YMC Pilot PLUS Manual Download Page 1

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Original-

Manual

Low pressure chromatography column

YMC Pilot

PLUS

In order to avoid personal injuries and damage to equipment, please read this manual carefully and

act on the recommendations given. Keep this manual accessible for the user at all times.

Version 1.3

26.03.2018

Summary of Contents for Pilot PLUS

Page 1: ...ography column YMC PilotPLUS In order to avoid personal injuries and damage to equipment please read this manual carefully and act on the recommendations given Keep this manual accessible for the user...

Page 2: ...YMC Europe GmbH Sch ttmannshof 19 46539 Dinslaken Germany 49 02064 427 0 Info ymc de www ymc de YMC Europe GmbH YMC Pilot 2 49 PLUS low pressure chromatography column...

Page 3: ...livery contents 15 4 2 Delivery inspection 15 5 Requirements for Operation 16 5 1 Pilot column set up 16 5 2 Pressure test 16 6 Operation 18 6 1 Removal of adjustable end piece 18 6 2 Pilot column pac...

Page 4: ...S low pressure chromatography column 10 Disposal 45 11 Appendix 46 11 1 Spare parts column stand 46 11 2 Spare parts adjustable end piece 46 11 3 Spare parts column body 70 100 mm ID 47 11 4 Spare par...

Page 5: ...signal word fields Always read the full text of the warning in order to effectively protect yourself from damage The following signal word fields marked by different colours and signal words distinct...

Page 6: ...ct of the action step to check whether the step has been performed correctly 2 Another action step Figures within instructions A figure is only related to the action step which is listed directly belo...

Page 7: ...atmospheric pressure at the intended maximum temperature 40 C Every use which is outside these specifications is defined as not intended use The intended use includes the compliance with the advice a...

Page 8: ...mmended personnel protective equipment The following protective equipment is required Symbol PPE Phase of life Action Protective gloves Operation Column packing Column unpacking Initiation Cleaning Sa...

Page 9: ...EU Guideline 2014 68 EU PED safety requirement Article 4 3 of EU Guideline 2014 68 EU Column body Borosilicate glass 3 3 Calibrated precision glass Duran KPG Frits PE Seals 75 Fluoroprene XP41 blue Co...

Page 10: ...n Good Good Limited Unstable Good Unstable 0 1M EDTA 3 Good Good Good Good Good Good 1M Acetic acid 6 Good Good Good Good Good Good Ethanol Good Good Good Good Good Good Ethyl acetate Good Good Unstab...

Page 11: ...Overview A parts list with all wetted parts and non wetted parts is in the appendix The YMC PilotPLUS column consists of the adjustable end piece the column body and the column stand These parts can b...

Page 12: ...ece consists of a closing disc and the piston The piston consists of the spindle the connecting flange the plunger the frit and the profile gasket A tubing goes through the spindle to the connecting f...

Page 13: ...glass body is fixed between the column disc and the closing disc by the rods The glass body has a visible scale Excessive solvent is removed during piston insertion via the connector drainage tubing T...

Page 14: ...CB 2 Plunger CB 5 Ring gasket A 3 Ring gasket B 3 4 3 Column stand 1 Levelling feet 3 Frame disc 2 Main pillar 4 Locking bolts With the locking bolts in the frame disc the column body is attached to...

Page 15: ...ion so that the column can always be shipped safely Delivery inspection Delivery inspection is performed as follows 1 Check the delivery for visible transport damage 2 Check the delivery for the compl...

Page 16: ...nnectors Perform the pressure test behind a safety screen During work on the pilot column wear the prescribed personnel protective equipment WARNING Pilot column under high pressure Eye or skin injuri...

Page 17: ...ith isopropanol 4 Increase the testing pressure in the pilot column by slow and stepwise adjustment of the flow rate at the pump The testing pressure is the pressure limit of the column plus 10 70 mm...

Page 18: ...fe operation the pilot column must be used for the intended use only Chapter 2 1 S 7 6 1 Removal of adjustable end piece Required devices Clean and soft surface The adjustable end piece is removed as...

Page 19: ...imum volume of the column The removal of fines decreases the column back pressure and so improves the performance of the packed column The slurry is prepared as follows 1 Mix the solvent or buffer wit...

Page 20: ...is trapped under or is inside the frit 7 Close the valve in the tubing on the column body 8 Connect the pump with the adjustable end piece 9 Flush the piston of the adjustable end piece with slurry s...

Page 21: ...OWN direction The adjustable end piece is ready for insertion into the column body 16 Put the piston of the adjustable end piece in a smooth angle opposite the drain function on the column body 17 Bri...

Page 22: ...ntinuously in a diagonal pattern to each other 20 Open the valve in the tubing on the adjustable end piece 21 Turn the piston of the adjustable end piece in the DOWN direction until the tubing is comp...

Page 23: ...Check and mark the bed length frequently 27 If the final bed length is reached stop the pump 28 Turn the piston of the adjustable end piece in the DOWN direction until the column bed is reached 29 Sta...

Page 24: ...the pump to the adjustable end piece 10 Flush the piston of the adjustable end piece with slurry solvent until the frit in the adjustable end piece is wetted completely 11 Disconnect the pump from th...

