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Thermo Scientific Accela UV/Vis Plus, Hardware Manual

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3

 Routine Maintenance

LightPipe Flow Cell Maintenance

46

Accela UV/Vis Detector Hardware Manual

Thermo Scientific

Cleaning the LightPipe Flow Cell with Organic Solvents

If you suspect that the flow cell needs to be cleaned, try cleaning it with organic solvents.

To clean the LightPipe flow cell with an organic solvent

1. Remove the column from the chromatographic system to avoid column degradation. 

2. Connect the flow cell inlet directly to the chromatographic pump outlet.

3. If necessary, flush the flow cell with water to prevent a reaction between the last solvent 

used in the chromatographic system and the cleaning solvent that you will use to clean 
the flow cell. 

4. Flush the flow cell with 40 to 50 mL of cleaning solvent, for example, isopropanol or 

methanol. 

5. Flush the flow cell with water to prevent a reaction between the cleaning solvent and the 

mobile phase that is used in your chromatographic application.

Cleaning the LightPipe Flow Cell with Nitric Acid

Methanol or isopropanol is generally sufficient for cleaning a flow cell. However, if the flow 
cell remains contaminated after flushing it with organic solvents, remove the flow cell from 
the detector and clean it with nitric acid.

IMPORTANT      

Ensure that the cleaning solvents you plan to use are miscible with 

the solvent already present in the flow cell and pump. Isopropanol is a good cleaning 
solvent for most applications. If the last solvent in the pump was an aqueous buffer 
solution, be sure to pump 25 to 40 mL of HPLC-grade water (or its equivalent) 
through the system to remove any salts before you flush the pump with the cleaning 
solvents. 

CAUTION  

 Do not use a syringe to force solvent through the flow cell. Pressurizing 

the flow cell can cause a leak or rupture and result in a dangerous and uncontrolled 
spraying of solvent.

CAUTION  

Nitric acid is a strong oxidizing acid, and it can react vigorously with alcohols 

(especially methanol). Be sure to wear protective clothing and eye protection and adhere 
to your company’s safety procedures for the proper handling and disposal of corrosive 
acids. Flush the flow cell with water to remove all traces of alcohol before flushing it with 
nitric acid.

Summary of Contents for Accela UV/Vis Plus

Page 1: ...Thermo Accela UV Vis Plus Detector Hardware Manual 60257 97005 Revision C December 2013 ...

Page 2: ... or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser This document is ...

Page 3: ...eripherals not specifically authorized and qualified by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and additional components options and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives Accela UV Vis Detector EMC Directive 2004 108 EC EMC compliance has been evaluated by TÜV Rheinland of ...

Page 4: ...s to the system and part replacement Accordingly order replacement parts from Thermo Fisher Scientific or one of its authorized representatives Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturi...

Page 5: ... Directives and the recyclers in your country WEEE Konformität Dieses Produkt muss die EU Waste Electrical Electronic Equipment WEEE Richtlinie 2002 96 EC erfüllen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder ents...

Page 6: ... compagnies de recyclage dans chaque état membre de l union européenne et ce produit devrait être collecté ou recyclé par celles ci Davantage d informations sur la conformité de Thermo Fisher Scientific à ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont disponi...

Page 7: ...es Gefahr durch entzündbare Substanzen Beachten Sie die einschlägigen Vorsichtsmaßnahmen wenn Sie das System in Gegenwart von entzündbaren Substanzen betreiben Peligro por sustancias inflamables Tenga mucho cuidado cuando utilice el sistema cerca de sustancias inflamables Danger lié aux substances inflammables agir avec précaution lors de l utilisation du système en présence de substances inflamma...

Page 8: ...s away from this area Quetschgefahr Halten Sie Ihre Hände von diesem Bereich fern Puntos de pinzamiento Mantenga las manos apartadas de esta área Risque de pincement éloigner les mains de cette zone Heavy objects Never lift or move the instrument by yourself you can suffer personal injury or damage the equipment For specific lifting instructions refer to the instrument manual Schweres Objekt Beweg...

