Thermo Scientific AFC-3000, Operating Instructions Manual

Page: 16 / 88

UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Page 10 Operating Instructions
1.3 Intended Use
For Research Use Only. Not for use in diagnostic procedures.
The device is designed to be operated only be qualified and authorized personnel. All users
must know the hazards presented by the device and the used substances.
The fraction collector is designed for laboratory research use in high-performance liquid
chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC)
applications. The fraction collector is part of the UltiMate 3000 system, but can also be
used with other systems. A control computer with RS-232 or USB port is required.
The fraction collector is controlled by the Chromeleon Chromatography Management
System.
Observe the following for using the fraction collector:
• The fraction collector has been designed for the multidimensional separation of
biomolecules with aqueous and polar organic mobile phases, for example methanol, that
are commonly used in reversed-phase (RP) liquid chromatography. For normal-phase (NP)
applications, observe the safety instructions for the fraction collector on page 4. For the
safety instructions in French, see page 7.
• The fraction collector may be operated only using the components originally supplied with
the unit and within the technical specifications and environmental conditions ( → page 71)
that are outlined in this manual.
• Use only standard solvents (HPLC grade, preferably LC-MS grade (0.2 µm filtered)) and
buffers, compatible with the flow path materials. Note any special properties of the
solvents such as viscosity, boiling point, UV absorption (UV/VIS detector), refractive
index (refractive index detector), and dissolved gas (degasser).
If there is any question regarding appropriate usage, contact Thermo Fisher Scientific before
proceeding.
Warning: If the device is used in a manner not specified by Thermo Fisher
Scientific, the protection provided by the device could be impaired.
Thermo Fisher Scientific assumes no responsibility and will not be
liable for operator injury and/or instrument damage. Whenever it is
likely that the protection is impaired, the instrument must be
disconnected from all power sources and be secured against any
intended operation.