Thermo Scientific CryoStar NX70 series Operator'S Manual Download Page 99

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Thermo Scientific CryoStar NX70 Operator Guide

Problem

Possible Reason

Advice

1. Check knife holder and
specimen correctly clamped.

2. Ensure specimen is securely
attached to the
Cryocassette/chuck.

3. Temperature of specimen
incorrect.

Raise or lower temperature.

4. Blade not sharp.

Change blade.

Summary of Contents for CryoStar NX70 series

Page 1: ...Thermo Scientific CryoStar NX70 Product Code 956960 956970 956980 956990 957000 957010 957020 957030 957040 957050 957060 957070 Operator Guide 387928 Issue 10 ...

Page 2: ...Thermo Scientific CryoStar NX70Operator Guide 2 ...

Page 3: ...is up to date and relates to the condition of the product If necessary check with your local Thermo Fisher Scientific representative This document may not in whole or in part be copied photocopied reproduced translated or converted to any electronic or other form without prior written consent of Thermo Fisher Scientific All information contained in this manual is proprietary and confidential and t...

Page 4: ...ed greenhouse gases Refrigerant R404A R404A GWP value 3992 System charge weight 0 400 Kg System CO2 equivalent charge weight 1 597 tonnes EMC Statement This IVD equipment complies with the emissions and immunity requirements of IEC 61326 2 6 2012 and IEC 61326 1 2012 This equipment has been designed and tested to CISPR 11 2009 A1 2010 Class A It is intended for use in a laboratory environment by a...

Page 5: ...n your instrument This IFU is valid for the variants listed below Product Code Product Variant 956960 CryoStar NX70 HOMP 100V 60 Hz 956970 CryoStar NX70 HOMPD 100V 60 Hz 956980 CryoStar NX70 HOMPV 100V 60 Hz 956990 CryoStar NX70 HOMPDV 100V 60 Hz 957000 CryoStar NX70 HOMP 110 120V 60 Hz 957010 CryoStar NX70 HOMPD 110 120V 60 Hz 957020 CryoStar NX70 HOMPV 110 120V 60 Hz 957030 CryoStar NX70 HOMPDV ...

Page 6: ...ls are present Refer to the Material Safety Data Sheets for the products and always use Good Laboratory Practice Separate taking back of electrical and electronic instruments in the countries of the European Union This is to be applied in the countries of the European Union and other European countries with a separate collecting system within the waste management This product being an electro and ...

Page 7: ... Microtomeand Sectioning Specifications 15 Cold D Specification 16 Default Settings 16 CHAPTER3 INSTRUMENT SETUP 18 UNPACKING AND REPACKING 18 Safety Precautions 18 Unpacking Procedure 18 Repacking theInstrument 19 LOCATION SETUP 20 IDENTIFICATION OF PARTS 21 Overview 21 ConnectionsPanel 22 Cryochamber 23 Blade Holder 24 Specimen Head NX70 25 SET UP 26 Fitting or Exchanging theAnti Roll Plate 26 F...

Page 8: ...52 Time and Date 52 Cutting Mode Selection 53 Handwheel Brake 58 Multi Function Button 59 Menu 61 Chamber Light 62 Standby 63 Display Lock 64 Favourites 65 User Guide 67 SettingsMenu 69 Language 70 Time and Date 71 Start Time 74 Defrost 76 ImmediateDefrosting 76 Programming the Daily Defrost Cycle 78 Screen 80 Retraction 81 Data Log 83 Default Settings 84 Service 85 EMERGENCY STOP 86 Standard Emer...

Page 9: ...Sectioning Temperature Guidelinesfor Fresh Tissues 102 CHAPTER6 CLEANINGANDMAINTENANCE 103 CLEANING THE TOUCH SCREEN DISPLAY 103 SHUTTING OFF FOR CLEANING 103 CLEANING AND CARE OF THE BLADE HOLDER 103 EMPTYING THE DEFROSTING AND COLD D LIQUIDS 104 EXCHANGE THE VACUTOME FILTER BAG 105 ADJUSTMENT OF THE MOVABLE SPECIMEN HEAD JAW 107 TRANSPORTATION OF THE INSTRUMENT 108 Conditions for the Transportat...

Page 10: ...loyed and consideration must be given to the potential for hazard when dealing with these chemicals Do not use the instrument in close proximity to strong electromagnetic radiation as these may interfere with the proper operation The electromagnetic environment should be evaluated prior to operation of the device Good Laboratory Practice must be used when handling tissue samples to prevent cross c...

Page 11: ...nt where chemicals are stored or likely to leak into in a single fault condition The operator is fully aware of the contents of the specification documents detailing the properties of the chemicals they are using The operator has carried out any legally required assessment of chemicals used and is using Good Laboratory Practice Environment This instrument complies with the European Union s Waste E...

