6.
Optional: To manually adjust the focus, press the Focus
™
button and use the
Focus
™
slider to bring your sample into focus as described in “Set nominal
7.
Press the Set button to set the focus and collapse the focus controls. Once the
focus has been set, the Set button on the focus slider becomes inactive,
confirming that the focus setting has been stored.
8.
To view your sample under a different light source, press the desired Light
source button. The instrument displays the sample in the selected channel
(brightfield or fluorescent).
In the example below, the sample is displayed in the GFP channel.
Note:
The light source buttons select the light channel (brightfield and/or
fluorescence) for sample illumination and are used when setting the exposure for
the selected channel (Steps 9 on page 32–11 on page 33); they do not determine
which channels are used for capturing the image.
9.
To set exposure, press Adjust to go to the Adjust screen.
Chapter 5
Fluorescence assays
Count cell fluorescence
5
32
Countess
™
II FL automated cell counter User Guide