Page 9: ...れる前に 加熱した部品を冷ましてください 热表面 待高温部件冷却之后再进行维修 Flammable substances hazard Use care when operating the system in the presence of flammable substances 可燃性物質の危険性 可燃性物質があるところでシステムを作動させる 場合は十分注意してください 易燃物危险 在有易燃物质的场地操作该系统时 务必小心谨慎 Risk of eye injury Eye injury could occur from splattered chemicals airborne particles or sharp objects Sharp objects that customers might install in the instrument include fused si...

Page 10: ...th the object 鋭利な物体 物体との身体的接触を避けてください 锋利物体 避免直接接触锋利的物体 Pinch point Keep hands away from this area ピンチポイント この部分には手を挟まれないようにしてください 夹点 勿将手放在此部位 Heavy objects Never lift or move the instrument by yourself you can suffer personal injury or damage the equipment For specific lifting instructions refer to the instrument manual 重量物 1 人で機器を持ち上げたり移動しないでください 身体傷害を 負ったり 機器を損傷するおそれがあります 具体的な持ち上げ方法 については 機器のマニュア...

Page 11: ...nt and Environmental Requirements 11 Installation Tools 12 Checking the Power Setting and Fuses 12 Installing the LightPipe Flow Cell 14 Connecting the Inlet and Outlet Tubing 14 Mounting the LightPipe Flow Cell 16 Installing the Standard Analytical Flow Cell 21 Making the Back Panel Connections 25 Connecting to the Data System Computer 26 Setting the Unit ID 27 Connecting the System Interconnect ...

Page 12: ...ning the LightPipe Flow Cell 45 Cleaning the LightPipe Flow Cell with Organic Solvents 46 Cleaning the LightPipe Flow Cell with Nitric Acid 46 Lamp Maintenance 48 Checking the Deuterium Lamp Usage Hours and Replacing the Lamp 48 Replacing the Tungsten Lamp 52 Replacing the Fuses 55 Chapter 4 Troubleshooting 57 Detector Related Problems 58 Error Messages 61 Appendix A Accessories and Replaceable Pa...

Page 13: ...sition settings for the detector as part of an instrument data acquisition method and turn the detector s lamps on and off Related Documentation In addition to this guide Thermo Fisher Scientific provides the following documents for the Accela UV Vis Detector Accela UHPLC System Preinstallation Requirements Guide Accela UHPLC System Getting Connected Guide Accela UHPLC User Guide for LC Devices Th...

Page 14: ...erts you to the presence of hot surfaces and to the potential injury that could occur were you to come in contact with a specific instrument area CAUTION Alerts you to the presence of an eye hazard and the requirement for wearing protective safety glasses CAUTION Alerts you to the presence of a UV light hazard IMPORTANT Highlights information necessary to prevent damage to software loss of data or...

Page 15: ...om en support landing support html To copy manuals from the Internet Go to mssupport thermo com agree to the Terms and Conditions and then click Customer Manuals in the left margin of the window To suggest changes to the documentation or to the Help Fill out a reader survey online at www surveymonkey com s PQM6P62 Send an e mail message to the Technical Publications Editor at techpubs lcms thermof...

Page 16: ......

Page 17: ... 1 Introduction The Accela UV Vis Plus Detector see Figure 1 is a member of the Accela family of UHPLC instruments Figure 1 Accela UV Vis Plus Detector Contents Functional Description LightPipe Flow Cells Lamps Front and Back Panels Status LEDs Specifications ...

Page 18: ...tector optical bench To provide a complete spectrum of ultraviolet and visible light the detector uses a deuterium lamp D2 for the UV range 190 to 365 nm and a tungsten lamp W for the visible range 366 to 800 nm The lamp cover has a safety interlock that reduces the possibility of human exposure to harmful UV light When you remove the cover the lamps automatically turn off A computer controlled sh...

Page 19: ...ide information about power communications run and lamp status See Status LEDs on page 7 LightPipe Flow Cells The UV Vis detector is available with a 10 mm 2 μL volume or 50 mm 10 μL volume LightPipe flow cell The mobile phase enters the LightPipe flow cell through the inlet port and exits through the outlet port Directing the flow from the bottom to the top of the flow cell reduces the developmen...

Page 20: ...aps until you install the LightPipe flow cell If you remove the LightPipe flow cell from the detector replace the protective end caps for storage Figure 5 End of the LightPipe flow cell Figure 6 50 mm LightPipe flow cell with protective end caps 50 mm flowpath Inlet port Alignment slot top Outlet port CAUTION Avoid touching the ends of the LightPipe flow cell Touching the ends of the flow cell can...