Page 12: ...p maintain your instrument in an optimal operating condition Warranty provisions necessarily vary to comply with differences in national and regional legislation Specific details can be found in the delivery documentation or from your dealer or representative Please note that your warranty may be invalidated if This instrument is modified in any way or not used as intended by Thermo Shandon Limite...

Page 13: ... the instructions given in this manual Any other use of this instrument is considered as improper operation Instrument Features The CryoStar NX70 is a precision engineered ergonomically designed high performance cryostat in modular design with a stainless steel cooling chamber Some of its features are Knife Blade Holder cooling Specimen cooling Ergonomic height adjustment with integrated ergonomic...

Page 14: ...Use Only Temperature Operating Limits 5 C to 35 C 41 F to 95 F Temperature Recommended Operation 15 C to 30 C 59 F to 86 F Note Performance may deteriorate when operated outside of this range Temperature Transport and Storage 20 C to 40 C 4 F to 122 F 70 C 158 F for short exposure Relative Humidity Max 80 RH up to 31 C Decreasing linearly to 50 RH at 40 C Altitude Up to 2000 m 6 500 ft Pollution D...

Page 15: ...s Range 0 5 100 µm Fine Section Thickness Resolution 0 5 2 µm in 0 5 µm steps 2 µm 10 µm in 1 µm steps 10 µm 20 µm in 2 µm steps 20 µm 50 µm in 5 µm steps 50 µm 100 µm in 10 µm steps Trimming Thickness Range 5 500 µm Trimming Thickness Resolution 5 10 µm in 5 µm steps 10 µm 100 µm in 10 µm steps 100 µm 200 µm in 20 µm steps 200 µm 500 µm in 50 µm steps Specimen Retraction Return Travel 20 µm Verti...

Page 16: ...acutome Filter approximately 500 ml Cold D Specification Reservoir Volume 1000 ml Recommended Medium Sanosil S010 5 H2O2 Default Settings Counter Mode Speed V 0 Multi Counter 2 Operating Mode Continuous Retraction ON Language English Fine 0 5 µm Trim 5 µm Joystick Calibration Calibrated Chamber Light 100 Screen Brightness 100 Brake ON Stepper Motor Initialization Yes Blade Temperature 15 C Specime...

Page 17: ...Automatic ON 00 00 Night Standby Mode 1h After 1 hour the Cryostat enters the Standby Mode automatically Kept temperature 15 C Sleep Mode 3h After 3 hours the Cryostat enters the Sleep Mode automatically Kept temperature 10 C Power savings Approximately 30 Start Time 06 00 a m Section Counter 0 Section Sum 0 ...

Page 18: ...tions are retained for future use The CryoStar NX70 is delivered on a pallet The sides of the instrument are encased in cardboard and cushioned with foam spacers inside the container A wooden cover protects the top of the instrument In unpacking be aware of the weight 200 Kg 440 lbs of the instrument Safe handling of the installation requires the involvement of two people Handling Place the crate ...

Page 19: ...andard accessories with the bails To move the instrument from the pallet stand BEHIND NOT in front of the instrument Carefully roll the instrument off the pallet and on to the floor Move the instrument to the desired location while leaving space on the left and right hand Keep all packing and transportation materials together in case of future need In the future if the instrument is transported by...

Page 20: ...er socket must be accessible at all times The location must be free from draughts open doors or air conditioning systems The location must not expose the cryochamber to direct sunlight Enough room for height adjustment Note These measures are intended to reduce the build up of frost and maintain consistent cooling temperatures resulting in more favourable working conditions Locations which have hi...

Page 21: ...ator Guide 21 Identification of Parts Overview 1 Cryochamber 6 Connections Panel 2 Cryochamber Window 7 Handwheel 3 Touch Screen 8 Handwheel Mechanical Brake 4 Cold D Reservoir Cover 9 Water Drain Reservoir Cover 5 Emergency Stop Button ...

Page 22: ...yoStar NX70Operator Guide 22 Connections Panel 10 Power Switch 13 X1 RJ45 port for Service purposes only 11 Power Socket 14 X2 USB port for service purposes only 12 Rating and Serial Number Label 15 X3 Foot Pedal Connector ...

Page 23: ...Cutting Speed Control 17 Trim Button 24 Motorized Cutting Start Stop Button 18 Joystick 25 Handwheel Electronic Brake Button 19 Specimen Head 26 Vacutome Filter Cover 20 Blade Holder with Anti Roll Plate 27 Vacutome Filter Cover Release Knob 21 Vacutome 28 Section Waste Tray 22 Mode Selection Button ...

Page 24: ...tar NX70Operator Guide 24 Blade Holder 29 Lateral Adjustment Lever 33 Blade Holder Base 30 Anti Roll Plate 34 Anti Roll Plate Adjustment 31 Blade Clamping Lever 35 Clearance Angle Adjusting Screw 32 Top Stage Angle Adjustment ...

