Thermo Scientific AFC-3000, Operating Instructions Manual

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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Operating Instructions Page i
Table of Contents
1 Introduction ................................................................................................................... 1
1.1 How to Use This Manual ........................................................................................... 1
1.2 Safety Information ..................................................................................................... 2
1.2.1 Symbols on the Fraction Collector and in the Manual ...................................... 2
1.2.2 Safety Precautions .............................................................................................. 3
1.2.3 Consignes de Sécurité ........................................................................................ 6
1.3 Intended Use ............................................................................................................ 10
1.4 Federal Communications Commission (FCC) Note ................................................ 11
2 Overview ...................................................................................................................... 13
2.1 Unit Description ....................................................................................................... 13
2.2 Operating Principle .................................................................................................. 14
2.3 Fraction Collector Configurations ........................................................................... 15
2.4 Front Panel Elements ............................................................................................... 17
2.5 Rear Panel ................................................................................................................ 18
2.5.1 Power Switch ................................................................................................... 19
2.5.2 USB Port .......................................................................................................... 19
2.5.3 RS-232 (Serial) Port ......................................................................................... 19
2.5.4 Auxiliary I/O Connection ................................................................................ 19
2.6 Universal Fraction Tray, Racks and Tubes .............................................................. 20
2.7 Chromeleon Software .............................................................................................. 21
3 Installation ................................................................................................................... 23
3.1 Facility Requirements .............................................................................................. 23
3.2 Unpacking ................................................................................................................ 24
3.3 Installing the Fraction Collector .............................................................................. 26
3.3.1 Mounting the Diverter Valve Assembly .......................................................... 27
3.3.2 Diverter Valve Connections ............................................................................. 28
3.3.3 Attaching the Rinse Station ............................................................................. 29
3.3.4 Connecting the Drain Tubing .......................................................................... 29
3.3.5 Placing Fraction Racks on the Universal Drip Tray ........................................ 30
3.3.6 Adjusting the Drop Former Height .................................................................. 33
3.4 Connecting the Fraction Collector ........................................................................... 35
3.4.1 Connecting the External Desktop Power Supply ............................................. 35
3.4.2 General Communications Information ............................................................ 36
3.4.3 Communication via RS-232 ............................................................................. 36
3.4.4 Communication via USB ................................................................................. 36
3.5 Setting Up the Fraction Collector in Chromeleon ................................................... 39
3.5.1 Installing the Fraction Collector ...................................................................... 39
3.5.2 Configuring the Fraction Collector .................................................................. 40
4 Verifying Installation .................................................................................................. 43
4.1 Overview of Actions ................................................................................................ 43
4.2 Inspecting Components Prior to Fluidic Operation ................................................. 43