Page 21: ...t sensitivity allow a warm up period of 1 5 hours The tungsten lamp typically requires only 15 minutes of warm up time The deuterium lamp has a lifetime of approximately 2000 hours and the tungsten lamp has a lifetime of approximately 2500 hours Lamp lifetime varies depending on the application IMPORTANT The intensity of the deuterium lamp falls off very slightly over a period of time after it is ...

Page 22: ...n Jose CA 95134 U S A This device complies with FCC Rules Part 15 Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undesired operation DISCONNECT INPUT POWER BEFORE OPENING COVER REFER ALL SERVICE TO QUALIFIED PERSONNEL ONLY CAUTION T2 5A T1 25A 225VA 1...

Page 23: ... with the data system Run Green The detector is ready for a run Flashing green A run is in progress and the detector is sending data to the data system computer Flashing Amber An error has occurred during a run Lamps Green One or both lamps are turned on Amber The lamps are off Power Comm Run Lamps Table 2 UV Vis detector specifications Sheet 1 of 2 Item Specification Dimensions 18 36 47 cm h w d ...

Page 24: ... nm and 546 nm 10 mm LightPipe l flow cell Warm up time 90 minutes to meet noise and drift specifications Linearity Deviation 5 up to 2 0 AU at 257 nm Scan rate 4 5 6 67 10 or 20 Hz user selectable Rise time 0 0 1 1 2 3 4 or 5 seconds user selectable Flow cell dimensions and volume 10 mm 2 μL LightPipe flow cell 50 mm 10 μL LightPipe flow cell Flow cell pressure rating 1000 psi 69 bar Analog outpu...

Page 25: ...ist on page 10 and follow the procedures in this chapter Contents Installation Checklist Unpacking and Inspecting the UV Vis Detector Placement and Environmental Requirements Checking the Power Setting and Fuses Installing the LightPipe Flow Cell Installing the Standard Analytical Flow Cell Making the Back Panel Connections Turning On the UV Vis Detector for the First Time ...

Page 26: ... the UV Vis detector Installation step Reference page Unpack and inspect your instrument 11 Provide the proper location and environment 11 Gather the necessary tools 12 Check the power settings and fuses 12 Connect and install the LightPipe flow cell 14 Make the back panel connections 25 Turn on the UV Vis detector for the first time 37 This UV Vis detector was installed by Name Date ...

Page 27: ...e LightPipe Flow Cell Kits on page 64 Placement and Environmental Requirements Place the UV Vis detector on a benchtop as close as possible to the chromatographic column outlet This minimizes the length of tubing required to connect the LC column outlet to the LightPipe flow cell inlet If you are using other Accela modules place the UV Vis detector on top of the autosampler Ensure that the locatio...

Page 28: ...he small slot to the left of the fuse holder in the power entry module see Figure 11 and push to the right until the fuse holder pops out Figure 11 Power entry module 2 Pull the fuse holder out see Figure 12 Tool Accessory kit item Narrow blade screwdriver 2 mm wide No 2 Phillips screwdriver No 1 4 in open end wrench Yes 1 8 in ball end hex screwdriver Yes 3 32 in ball end hex screwdriver Yes 9 64...

Page 29: ...ove the voltage selector see Figure 13 and reinstall it so that the appropriate voltage label is upright and readable This voltage must agree with the incoming line voltage Figure 13 Voltage selector 5 Slide the fuse holder back into the power entry module until it snaps in IMPORTANT Do not connect the power cord or turn on the detector yet Wait until you perform the procedure in Turning On the UV...

Page 30: ...Cell Connecting the Inlet and Outlet Tubing Before you install the LightPipe flow cell in the detector connect the inlet and outlet tubing To connect the inlet and outlet tubing 1 Remove the protective end caps from the LightPipe flow cell Table 3 UV Vis detector configurations Part number Configuration 60057 60144 10 mm LightPipe flow cell 60057 60145 50 mm LightPipe flow cell Note A standard ana...

Page 31: ...utlet Note The insulating sleeve of the inlet tubing minimizes temperature fluctuations which cause baseline drift Note The 10 mm LightPipe flow cell is the same length as the 50 mm LightPipe flow cell so that it fits into the retaining block without the need for an adapter IMPORTANT If you have several detectors fluorescence refractive index electrochemical and so on hooked up in a series place y...

