GelDoc-It
TS3
and ChemiDoc-It
TS3
Imagers
33
81-0360-01 Rev B
image(s) to determine the intensity of the overlay
image(s) in the final composited image. Note that the
percentage shown pertains to the saturation levels in
the overlay image(s). Therefore, as higher
percentages are selected, only pixels with higher
saturation levels will be shown in the final
composited image. For example, if 65% is selected,
only pixels with over 65% saturation in the overlay
image will be shown in the final composited image.
Once the ideal composited image has been created,
press the
Checkmark
button below the Preview
composited image to complete fluorescent
compositing.
Merge Compositing Tool
Merge Compositing: The
Merge Compositing Tool
is used to merge up to three different images, pulling
a specific color channel (red, green or blue) from
each image. This tool is useful for multiplexing
applications.
The
Merge Compositing Tool
consists of three gray
rectangles where images can be placed (each
rectangle states “Drag image here”). The color
channel to be pulled from the image in each box is
shown in the colored bar below the gray box (red on
top, green in the middle, and blue on the bottom).
To use this tool, first open two or three images in the
gray area (gallery bar) below the “Compositing” tab.
Depending upon the desired color to be composited,
drag an image from the gallery bar to the “Drag
image here” area.
Each image’s histogram, pseudocolor and inversion
attributes can be adjusted to create the ideal
composited image. To do so, select the desired
image to be adjusted by pressing it. A black outline
will surround the selected image. Then, use the
Histogram
,
Pseudocolor
and
Invert
tools on the left
side of the screen to modify the image(s) and the
Preview composited image as desired.
Once the ideal composited image has been created,
press the
Checkmark
button below the Preview
composited image to complete fluorescent
compositing.
Chemiluminescent
Compositing Tool
Chemiluminescent Compositing: The
Chemiluminescent Compositing Tool
is used to
merge various image channels for chemiluminescent
imaging.
This tool functions exactly the same as the
Fluorescent Compositing Tool
described earlier,
except that the middle overlay image will always
automatically be inverted. This is ideal for
