Section 5- Nucleic Acids
5. Nucleic Acids
Nucleic acid samples can be readily checked for concentration and
quality using the NanoDrop 1000 Spectrophotometer. To measure
nucleic acid samples select the ‘Nucleic Acid’ application module.
Sample Volume Requirements
Field experience has indicated that 1ul samples are sufficient to
ensure accurate and reproducible results when measuring aqueous
nucleic acid samples. However, if you are unsure about your sample
or your pipettor accuracy, a 1.5-2ul sample is recommended to
ensure that the liquid sample column is formed and the light path is
completely covered by sample.
Measurement Concentration Range
The NanoDrop 1000 Spectrophotometer will accurately measure
dsDNA samples up to 3700 ng/ul without dilution. To do this, the
instrument automatically detects the high concentration and utilizes
the 0.2mm pathlength to calculate the absorbance.
Detection
Limit
(ng/ul)
Approx.
Upper Limit
(ng/ul)
Typical Reproducibility
(minimum 5 replicates)
(SD= ng/ul; CV= %)
2
3700 ng/ul
(dsDNA)
3000 (RNA)
2400 (ssDNA)
sample range 2-100 ng/ul:
±
2
ng/ul
sample range >100 ng/ul:
±
2%
Unique Screen Features
5-1