Page 32: ...nnect the other end of the blue 0 010 ID PEEK outlet tubing to the solvent waste container for the LC system Mounting the LightPipe Flow Cell To install the LightPipe flow cell 1 Open the front doors of the detector 2 Remove the flow cell cover as follows a Using the 1 8 in ball end hex screwdriver supplied unscrew the flow cell cover retaining screw see Figure 16 Figure 16 Flow cell cover retaini...

Page 33: ... assembly forward to maximize the space behind it to install the LightPipe flow cell 4 Remove the LightPipe flow cell retaining block as follows a Loosen and remove the thumbscrew holding the retaining block to the flow cell retaining bar see Figure 18 Figure 18 Flow cell mounting assembly b Pull the flow cell retaining block to the left and off the retaining bar Without the supporting retaining b...

Page 34: ...ar in the up position b Being careful not to touch the ends of the flow cell hold the flow cell with the alignment slot facing up see Figure 3 on page 3 and Figure 4 on page 4 as you insert it into the mounting assembly c Put the retaining bar down into the alignment slot in the flow cell see Figure 21 and Figure 22 Retaining bar Retaining bar CAUTION Do not touch the ends of the LightPipe flow ce...

Page 35: ...l d Make sure that the outlet tubing blue on the 10 mm LightPipe flow cell lies in a slot on the right side of the retaining block Figure 22 Retaining bar in the alignment slot on the 50 mm LightPipe flow cell 6 Reinstall the flow cell retaining block as follows a Align the retaining block with the notch in the base of the assembly see Figure 23 Figure 23 Retaining block in place Notch ...

Page 36: ...hex screwdriver supplied screw in and tighten the two photodiode assembly mounting screws see Figure 17 on page 17 8 Reinstall the flow cell cover as follows a Guide the tubing through the slots in the top and bottom of the flow cell compartment cover see Figure 25 Figure 25 Tubing slots in the flow cell compartment cover IMPORTANT To ensure optimum performance do not acquire data with the UV Vis ...

Page 37: ...low cell 1 Turn the detector off and unplug the power cord from line power 2 Do one of the following a If the LightPipe flow cell is not installed go to step 3 b If the LightPipe flow cell is installed remove it as described in Removing the LightPipe Flow Cell on page 42 and store it as described in Storing the LightPipe Flow Cell on page 45 Then go to step 4 3 Open the flow cell compartment and r...

Page 38: ... off the flow cell assembly Rest the photodiode mount out of the way inside the detector housing Figure 27 Figure 27 Photodiode mount removed d Using the 9 64 in ball end hex screwdriver supplied remove the two screws securing the LightPipe flow cell mounting assembly to the detector see Figure 27 5 Store the LightPipe flow cell mounting assembly in a secure place for possible future use The mount...

Page 39: ... b Using the 9 64 in ball end hex screwdriver supplied and the two screws removed from the LightPipe flow cell mounting assembly secure the standard analytical flow cell assembly to the detector see Figure 29 Figure 29 Standard analytical flow cell mounted to the detector c Position the photodiode assembly with the mounting holes aligned with the two standoffs on the flow cell assembly see Figure ...

Page 40: ...hotodiode assembly in place d Secure the tubing with the tubing clamp see Figure 31 Figure 31 Tubing secured with tubing clamp e Position the standard analytical flow cell cover over the flow cell assembly routing the tubing through the slots in the left side of the cover and secure the cover with the 10 32 0 75 in diameter 0 5 in length thumbscrew fS in the accessory kit see Figure 32 Photodiode ...

Page 41: ...ct cable that transmits the start signal from the autosampler to the detector during an injection cycle as described in Connecting the System Interconnect Cable or Cables If you are using an autosampler that is not controlled from your Thermo Scientific data system use the remote communications pins on the detector s back panel to trigger the detector as described in Making Remote Communications C...

Page 42: ... Vis detector to the data system computer 1 Plug one end of an Ethernet cable into the Ethernet port on the back panel of the UV Vis detector and plug the other end of the cable into the Ethernet switch Figure 33 2 Connect the Ethernet switch to the data system computer with a supplied Ethernet cable Figure 33 Ethernet connections USB ENET Solvent platform Accela UV Vis Detector Accela Autosampler...

