Section 8- Protein A280
Lysozyme reference. Unknown (sample)
protein concentrations are calculated using the
mass extinction coefficient of 26.4 at 280 nm
for a 1% (10 mg/ml) Lysozyme solution.
User-entered values for molar extinction
coefficient (M
-1
cm
-1
) and molecular weight
(MW) in kilo Daltons for their respective
protein reference. Maximum value for e is
9999 X 1000 and maximum value for M.W. is
99999 X 1000.
User-entered mass extinction coefficient (L
gm
-1
cm
-1
) for a 10 mg/ml (1%) solution of the
respective reference protein.
λ
and Abs:
current value of the user-selectable wavelength cursor
and corresponding absorbance. The wavelength can be set by
dragging the cursor, using the up/down arrows or typing in the desired
wavelength. Note: The user-selected wavelength and absorbance
are not utilized in any calculations.
A280 10-mm Path:
10 mm-equivalent absorbance at 280 nm for the
protein sample measured
A260/280:
ratio of sample absorbance at 260 and 280 nm
Spectrum Normalization
The baseline is automatically set to the absorbance value of the
sample at 340 nm, which should be very nearly zero absorbance. All
spectra are referenced off of this zero. All data are now normalized
and archived in the same format. The user may elect to turn off the
baseline normalization, which may result in the spectra being offset
from the baseline.
8-3