Page 25: ...OWN direction The adjustable end piece is ready for insertion into the column body 19 Put the piston of the adjustable end piece in a smooth angle opposite the drain function on the column body 20 Bri...

Page 26: ...on until the column bed is reached 25 Connect the pump to the tubing of the adjustable end piece 26 Open the valve in the tubing at the column body 27 Start the flow top down 28 Increase the flow rate...

Page 27: ...3 35 If no dead volume occurs the pilot column is released Chapter 6 3 S 27 36 Remove liquid above the connecting flange through the two holes in the adjustable end piece YMC recommends flushing this...

Page 28: ...ough contact with packing material Wear the prescribed personnel protective equipment The column is unpacked under flow as follows 1 Close the valve in the tubing of the column body 2 Disconnect the p...

Page 29: ...umn body clean the column body Chapter 7 3 S 33 12 If material remains in the column body put the column body into the stand with the open end facing down 13 Turn the column body slowly in the frame d...

Page 30: ...perating pressure Only authorised and qualified personnel should carry out the maintenance WARNING Incorrect replacement of spare parts Severe injuries due to breakage possible if unauthorised spare p...

Page 31: ...on the contamination three different procedures can be used 1 Regeneration 2 Sterilisation 3 Depyrogenation Regeneration Regeneration removes inorganic and organic contamination which binds to the ch...

Page 32: ...olysaccharides and other lipophilic substances Very good Good 7 2 Cleaning and maintenance plan The specified maintenance plan here is to be regarded as a general recommendation Depending on the frequ...

Page 33: ...ipment CAREFUL Packing material Eye and skin injuries possible through contact with packing material Wear the prescribed personnel protective equipment Required devices Wetted and lint free rags Colle...

Page 34: ...g agent and a wetted lint free rag 4 Put a collecting vessel below the column stand 5 Loosen the locking bolts and lock them by turning slightly 6 Lift the column body vertical from the column stand u...

Page 35: ...y 14 Loosen the locking bolts and lock them by turning slightly 15 Lift the column body vertical from the column stand using two holding bars which are vertically above each other 16 Insert the column...

Page 36: ...n with 140 mm and 200 mm ID are not exceeded Assemble the cylinder screws in a diagonal pattern and always tighten on the opposite sites Take care during the assembly of the glass body that all parts...

Page 37: ...lumn with 140 mm and 200 mm ID are not exceeded Assemble the cylinder screws in a diagonal pattern and always tighten on the opposite sites Take care during assembly of the glass body that affected pa...

Page 38: ...re chromatography column 5 Remove flat gasket 6 Remove glass body carefully 7 Position the new glass body on the ring gasket A 8 Check that the glass body is concentric with the gaskets 9 Put the flat...

Page 39: ...that the glass body is concentric with the column disc 13 ATTENTION Damage to the glass body is possible due to faulty assembly Note the maximum torque during the assembly of the glass body Insert the...

Page 40: ...not exceeded Assemble the cylinder screws in a diagonal pattern and always tighten on the opposite sites Take care during assembly of the glass body that affected parts are correctly aligned Required...

Page 41: ...Remove the ring gasket A 6 Remove the frit 7 Insert the frit The distribution profile faces the plunger 8 Reattach the ring gasket A and press firmly in position 9 Replace the fixed end piece into the...

Page 42: ...he eluted substances deteriorates Dead volume at the column inlet See fault description Dead volume occurred Partially clogged inlet frit Frit replacement Chapter 7 4 1 S 36 Partially clogged outlet f...

Page 43: ...f solvent leaks between glass body and flat gasket 2 Check the contact surface of the glass body and the sealing surface for impurities 3 Check the torque and the concentric arrangement of the parts T...

Page 44: ...hould be stored as follows unpacked cleaned dry empty 9 2 Transport We recommend the use the original wooden box for transportation If you are no longer in possession of the box please pack the column...

Page 45: ...sal YMC Pilot 45 49 PLUS low pressure chromatography column 10 Disposal For the disposal of the pilot column it has to be disassembled and disposed according to the national and other applicable envir...

Page 46: ...lon PTFE 11 2 Spare parts adjustable end piece Pos ID mm Part No Description Material 1 70 000014 Profile gasket 75 Fluoroprene XP41 2 70 000022 Frit AEP Poroplast UHMW PE 3 100 000114 Profile gasket...

Page 47: ...tubing Silicone 4 70 000024 Ring gasket A 75 Fluoroprene XP41 5 70 000023 Frit CB Poroplast UHMW PE 6 70 000015 Ring gasket B PTFE USP 7 70 850 000057 Glass body Borosilicate glass 3 3 8 100 000106 F...

Page 48: ...75 Fluoroprene XP41 4 140 000143 Frit CB Poroplast UHMW PE 5 140 000162 Ring gasket B PTFE USP 6 140 SI20 0LM24 0 Drainage tubing Silicone 7 140 850 000148 Glass body Borosilicate glass 3 3 8 200 0002...

Page 49: ...table end piece 36 column body 40 G Glass body replacement 37 P Packaging 45 Packing 19 Presonnel protective equipment 8 Pressure test 16 Protective equipment 8 R Resistance chemical 10 S Signal word...

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