Page 43: ...refer to the data system Help Use a small flathead screwdriver to change the setting of the rotary switches Figure 34 Unit ID rotary switches set to a value of 01 Connecting the System Interconnect Cable or Cables The timing of the Accela LC modules is synchronized through a system interconnect cable or cables The contact closure cabling system depends on the autosampler model To make the contact ...

Page 44: ...tector as shown in Figure 9 on page 6 2 To connect an autosampler plug the unlabeled connector into the left 8 pin receptacle on the back panel of the autosampler as shown in Figure 36 3 To connect an Accela pump all models plug a connector labeled PUMP into the 8 pin receptacle on the back panel of the pump DETECTOR DETECTOR DETECTOR PUMP PUMP A S M S To MS detector To autosampler To detector To ...

Page 45: ... 5V 150mA GND 1 2 3 4 5 6 7 8 A S READY INJECT OUT INJECT HOLD PUMP READY GRAD START PUMP STOP 5V 150mA GND 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 ENET RS232 UNIT ID 910111213141516 GND B CHB 1V CHB 100mV CHB 10mV GND A CHA 1V CHA 100mV CHA 10mV 1 2 3 4 5 6 7 8 EVENT GND ZERO READY RUN NC 5V 150mA GND 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 Connector labeled DETECTOR Connector labeled PUMP System...

Page 46: ...hows the adapter cable for an Accela detector Tip If the accessory kit for your Accela Open Autosampler does not contain a detector adapter cable order the cable from Thermo Fisher Scientific The Accela Open Autosampler system interconnect cable supplied with the autosampler does not have any labels Table 4 Accela Open Autosampler contact closure and adapter cables Cable description Part number Us...

Page 47: ... into 25 socket connector of the Accela Pump adapter cable see Figure 40 and plug the other end of the adapter cable into the D Sub25 receptacle on the pump s back panel 2 To connect the adapter cable to the interconnect cable plug the 2 pin male connector labeled TO 60157 63024 on the adapter cable into the 2 pin female connector on the interconnect cable 3 Connect the adapter cable connector lab...

Page 48: ... or 1250 Pump ENET UNIT ID GND B CHB 1V CHB 100mV CHB 10mV GND A CHA 1V CHA 100mV CHA 10mV EVENT GND ZERO READY RUN NC 5V 150mA GND 910111213141516 1 2 3 4 5 6 7 8 RS232 D SUB25 INTERFACE 1 TO 60157 63024 60157 63026 TO LCQ FLEET TO LTQ LXQ MSQ TO TSQ EXACTIVE TO PDA TO MSQ LTQ LXQ TO PUMP 60157 63024 TO A S Accela 600 Pump or Accela 1250 Pump Accela UV Vis Detector Accela Open Autosampler Interco...

Page 49: ... A CHA 1V CHA 100mV CHA 10mV EVENT GND ZERO READY RUN NC 5V 150mA GND 910111213141516 1 2 3 4 5 6 7 8 RS232 INTERFACE 1 PRESSURE GND INJECT HOLD PUMP READY GRADIENT START PUMP STOP 5V GND 1 2 3 4 5 6 7 8 TO MSQ LTQ LXQ 60157 63024 TO A S TO PUMP TO PUMP TO 60157 63024 60157 63022 TO 60157 63024 60157 63026 TO LCQ FLEET TO LTQ LXQ MSQ TO TSQ EXACTIVE TO PDA Accela Pump Accela UV Vis Detector Accela...

Page 50: ...V Vis detector CHA 10 mV CHA 100 mV CHA 1 V CHB 10 mV CHB 100 mV CHB 1 V and a single ground per channel GND A and GND B To make each electrical connection 1 Insert the end of the wire into the appropriate terminal in the 8 pin terminal connector Hold the wire in place while you tighten the small terminal set screw firmly onto the wire 2 Insert the terminal connector into the 8 pin analog connecto...

Page 51: ...ampler or from a manual injector and sends a run start signal to the data system software During installation you must connect the RUN input on the UV Vis detector to your autosampler or injector output To make this contact closure connection do the following If your LC system contains an Accela Autosampler use the 7 connector interconnect cable If your LC system contains an Accela Open Autosample...

