magnification
factor
1.25
×
2.5
×
4
×
; 5
×
6.3
×
10
×
16
×
;20
×
;
25
×
;32
×
40
×
; 50
×
63
×
100
×
;
150
×
The objective magnification multiplied with the eyepiece magnification (usually 10
×
) results in the visual
overall magnification: e.g. 10
×
10 = 100
×
.
The numerical aperture
×
1000, e.g. 0.20
×
1000 = 200
×
, is the highest useful magnification, i.e. no
further details are resolved above that limit.
In transmitted-light applications, the exact observance of the cover slip thickness of 0.17 mm is all the
more necessary the higher the numeric aperture of the objective. Therefore, so-called "Korr" objectives
can be set for different cover slip thicknesses via a correction ring. For this, a specimen area is searched,
and the position of the correction ring where optimum focus and image contrast are obtained is
determined (refocusing is always required).
Immersion objectives are always insensitive to differences in cover slip thickness.
When immersion objectives are used, the air between the cover slip and the objective is replaced with a
liquid, which is immersion oil in most cases. The plastic oiler containing 20 ml of 581 N immersion oil is
particularly suitable for this purpose.
Summary of Contents for Axiolab A
Page 1: ...Axiolab A Reflected Light Microscope Operating Manual...
Page 16: ...Carl Zeiss Axiolab A 1 6 06 99 B 40 015 e Fig 1 3 Axiolab A microscope configurations...
Page 18: ...Carl Zeiss Axiolab A 1 8 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 1 of 2...
Page 20: ...Carl Zeiss Axiolab A 1 10 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 2 of 2...
Page 26: ...Carl Zeiss Axiolab A 1 16 06 99 B 40 015 e Fig 1 5 Axiolab A function elements 1 of 2...
Page 27: ...Axiolab A Carl Zeiss B 40 015 e 06 99 1 17 Fig 1 5 Axiolab A function elements 2 of 2 1 1 2...
Page 34: ...Carl Zeiss Axiolab A 1 24 06 99 B 40 015 e...
Page 36: ...Carl Zeiss Axiolab A 2 2 B 40 015 e 06 99...
Page 44: ...Carl Zeiss Axiolab A 3 2 B 40 015 e 06 99...
Page 68: ...Carl Zeiss Axiolab A 4 2 B 40 015 e 06 99...