Page 25: ...Operator Guide 25 Specimen Head NX70 36 Y axis Fine Adjustment Knob 39 Specimen Head Clamping Lever 37 Specimen Chuck Release Lever 40 Upper Specimen Chuck Jaw Static 38 X Axis Fine Adjustment Knob 41 Lower Specimen Chuck Jaw Moving ...

Page 26: ...pecimen chucks which should be kept at room temperature The heated sliding window should be closed during periods of inactivity and while preparing specimens to avoid warm air getting into the chamber This will result in more favourable working conditions and a lower frost build up Ensure that the Vacutome filter is properly inserted in the filter unit and is suitably maintained see Cleaning and M...

Page 27: ...e and specimen or changing the specimen The blade holder should be fitted with low profile blades 80mm x 8mm 35 facet angle as standard To fit or exchange low profile blades Use only the front handle to move the anti roll plate to the side Pull the blade clamping lever towards the front of the instrument to release the clamping plate Carefully slide a low profile blade into the middle of the slot ...

Page 28: ...r in a safe way Remove the used blade with the magnetic blade tool Use only the front handle to move the anti roll plate to the side Pull the blade clamping lever towards the front of the instrument to release the clamping plate Move the magnetic blade tool over the blade Carefully lift the blade out of the blade holder and dispose of it in accordance with laboratory regulations ...

Page 29: ...no longer usable the upper part of the blade holder can be moved without removing the blade To move the upper part of the blade holder Loosen the lateral adjustment lever The upper part of the blade holder should now be free to move left and right Move it either to the left or to the right until the desired area of the blade is directly below the specimen head Move the lateral adjustment lever for...

Page 30: ...ting angle is 10 Loosen the clearance angle adjustment screw on the lower left site of the blade holder Gently push both sides of the blade holder base to select the desired angle The surface of the blade holder base might be cold Use gloves if necessary Note The clearance angle can be read on the scale on the left side on the upper part of the blade holder Note 10 is indicated by the longer incre...

Page 31: ...Thermo Scientific CryoStar NX70Operator Guide 31 Re tighten the clearance angle adjusting screw to fix the selected clearance angle ...

Page 32: ...e adjustment of the anti roll plate is carried out using the knurled screw To raise the height of the Anti Roll Plate turn the screw anti clockwise To lower the height of the Anti Roll Plate turn the screw clockwise Note Always adjust the height of the Anti Roll Plate in small increments ...

Page 33: ...amp and orient the specimen Loosen the specimen chuck release lever and insert the specimen chuck into the jaws of the specimen head Release the specimen chuck release lever again to allow the jaws to grip the chuck Loosen the specimen head clamping lever to allow adjustment of the specimen parallel to the cutting edge Use the fine adjusting knobs to orient the specimen on the x and y axes ...

Page 34: ... chuck can also be rotated by 360 as described below Loosen the specimen head clamping lever slightly Rotate the chuck as required Move the specimen head clamping lever back to its original position After the specimen is adjusted set the cutting window limits see Setting the Cutting Window Use the motorized coarse feed to position the blade close to the specimen Tilt the joystick forwards to move ...

Page 35: ... To avoid frosting of the chamber Cold D should not be used more than 2 times per day plus one cycle overnight Filling the Cryostat with DisinfectantSolution Sanosil To load the cryostat with disinfectant Open the Cold D reservoir cover to access the filling inlet of the disinfectant tank Before filling the tank check the shelf life and the hazardous substance warnings on the disinfectant Note Alw...

Page 36: ...Thermo Scientific CryoStar NX70Operator Guide 36 Empty the contents of one litre Sanosil into the tank Close the reservoir cover ...

Page 37: ...ostat as the compressor needs high surge currents when started Do not use multi socket power outlets with small wire sizes for the supply of the instrument Insert the supplied power cord into the power socket and then connect the power cord to the mains supply Install the blade holder if necessary Close the heated window Turn on the power switch Note The default setting of the blade holder and spe...

Page 38: ...ons on the touchscreen Main Screen The Main screen appears after the instrument has been switched on 1 Disinfection Cold D Function 9 Favourite Temperatures 2 Cryobar 10 Vacutome Settings 3 Menu 11 Height Adjustment 4 Trimming Thickness 12 Handwheel Brake On Off 5 Blade Temperature 13 Multi Function Button 6 Specimen Head Temperature 14 Cutting Mode 7 Fine Section Thickness 15 Time or Date Service...

Page 39: ...he previous menu without saving any changes Arrow keys The Arrow keys are used in several menus to increase or decrease values Use the arrow keys to adjust the value to the required setting 1 Decreases the selected value by multiple steps 3 Increases the selected value by 1 step 2 Decreases the selected value by 1 step 4 Increases the selected value by multiple steps Note The number of steps by wh...

Page 40: ...ure of 60 C The Cryobar remains active for approximately 10 minutes The specimen is frozen onto the specimen chuck using a freezing compound and the Cryobar To ensure optimal adhesion of the specimen to the chuck store the chucks at room temperature prior to use Press the Cryobar button on the Main screen to activate it The Cryobar button is highlighted in blue when the function is activated To tu...

