3.
On the Advanced
™
screen, press Adjust to open the Adjust screen.
4.
Optional: Press the Graph button to view the distribution of cells (live and/or
dead) based on size as you gate the count results (“Save as new protocol“ on
5.
Select the channel (Live or Dead) you wish to gate.
6.
Using the size, brightness, and circularity sliders, adjust the count parameters.
Note:
For a description of the count parameters and count parameter controls
(i.e., parameter sliders), see “Count parameters“ on page 15.
7.
When finished, press Done to save the changes to count parameters and return
to the Advanced
™
screen.
Press Cancel to return to the Results screen without saving the changes.
8.
On the Advanced
™
screen, press Count to recalculate your results with the new
count parameters.
9.
To save the changes to size, brightness, or circularity parameters to the current
profile or to create a profile with the new count parameters, see “Save as new
10.
To permanently save your results, see Chapter 7, “Save results“.
Chapter 4
Cell count and cell viability assays
Gate count results
4
28
Countess
™
II FL automated cell counter User Guide