Page 52: ...vice output relay consists of a single normally open terminal and a common ground terminal connect the normally open terminal to the RUN terminal on the UV Vis detector and connect the common ground terminal to the GND terminal on the UV Vis detector If both of the relay contacts are electrically isolated from each other and from ground the device simply closes a contact between the two relay term...

Page 53: ... connected to the data system computer 3 Check the fuse size You can operate the UV Vis detector at 100 115 V or 230 V However you must ensure that the appropriate fuses are installed see Checking the Power Setting and Fuses on page 12 4 Plug the power cord into the power receptacle on the back panel of the detector see Figure 9 on page 6 and connect it to the power source 5 Turn the power on by p...

Page 54: ......

Page 55: ...lar basis If you have any questions on proper maintenance or would like to arrange for a preventive maintenance program contact your Thermo Fisher Scientific field service engineer To maintain your UV Vis detector follow the routine maintenance procedures in this chapter Contents Recommended Routine Maintenance Preventive Maintenance Tools Required Cleaning the External Surfaces of the Detector Li...

Page 56: ...xternal surfaces As needed See Cleaning the External Surfaces of the Detector on page 41 Cleaning the flow cell As needed a See Cleaning the LightPipe Flow Cell on page 45 a Flushing the flow cell with clean solvent after every use will reduce how often you need to clean it Lamp replacement Deuterium D2 Replace the deuterium lamp when the detector noise reaches an unacceptable level See Lamps on p...

Page 57: ...water or methanol If this does not remove the contamination clean the flow cell with a dilute solution of nitric acid followed by a water rinse Thoroughly flush and clean all peripheral plumbing pumps and so on before connecting them to the flow cell To avoid particulate contamination and fluid path blockage maximize the use of filtration Avoid leaving buffers standing in the flow cell for long pe...

Page 58: ...htPipe flow cell 1 Open the front doors of the detector 2 Using the 1 8 in ball end hex screwdriver supplied unscrew the flow cell cover retaining screw see Figure 43 Figure 43 Flow cell cover retaining screw CAUTION Do not disassemble the LightPipe flow cell housing or tighten the screws on the housing Doing so will damage the LightPipe flow cell Thermo Fisher Scientific cannot be held responsibl...

Page 59: ...e 44 Figure 44 Photodiode assembly mounting screws 5 Move the photodiode assembly forward to maximize the space behind it to install the LightPipe flow cell 6 Loosen and remove the thumbscrew holding the retaining bar to the flow cell retaining block see Figure 45 CAUTION Do not touch the surface of the photodiode When removing the photodiode mount be sure to place the mount where the photodiode w...

Page 60: ...ll Maintenance 44 Accela UV Vis Detector Hardware Manual Thermo Scientific Figure 45 Flow cell mounting assembly 7 Remove the flow cell retaining block see Figure 46 Figure 46 Retaining block removed Retaining block Thumbscrew Retaining bar ...

Page 61: ... varies or the cleanliness of your samples varies Signs of a contaminated flow cell are increased baseline noise signal spiking erratic or drifting baselines low light intensity or increased backpressure To clean the LightPipe flow cell follow these procedures Cleaning the LightPipe Flow Cell with Organic Solvents Cleaning the LightPipe Flow Cell with Nitric Acid CAUTION Avoid touching the ends of...

Page 62: ...sopropanol is generally sufficient for cleaning a flow cell However if the flow cell remains contaminated after flushing it with organic solvents remove the flow cell from the detector and clean it with nitric acid IMPORTANT Ensure that the cleaning solvents you plan to use are miscible with the solvent already present in the flow cell and pump Isopropanol is a good cleaning solvent for most appli...

Page 63: ... 20 v v solution of nitric acid in HPLC grade water 6 Using the chromatographic pump pump the nitric acid solution through the flow cell 7 After you have finished the cleaning procedure and before you return to the chromatographic solvents pump another 25 to 40 mL of water through the flow cell to remove all traces of nitric acid Monitor the pH of the outlet stream of the flow cell to ensure that ...

Page 64: ...500 hours Lamp lifetime varies depending on the application To access the lamps open the front doors of the detector The deuterium and tungsten lamps are located in the housing on the left The lamps are supplied pre aligned in their individual assemblies to make them easy to install and align To replace the detector s lamps follow these procedures Checking the Deuterium Lamp Usage Hours and Replac...

