Load profile
Profiles screen allows you to create and save up to 9 customized profiles. Each custom
profile defines the count parameters (size, brightness, circularity, and fluorescence
intensity) and automatic instrument functions (Auto Lighting and Auto FL
Threshold) for a consistent and streamlined workflow.
• You can access the Profiles screen from the Home, Capture, Results, Advanced
™
,
or Adjust screens.
• The current profile is displayed on the upper left corner of the Home, Capture,
Results, Advanced
™
, or Adjust screens.
• Automatic instrument functions (below) and count parameters (“Count
parameters“ on page 15) are defined in the Edit profile screen (see “Add/edit a
• The Default profile contains default count settings and cannot be edited.
• The count parameters specified in the selected profile are applied to all new cell
counts.
• If you have already performed a count, loading a new profile from the Results
screen applies the count preferences to the current counts results (total cells,
viability etc.) and to all new counts.
• If you change any setting that is saved as part of the protocol (size, brightness, or
circularity) on the Results screen, the profile name is appended with the (*)
symbol.
You can turn the Auto FL Threshold and Auto Lighting functions ON and OFF using
the Auto FL Threshold and Auto Lighting checkboxes in the Edit profile screen
(“Add/edit a profile“ on page 16).
• Auto FL Threshold: Automatically applies threshold in fluorescence channels to
subtract background fluorescence for improved analysis despite variable
background levels between samples. This function is available only for
instruments equipped with the optional EVOS
™
light cubes.
• Auto Lighting: Automatically illuminates the sample in the brightfield for
increased sample-to-sample consistency and decreased user-to-user variability.
Profiles screen
Automatic
instrument
functions
Chapter 3
Getting started
Load profile
3
14
Countess
™
II FL automated cell counter User Guide
