Count parameters are adjusted in the Edit profile screen using the parameter sliders.
Parameter sliders correspond to a single channel, which is selected using the channel
selection radio buttons located above the sliders.
• Size: As you move the slider up, the algorithm includes larger objects in the
count. As you move the slider down, only the smaller objects are counted.
• Brightness: As you move the slider up, the algorithm includes the brighter
objects in the count. As you move the slider down, only the dimmest of objects
are counted.
• Circularity: As you move the slider up, the algorithm includes more objects with
shapes other than circular in the count. As you move the slider down, only the
objects that are perfect circles are counted.
• Fluorescence intensity: As you move the slider up, the algorithm includes the
objects that fluoresce more brightly in the count. As you move the slider down,
only the dimmest of objects are counted.
• Size, brightness, and fluorescence intensity sliders are range sliders.
To adjust the upper and lower boundaries without changing the data range, drag
the slider by its middle section (i.e., the slider bar).
To adjust only the upper or the lower boundary, move the upper or the lower
handle in the desired direction. This will also change the range of values within
which the cells are counted.
• The circularity slider only sets a single threshold value; cells that fall below the
set value are counted, and cells that are beyond this range are excluded.
To adjust the threshold for circularity, drag the slider in the desired direction.
Count parameters
Chapter 3
Getting started
Load profile
3
Countess
™
II FL automated cell counter User Guide
15