Page 65: ...ning screw on the lamp cover Figure 48 Figure 48 Lamp cover retaining screw 4 Pull the cover off to expose the lamp assemblies Because intense UV light can damage your eyes the lamps automatically turn off when you turn off the detector or remove the lamp cover CAUTION Turn the detector off and unplug the power cord from the power receptacle on the back panel as a secondary precaution against the ...

Page 66: ... the deuterium lamp cover as described in Accessing the Deuterium Lamp and Reading Its Chronometer on page 49 2 Gently unplug the deuterium lamp lead from the detector see Figure 51 Take care not to twist the connector Note Two mercury columns separated by a small electrolyte gap fill the glass capillary tubing that is visible in the chronometer window With lamp usage the mercury column on the lef...

Page 67: ...the lamp assembly in place and pull the assembly straight out Installing a New Deuterium Lamp To install a new deuterium lamp 1 Remove the used deuterium lamp as described in Removing the Deuterium Lamp on page 50 2 Hold the deuterium lamp assembly so that the leads are at the top 3 Slide the assembly onto the alignment pin see Figure 52 Deuterium lamp lead Thumbscrews Deuterium lamp ...

Page 68: ...g the power cord back into the power receptacle on the back panel of the detector Replacing the Tungsten Lamp To replace the tungsten lamp follow these steps 1 Removing the Tungsten Lamp 2 Installing a New Tungsten Lamp Removing the Tungsten Lamp To remove the tungsten lamp 1 Turn the detector off unplug the power cord from the power receptacle on the back panel and allow sufficient time for the l...

Page 69: ... 51 Take care not to twist the connector Figure 53 Tungsten lamp assembly 6 Loosen and remove the thumbscrew that holds the lamp assembly in place 7 Pull the assembly straight out and remove it from the detector CAUTION Because intense UV light can damage your eyes the detector automatically turns off the lamps when the lamp cover is removed CAUTION Because the lamps run hot turn off the power and...

Page 70: ...stall a new tungsten lamp 1 Remove the used tungsten lamp as described in Removing the Tungsten Lamp on page 52 2 Hold the lamp assembly so that the leads are at the top Slide the assembly onto the two alignment pins shown in Figure 55 The alignment pins are located on either side of the detector monochromator aperture Figure 55 Alignment pins for the tungsten lamp Alignment holes Tungsten bulb Al...

Page 71: ...Vis detector does not power up you might need to replace the fuses To replace the fuses 1 Turn off the power to the detector and unplug the detector from line power 2 Place the tip of the narrow blade screwdriver in the small slot to the left of the fuse holder in the power entry module see Figure 56 and push to the right The fuse holder pops out Figure 56 Power entry module 3 Pull the fuse holder...

Page 72: ...a UV Vis Detector Hardware Manual Thermo Scientific 4 Remove the fuses from the fuse holder see Figure 58 and replace them with new ones Figure 58 Fuses in the fuse holder 5 Slide the fuse holder back into the power entry module until you hear a click ...

Page 73: ...a table of symptoms possible causes and remedies see Table 6 Because many of the problems attributed to the detector might actually be due to other components in the chromatographic system this table also includes references and potential solutions to these types of problems To aid in troubleshooting errors Table 7 lists some common error messages and numeric codes Contents Detector Related Proble...

Page 74: ...les following mobile phase changeover Flush the flow cell with 2 propanol and then with mobile phase Electrical interference Check the electrical lines for good connections interference from broadcast radiation or both Check for ground loops Extremely large voltage fluctuations in line power Remove systems that cause voltage fluctuations for example ovens isolate the detector to a quiet circuit or...

Page 75: ...the dirty component See Cleaning the LightPipe Flow Cell on page 45 Integrator input and output voltage not matching Verify that the integrator is connected to the appropriate analog output connections on the back panel of the detector See Connecting the Analog Outputs on page 34 Check the attenuation setting on the integrator Excessive baseline drift Contaminated flow cell Flush the flow cell wit...