Page 41: ...ction Thickness Trim Section Thickness Note The selected function is displayed highlighted on the Main screen Turn the joystick knob left to decrease the selected thickness Turn the joystick knob right to increase the selected thickness For details of the thickness steps refer to the Technical Specifications section Using the touch screen display To set the trimming section thickness Press the Tri...

Page 42: ... to exit back to the Main screen without saving the changes To set the fine section thickness Press the Fine Section Thickness button on the Main screen The Fine Section screen will be displayed Either select one of the pre set values 100µm 50µm 10µm or 5µm or adjust the value using the arrow keys For details of the thickness steps refer to the Technical Specifications section Press OK to confirm ...

Page 43: ...mperature button on the Main screen The Temperature Specimen Head screen will be displayed Either select one of the pre set temperatures 40 C 30 C 20 C or 10 C or adjust the temperature using the arrow keys Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Note The new set temperature is displayed in wh...

Page 44: ...played Either select one of the pre set temperatures 35 C 30 C 20 C or 10 C or adjust the temperature using the arrow keys Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Note The new set temperature is displayed in white on the Main screen until the temperature is reached ...

Page 45: ... The lower limit should now be set and the Start button is highlighted in yellow To set the upper limit of the cutting window Continue turning the handwheel clockwise so that the upper edge of the specimen is positioned just below the blade edge Press the End button The End button is highlighted in yellow Next Steps Press OK to confirm the setting and to return to the Main screen Press the Back bu...

Page 46: ...he pre set Favourites Press the Favourites button on the Main screen The Favourites sliding menu will appear Press the desired favourite to select it The sliding window disappears and the selected Favourite is highlighted in yellow To program favourites refer to the Setting Preferences Favourites section Note The sliding menu disappears after approximately 3 seconds if no Favourite is selected ...

Page 47: ...g window Outside of the cutting window the selected vacuum is turned off by means of a valve After 10 minutes of inactivity the vacuum will automatically switch off When activity resumes the vacuum will automatically switch on The vacuum takes approximately 2 seconds to reach full efficiency The Vacutome system is provided with two different length nozzles The longer nozzle is optimized for use in...

Page 48: ...and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Note The Vacutome suction setting can also be adjusted by turning the Joystick as long as the Vacutome screen is displayed Turn the Joystick to the left to decrease the suction value in 1 increments Turn the Joystick to the right to increase the suction value in 1 increments Note When ...

Page 49: ...leaning mode Press the Vacutome button on the Main screen The Vacutome screen will be displayed Press Cleaning Mode to activate this mode Note The Cleaning Mode button turns yellow to indicate that this function is now activated The cleaning mode will switch of automatically after 10 minutes Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the ...

Page 50: ... cooling chamber Turning Off the cleaning mode To deactivate the cleaning mode Press the yellow Vacutome button on the Main screen Press the yellow Cleaning Mode button on the Vacutome settings screen the button will now appear white Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Re attach the Vacuto...

Page 51: ...he instrument when the height adjustment is carried out to avoid damage to the instrument Ensure that the power cord is free to move To adjust the height of the cryostat Press the Height button on the touch screen An adjustment menu will appear Use the arrow buttons to raise or lower the cryostat to the required level Press OK to confirm the adjustment The sliding menu disappears after approximate...

Page 52: ...nction Bar is located at bottom of the Main screen 1 Time Date Service Message 3 Multifunction Button 2 Cutting Mode 4 Brake Time and Date To toggle between Time or Date indication Press the Time Date button Note When there is an outstanding service message a wrench symbol is show instead of the Date Time button Press the Wrench symbol to see the service message ...

Page 53: ...stopped in every operating mode by pressing the emergency stop button or the emergency stop in the optional foot pedal To continue sectioning pull out the emergency stop button or unlock the emergency stop in the foot pedal The cutting mode can be changed in three ways Via the Main screen in the cutting mode submenu or via the mode button on the right touch panel To switch between the cutting mode...

Page 54: ...the Main screen The Menu screen will be displayed Press the Cutting Mode button The Menu Cutting Mode screen will be displayed Press on the desired cutting mode Continuous Interval Single or Multi Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes ...

Page 55: ...carried out with the specimen head stopping in the down position Press either the Start Stop button twice or press the foot pedal twice to carry out a single cutting stroke Mode Interval The cutting movement is carried out as long as the Start Stop button or the foot pedal is pressed The movement can be stopped in any position Press either the Start Stop button twice or press the foot pedal twice ...

Page 56: ...st selected the default number of strokes is two To set the number of strokes for the Multi Stroke Mode Press the Menu button on the Main screen The Menu screen will be displayed Press the Cutting Mode button The Menu Cutting Mode screen will be displayed Press the Multi button to highlight it Use the arrow keys to select the number of strokes ...

