1.
On the Results screen, click More to open the Advanced
™
screen.
2.
Optional: To view your sample under a specific light source (brightfield and/or
fluorescent), select the desired Channels checkbox (brightfield in the example
above). You may display your sample in any or all of the available channels.
3.
To identify the cells that are counted in a specific channel, press the
corresponding boundaries button. Cells counted in the selected channel will be
circled on the screen with the same color as the selected channel.
In the example below, both the GFP and TxRed boundaries buttons are pressed
and the cells counted in the GFP and TxRed channels are marked with green and
red circles, respectively.
4.
To unmark the cells counted in a specific channel, press the corresponding
boundaries button again.
Identify cells
counted in
fluorescence
assays
Chapter 5
Fluorescence assays
Identify objects counted
5
Countess
™
II FL automated cell counter User Guide
35
