Section 6- MicroArray
Unique Screen Features
Max Absorbance:
used to rescale the upper limit of the vertical axis.
Sample Type:
used to select the (color-keyed) type of nucleic acid
being measured. The user can select ‘DNA-50’ for dsDNA, ‘RNA-40’
for RNA, ssDNA-33’ for single-stranded DNA, or ‘Other’ for other
nucleic acids. The default is ssDNA-33’. If ‘other’ is selected, the
user can select an analysis constant between 15-150. When
navigating amongst the three (3) general sample types within the
Micro Array module, the last value of the constant entered within the
‘Constant’ Sample Type will be retained. See “Concentration
Calculation (Beer’s Law)” in the appendix for more details on this
calculation.
λ
and
Abs Norm:
the user selected wavelength (black cursor) and
corresponding absorbance at the 1mm pathlength. The wavelength
can be selected by dragging the black cursor or using the up/down
arrows in the wavelength box. Note: The user-selected wavelength
and absorbance at the 1 mm pathlength are not utilized in any
calculations.
Dye 1 (or 2):
user selected dye
Abs. Norm:
normalized absorbance of selected Dye at the 1 mm
pathlength.
pmol/ul:
concentration based upon selected Dye’s extinction
coefficient. See “Concentration Calculation (Beer’s Law)” in the
appendix for more details on this calculation.
ng/ul:
concentration of nucleic acids in the sample calculated using
the absorbance at 260 nm minus the absorbance at 340 nm (i.e.
6-3