Page 57: ...trokes as described above Press either the Start Stop button twice or press the foot pedal twice to start sectioning The cutting movement stops automatically after the pre set number of strokes have been carried out Mode Continuous Stroke An unlimited number of strokes will be performed when this mode is selected To start the continuous stroke mode Press either the Start Stop button twice or press...

Page 58: ...activate the handwheel mechanical brake To activate the Handwheel Brake Press the Brake button on the Main screen or press the Brake button on the right touch pad Note The Brake button is highlighted if the handwheel brake is activated To deactivate the Handwheel Brake Press the red highlighted Brake button on the Main screen or the Brake button on the right touch pad ...

Page 59: ... the blade holder Section Thickness Sum This button indicates the total section thickness sum of the actual mounted specimen To reset this value press this button and keep it pressed for approximately 3 seconds Note It is recommended to reset the sectionthickness sum if a new specimen is mounted in the specimen head for sectioning Motorized Cutting Drive The motorized cutting drive facilitates rou...

Page 60: ...ide of the cutting window a higher return speed determined by the system is used Use either the operating controls or the foot pedal if fitted to turn on off the drive The cutting speed can be varied to allow it to better suit to the properties of the specimen Setting a cutting speed too high in combination with a high section thickness may result in a warning screen Section Counter The section co...

Page 61: ... provides access to submenus for adjustments settings and the user guide To enter the Menu screen Press the Menu button The Menu screen will be displayed Press the Back button to return to the Main screen Press the desired function button to access the respective submenu ...

Page 62: ...ber Light button The Menu Chamber Light screen will appear Select one of the pre set values 25 50 75 or 100 or adjust the brightness using the arrow keys Press OK to confirm the setting and to return to the Main screen Press the Back button twice to exit back to the Main screen without saving the changes Note The chamber illumination can be adjusted from the lowest 1 to the highest 100 level ...

Page 63: ...lade holder and specimen head during Standby When the cryostat enters the sleep mode the temperature for the blade holder is set to 10 C and for the specimen head to 10 C In Standby mode the chamber light switches off and the touchscreen display will dim To enter the Standby mode Press the Menu button on the Main screen The Menu screen will appear Press the Standby button to switch the instrument ...

Page 64: ...e settings of the cryostat To activate the Display Lock Press the Menu button on the Main screen The Menu screen will appear Press Display Lock to activate the display lock immediately To deactivate the Display Lock Press the Lock Symbol three times Note The symbol changes its colour red yellow green each time it is pressed ...

Page 65: ...ting is altered the cryostat will require a period of time to reach the set temperature Observe the readings on the display and begin sectioning when the pre set value is reached To program Favourites Press the Menu button on the Main screen The Menu screen will appear Press Favourites to enter the menu The Menu Favourites screen will appear 1 Pre set 3 Title Box 4 Specimen Head Temperature 2 Pre ...

Page 66: ...board screen will appear Use the upper left button to toggle between capital letters small letters and numbers Erase letters or numbers by pressing the Delete button Type in the new name for the favourite for example Muscle Fat Bone Press OK to confirm the setting and to return to the Favourites menu Press the Back button to exit back to the Favourites menu without saving the changes Note If the v...

Page 67: ...the Menu button on the Main screen The menu screen will appear Press the User Guide button The Menu User Guide screen will appear Use the scroll bar on the right side or the two arrow keys to scroll through the manual Press the Home button to return to the first page of the User Guide Use the arrow keys to browse forward or backwards through the User Guide ...

Page 68: ...Thermo Scientific CryoStar NX70Operator Guide 68 Press the Back button to return to the settings menu screen Press the Back button twice to return to the Main screen ...

Page 69: ...o enter the Settings menu Press the Menu button on the Main screen The menu screen will appear Press the Settings button The Menu Settings screen will appear Press the Back button to return to the Menu screen Press the Back button twice to return to the Main screen ...

Page 70: ...Press the Settings button The Menu Settings screen will appear Press the Language button The Menu Settings Language screen will appear Select the desired language by pressing the respective button Press OK to save the changes and to return to the Main screen Press the Back button three times to return to the Main screen without saving the changes ...

Page 71: ...he Main screen The Menu screen will be displayed Press the Settings button The Settings Menu screen will be displayed Set the Date Press the Date button The Menu Settings Date screen will appear Set the Day Press the Day button to highlight it Use the arrow keys to select the desired day and date Set the Month Press the Month button to highlight it Use the arrow keys to select the desired month ...

Page 72: ...n screen without saving the changes Change to ISO format Press the ISO button to toggle between the full date format and the ISO format Press OK to confirm the setting and to return to the Main screen Press the Back button three times to exit back to the Main screen without saving the changes Setting the Time Press the Menu button on the Main screen The Menu screen will be displayed Press the Sett...

Page 73: ...he hour Press the Hour button to highlight it Use the arrow keys to select the desired hour Set the time format Press the 12 24 button to toggle between the 12 and 24 hour modes Note The 12 24 button is highlighted in yellow if the 12h mode is active Press OK to confirm the setting and to return to the Main screen Press the Back button three times to exit back to the Main screen without saving the...