Page 76: ...ime selection Poor connection at flow cell inlet Check the end of the inlet tubing for a clean flat surface free of obstructions Detector not powering up Tripped circuit breaker at power outlet Reset the circuit breaker Blown detector fuse Replace the fuse See Replacing the Fuses on page 55 Incorrect voltage selected Verify that the voltage selector is set for the correct incoming line voltage See...

Page 77: ...set 2002 System error bus address 2004 Lamp case open 2011 Sample light is too high 2012 Low light on D2 lamp 2013 Low light on W lamp 2014 Volatile RAM test failed 2015 Non Volatile RAM test failed 2016 12 volts failed 2017 12 volt unregulated failed 2018 12 volts failed 2019 Motor voltage failed 2020 W lamp voltage or driver failed 2021 D2 lamp or driver failed 2022 5 volts failed 2023 Reference...

Page 78: ...p off option 2212 Invalid program time 2213 Invalid wavelength for program 2215 Invalid program type 2216 Invalid output range 2217 Invalid rise time filter 2218 Invalid auto zero time 2219 Invalid output range 2220 Invalid rise time filter 2221 Invalid run wavelength 2226 Invalid auto zero time 2227 Invalid rise time filter 2228 Invalid run time 2232 Invalid runs per wavelength 2234 Invalid offse...

Page 79: ...its Standard Analytical Flow Cell Parts Maintenance Parts Service Spare Parts Table 8 Accela UV Vis Detector accessory kit P N 60053 62004 Sheet 1 of 2 Part number Description Quantity 6040 0102 Cable external run auto zero 1 00004 02511 8 pin cable connector 3 81 mm pitch Mini Combicon 2 70111 63302 Cable RJ 45 Ethernet communication CAT 5 shielded 1 6040 0103 Cable analog DET 2 3256 0024a Nut 1 ...

Page 80: ...ck 1 5401 0400 Open end wrench 1 4 5 16 in 1 00109 02 00029 One piece 10 32 PEEK fitting for 1 16 in OD tubing 17 mm length Valco P N JR 55100 5 2 2816 4210 Thumbscrew 10 32 0 75 in diameter 0 5 in stud length for the standard analytical flow cell cover 1 a Upchurch part number P 200 includes the nut and ferrule P N 3256 0024 and 3256 0025 Table 8 Accela UV Vis Detector accessory kit P N 60053 620...

Page 81: ...pe flow cell 2522 0285 Fingertight 10 32 one piece PEEK fitting 803260 Inlet tubing with insulation PEEK 1 16 in OD 0 005 in ID red 33 in length 703950 Outlet tubing PEEK 1 16 in OD 0 01 in ID blue 60 in length Table 11 Standard analytical flow cell parts Part number Description 60053 40005 Standard flow cell covera a The thumbscrew for securing the cover is located in the accessory kit See Table ...

Page 82: ...63S Cuvette holder assembly 6040 0167 Deuterium lamp cable 2110 0354 External connector 9551 0200 High voltage transformer 60053 40042 Lamps cover F5025 010 LED cable 60053 40041 LightPipe flow cell cover 5110 0058 Magnetic proximity switch 9551 0187 04 Main transformer 60053 61079R Motherboard PCB 60053 63031 Photodiode pre amp assembly F3030 010 Power entry module with switch 70111 63302 RJ 45 C...

Page 83: ...terconnect Accela Autosampler 27 Accela Open Autosampler 30 chromatograms acquiring with an A D converter 34 column bleed 59 compliance FCC iv regulatory iii WEEE v connections analog output 34 contact closure 27 data system computer 26 LightPipe flow cell 14 remote communications inputs 35 contacting us xv D data system computer connecting 26 detectors multiple 15 deuterium lamp description 5 ins...

Page 84: ...linearity 8 M maintenance preventive 41 recommendations for 40 tools 41 multiple detectors placement 15 N noise causes of 59 O operating conditions humidity 7 temperature 7 optical bench 2 P peak problems broadening 60 size 60 tailing 60 power requirements 7 R regulatory compliance iii remote controls 8 rise time incorrect setting results of 60 specification 8 S safety standards iii scan data rate...

Page 85: ...x U tungsten lamp description 5 installing 54 lifetime 5 48 removing 52 U uninterruptible power supply UPS 58 unit ID setting 27 V voltage ac line problems 58 voltage selector 12 55 W warm up time 8 wavelength accuracy and range 8 WEEE compliance v weight detector 7 ...

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