Section 8- Protein A280
Sample
Type
Detection
Limit
Approx.
Upper
Limit
Typical Reproducibility
(minimum 5 replicates)
(SD= mg/ml; CV= %)
Purified
BSA
0.10
mg/ml
100 mg/ml
sample range 0.10-10 mg/ml:
±
0.10 mg/ml
sample range >10mg/ml:
±
2%
Unique Screen Features
Sample Type:
There are six sample types (options) available for
purified protein analysis and concentration measurement. All of the
options can be viewed by clicking the mouse while it is positioned
within the ‘Sample Type’ box. The sample type (color-keyed) can be
selected by clicking on the preferred option or by scrolling through the
selections using the up or down arrow keys located to the left of the
sample type box. A description of each sample type is given below.
A general reference setting based on a 0.1%
(1 mg/ml) protein solution producing an
Absorbance at 280 nm of 1.0 A (where the
pathlength is 10 mm or 1 cm).
Bovine Serum Albumin reference. Unknown
(sample) protein concentrations are calculated
using the mass extinction coefficient of 6.7 at
280 nm for a 1% (10 mg/ml) BSA solution.
IgG reference. Unknown (sample) protein
concentrations are calculated using the mass
extinction coefficient of 13.7 at 280 nm for a
1% (10 mg/ml) IgG solution.
8-2