Page 74: ...Time Press the Menu button on the Main screen Press the Settings button The Menu Settings screen will appear Press the Start Time button The Menu Settings Start Time screen will appear In this menu the user can specify the day s and the exact time when the cryostat should start automatically Note The pre set start time is from Monday to Sunday at 06 00 a m To set the Day Press the Day button ...

Page 75: ...kdays on which the cryostat should not start automatically Note Deactivated weekdays will turn white Press the Hour and Minute button and use the arrow keys to adjust the time at which the cryostat should start When all settings have been done press OK to save the settings and to return to the Main screen or press the Back button three times to return to the Main screen ...

Page 76: ...trument the specimen might be damage Note Set the time of the daily defrosting on the control panel The period of the defrosting takes approximately 1 hour and varies according to the thickness of the frost Possible frost build up on the microtome or on the knife carrier is not removed by defrosting the instrument However the frost recedes after defrosting as now the evaporator is fully efficient ...

Page 77: ... defrosting immediately The Defrosting screen will appear showing the progress of the defrosting and the actual temperature of the evaporator To abort the defrosting Press the Abort button on the Defrosting progress screen The Main screen will appear displaying an error message Press the OK button to remove the error message Note The Defrosting cycle can be aborted at any time ...

Page 78: ...working hours to avoid delays in the workflow To program the Daily Defrost Cycle Press the Menu button on the Main screen The Menu screen will be displayed Press the Settings button The Menu Settings screen will appear Press the Defrost button The Menu Settings Defrost screen will be displayed Press the Time button twice The Menu Settings Defrost Time screen will appear ...

Page 79: ...ing for the daily defrost cycle is daily at 00 00 a m To change the standard setting press the Hour and or the Minute button Adjust the time by using the arrow keys Press the OK button to confirm your settings Press the Back button three times to return to the Main screen ...

Page 80: ...ngs button The Menu Settings screen will appear Press the Screen button The Menu Settings Screen will be displayed Select one of the pre set values 25 50 75 or 100 or adjust the brightness using the arrow keys Press OK to confirm the setting Press the Back button three times to return to the Main screen without saving the changes Note The chamber illumination can be adjusted from the lowest 1 to t...

Page 81: ...on on the Main screen The Menu screen will be displayed Press the Settings button The Menu Settings screen will appear Press the Retraction button The Menu Settings Retraction screen will be displayed Press the OK button to turn off the Retraction and to return to the Main screen Press the Back button three times to return to the Main screen without saving the changes ...

Page 82: ...traction Enter the Retraction menu screen as described above Press the OK button on the Menu Settings Retraction screen to turn on the Retraction and to return to the Main screen Press the Back button three times to return to the Main screen without saving the changes ...

Page 83: ...displayed Press the Settings button The Menu Settings screen will appear Press the Data Log button The Menu Settings Data Log screen will be displayed Use the scroll bar on the right side to scroll through the Data Log Insert the USB Stick Note The Export button is now highlighted in white Press the Export button to transfer the log data to the USB Stick Press the Back button three times to return...

Page 84: ...Menu Settings screen will appear Press the Default Settings button The Menu Settings Default Settings screen will be displayed Press the OK button to restore the default settings and to return the Main screen Press the Back button three times to return to the Main screen without restoring the default settings Note A message appears on the Main screen to inform the user that the default settings ha...

Page 85: ...witch the text off Enter the Menu Settings Default Settings screen as describedabove Uncheck the box and press the Back button three times to save the changes and to return to the Main screen Pressing OK will reset the instrument to the default settings Service This submenu is only for service purposes A password is required to enter the Service menu ...

Page 86: ...n to immediately stop the motorized cutting drive Note When the emergency stop is activated the handwheel brake button in the display and the handwheel brake button on the right keypad light up and a red button are shown onthe display To reset the emergency stop function pull out the red emergency stop button The motorized cutting drive can then be started again ...

Page 87: ...ll remain active as long as the foot pedal is engaged The motorized cutting drive can then be started again Cooling Processes Specimen and blade holder cooling Integrated refrigeration systems allow the blade holder to be cooled to 35 C and the specimen head to be cooled to 50 C Specimen stations are located on the left side inside the Cryochamber These specimen stations passively cool specimens t...

Page 88: ...ion Front Rear Lateral view into instrument Note The retraction prevents the surface of the specimen being damaged during the return stroke of the specimen head The retraction can be switched off see Switching the Retraction On Off The retraction distance required is 20 microns and cannot be changed ...

Page 89: ... the trimming thickness setting is selected by using the joystick Note Trimming thickness is shown highlighted on the display Turn the hand wheel in a clockwise direction to begin trimming Press the Trim button to move forward the blade holder by the pre set trim value The hand wheel does not need to be turned for this function Note The blade holder will move forwards by the pre set trim thickness...

