Fluorescence assays
Count cell fluorescence
Countess
™
II FL Automated Cell Counter equipped with the optional EVOS
™
light
cubes can be used for a variety of fluorescent applications, including simultaneous
counts of cells stained with two different fluorescent dyes, GFP and RFP expression,
and apoptosis and cell viability assays.
For instructions on installing EVOS
™
light cubes to your Countess
™
II FL Cell
Counter, see “Change light cube“ on page 54.
1.
Ensure that your fluorescent cell sample is homogeneously mixed.
2.
Load 10 µL of the fluorescent sample mixture per chamber into the sample slide
as described in “Load Countess
chamber slide“ on page 19. Let the sample
mixture settle for 30 seconds.
3.
Optional: Press the Profiles
button located on the upper left corner of the
screen to open the Profiles screen and load the desired profile as described in
4.
Insert the sample slide into the slide port (“Instrument exterior components“ on
page 11), making sure that the sample side is inserted completely into the
instrument. You will hear a soft click, if the slide is pushed in correctly.
5.
When the slide is inserted, the instrument automatically illuminates the sample,
sets the intensity of brightfield illumination, and auto focuses on the cells.
Note:
To turn off the Auto Lighting function, see “Define count parameters for
the fluorescent channels“ on page 17.
5
Overview
Count procedure
Countess
™
II FL automated cell counter User Guide
31