Page 90: ...blade Turn the hand wheel in a clockwise direction or use the motorized cutting drive to carry out sectioning Note The section slides into the space between the blade and the anti roll plate Use the handle to move the anti roll plate assembly away from the specimen The section should remain on the clamping plate Arrange specimen section gently with a brush Transfer the specimen onto a glass micros...

Page 91: ...hand wheel brake has to be activated For your personal safety both electronic and mechanical hand wheel brakes must always be activated when working on the specimen head or blade carrier Electronic Handwheel Brake To activate the electronic Handwheel Brake Press the Brake button on the Main screen The Brake button is highlighted in red to indicate that the electronic brake is active Note The motor...

Page 92: ...mechanical Handwheel Brake Bring the handwheel handle into a 12 o clock position The switch for the handwheel brake can now be pressed into the locked position Note If the switch is not in the correct positionto lock keep the switch pressed and move the handwheel slowly forward and backwards until the switch is locked Starting the cutting motor drive is not possible when the instrument is turned o...

Page 93: ... Cold D cycle Note If the window is not closed the warning Window not closed will be displayed Before starting the Cold D function remove all specimens from the Cryochamber Press the Disinfect button on the Main screen The Disinfection screen will appear Press OK to start the disinfection immediately Press the Back button to return to the Main screen without starting the disinfection Note Pressing...

Page 94: ...a Cold D cycle These by products of the Cold D cycle will normally evaporate from the Cryochamber within a period of three hours The liquid droplets can be removed with a paper towel and if necessary any icing can be removed with an alcohol spray Neither the liquid droplets nor the icing will prevent the use of the cryostat immediately after completion of a Cold D cycle Any occasional build up of ...

Page 95: ...ion To abort the disinfection process Press the Abort button on the Disinfection Remaining Time screen Note If the Disinfection cycle was aborted an error message appears on the Main screen Press the OK button to remove the error message An interrupted Cold D cycle will not adequately disinfect the Cryochamber However the instrument can be used immediately afterwards ...

Page 96: ...ture Press the Menu button on the Main screen Press the Settings button The Settings Menu screen will be displayed Press the Disinfection button The Menu Settings Disinfection screen will appear In this menu the user can specify the day s and the specific time when the Cold D process should automatically be carried out To set the Day Press the Day button The Menu Settings Disinfection day selectio...

Page 97: ...nfection should not be carried out Note Deactivated weekdays will turn white Press the Hour and Minute button and use the arrow keys to adjust the time at which the Cold D cycle should start When all settings have been done press OK to save the settings and to return to the Main screen Press the Back button to return to the Setting screen without saving the changes ...

Page 98: ...rls after lifting ARP 1 Anti Roll plate clamp plate too warm Lower chamber temperature Tip use freezer spray on clamp plate and blade to confirm 2 Static electricity in chamber 3 Blade blunt Change blade Sections tear or crack 1 Specimen too cold Raise temperature 2 Blade damaged or dirty Change blade 3 Specimen frozen too rapidly or specimen overly large Specimen and section chatter 1 Knife holde...

Page 99: ...e 99 Problem Possible Reason Advice 1 Check knife holder and specimen correctly clamped 2 Ensure specimen is securely attached to the Cryocassette chuck 3 Temperature of specimen incorrect Raise or lower temperature 4 Blade not sharp Change blade ...

Page 100: ...e Optimum Temperature Ranges for Sectioning Different Types of Fresh Specimens Allow time for the temperatures within the chamber to stabilise Select the appropriate freezing compound Neg50 Avoid excessive use of freezing compound Note If the specimen was frozen with liquid nitrogen or similar freezing techniques the specimen must be allowed to adjust to the cutting temperature Adjustment of prope...

Page 101: ...ccording to the size of the specimen and the selected section thickness Ensure that the specimens are cooled sufficiently to avoid that sectioning waste sticking and blocking the suction hose of the Vacutome Note If the sectioning waste blocks the suction hoses of the Vacutome it can be removed by using the attached hose cleaner During this cleaning the Vacutome should be operated with maximum pow...

Page 102: ...t with Fat 35 C 30 C or below Cervix 25 C to 30 C 20 C Connective Tissue 25 C 16 C Gut 25 C 20 C Heart 25 C 18 C Kidney 25 C 15 C Lip 25 C 13 C Liver 25 C 13 C Lung 25 C 15 C Lymph Node 25 C 13 C Muscle 25 C 16 C Omentum 35 C 35 C Ovary 25 C to 30 C 20 C Pancreas 25 C to 30 C 20 C Prostate 30 C 20 C Skin 25 C 16 C Skin with fat 30 C to 35 C 25 C Spleen 25 C 16 C Testis 25 C 10 C Thyroid 25 C 15 C ...

Page 103: ...e the vacuum hose from the front side of the blade carrier Attach the cleaning nozzle to the Vacutome hose Remove the filter unit and dispose of the Vacutome filter together with the section waste and or the micro filter according to the respective lab regulations The warming up of the microtome chamber can actively be accelerated by softly using a hair dryer Clean wash and dry the cooling chamber...

Page 104: ... Disinfection Procedure Note The capacity of the bottle is 2 5 Litres approximately 0 66 Gallons It is recommended to empty and disinfect the bottle once a week during the routine cleaning procedure To empty the Defrosting Liquid Bottle Open the front door and pull out the bottle from the rack Dispose of the liquid in accordance to the laboratory regulations and clean it Note After cleaning the bo...

Page 105: ...ion waste can be hazardous Dispose of the used Vacutome filter bag in accordance to the laboratory regulations The Vacutome Filter Bag is placed under the cover on the right hand site of the Cryochamber Open the lever of the cover Remove the Vacutome hose 1 and lift off the cover 2 Clean the Vacutome hose with a flexible brush Slip off the old Vacutome filter and replace it by a new one ...

Page 106: ...o Scientific CryoStar NX70Operator Guide 106 Clean and disinfect the empty Vacutome filter chamber with disinfectant Replace the cover rear end first Reattach the Vacutome hose Lock the cover with the lever ...

Page 107: ... procedure until the right clamping tension is reached After the final shutdown of the instrument we recommend to contact a local recycling company for the disposal according to the national applicable regulations To be applied in the countries of the European Union and other European countries with a separate collecting systemwithin the waste management The marking of the product and or the respe...

Page 108: ...om the Cryochamber Dispose of the defrosting and Cold D liquids in accordance to the laboratory regulations and clean the bottle see Emptying the Defrosting and Cold D Liquids Remove the defrosting liquid bottle Note Clean and disinfect all accessories according to the respective applicable lab regulations and transport them in dry condition Any transportation of the instrument requires original p...

Page 109: ...der Remove the section waste tray the brush shelf the blade holder and all other accessories as well as tools from the cryochamber Dispose of the defrosting and Cold D liquids in accordance to the laboratory regulations and clean the bottle see Emptying the Defrosting and Cold D Liquids Remove the defrosting liquid bottle Note Clean and disinfect these accessories according to the respective appli...

Page 110: ... Extractor 524510 Specimen Chucks Ø 20 mm 715530 Ø 30 mm 715600 Ø 30 mm red 715870 Ø 30 mm green 715880 Ø 30 mm blue 715890 Ø 30 mm gold 715900 Ø 40 mm 715610 Ø 60 mm 715620 Cryo Moulds 10 mm 570400 15 mm 570380 22 mm 570390 Brushes Brush 334000 Small brush bevelled 334180 Large brush 334170 Neg 50 Frozen Section Medium 2 x 118 ml bottles 6502 6 x 118 ml bottles 6506 Cryomatrix embedding resin 4 x...

Page 111: ...n Oil 1 x 100 ml bottle 350110 1 x 250 ml bottle 350120 Anti Roll Plate 449980 Knife Guard A78930078 Allen Key 5 mm 362260 Allen Key 6 mm 362120 Waste Tray 512990 Brush Shelf 410540 Hose Extension w nozzle Vacutome 435560 Adjusting pin 242530 UK power cord 313940 European power cord 313960 US power cord 313950 Operator guide English 387928 Operator guide German 388125 Operator guide Italian 388126...

Page 112: ...s bacterial spores Disinfection Procedure for the CryoStar cryostat Wear appropriate protective clothing double gloves gown mask and protective eye wear Remove all disposable blades and knives from the chamber and disinfect them separately Using a paper towel remove all tissue trimmings and waste material from the cryostat chamber Bring the cryostat chamber to roomtemperature before beginning the ...

Page 113: ...Thermo Scientific CryoStar NX70Operator Guide 113 Appendix C Cold Disinfection Certificate ...

Page 114: ...ion 53 Cutting Movement 88 Cutting Window 45 D Data Log 83 Default Settings 16 84 Defrost 76 Disinfection Cold D Function 93 Display Lock 64 E Electrical Specifications 14 Electronic Handwheel Brake 91 EMC Statement 4 Emergency Stop 86 Emptying the Defrosting and Cold D Liquids 104 Environment 11 Environmental Specifications 14 Exchange the Vacutome Filter Bag 105 Exchanging Low Profile Blades 27 ...

Page 115: ...8 Sectioning Temperature Guidelines for Fresh Tissues102 Select a Favourite 46 Service 85 Set the Blade Temperature 44 Set Up 26 Setting Preferences 40 Settings Menu 69 Shutting Off for Cleaning 103 Specimenand blade holder cooling 87 Specimen Head NX70 25 Specimen Orientation 33 Specimen Setup 33 Standard Emergency Stop Function 86 Standby 63 Start Time 74 Successful Sectioning 100 Switching On 3...

Page 116: ...Thermo Scientific CryoStar NX70Operator Guide 116 ...

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