SMARTer™ ICELL8® cx Single-Cell System User Manual
(061918)
takarabio.com
Takara Bio USA, Inc.
Page 3 of 35
D. Bi-Annual Preventative Maintenance ....................................................................................................................... 33
X. Troubleshooting ............................................................................................................................................................ 34
A. Technical Support ..................................................................................................................................................... 35
Table of Figures
Figure 1. The ICELL8 cx instrument. ................................................................................................................................... 10
Figure 2. Water Bottle seated on the Electronic Scale. ......................................................................................................... 11
Figure 3. Humidifier unit. ..................................................................................................................................................... 11
Figure 4. Syringe Pumps Unit. .............................................................................................................................................. 12
Figure 5. ICELL8 cx Stage Module. ..................................................................................................................................... 12
Figure 6. ICELL8 cx workflow. ............................................................................................................................................ 15
Figure 7. Example of DewPoint parameter display. ............................................................................................................. 17
Figure 8. Wash Container. .................................................................................................................................................... 18
Figure 9. Startup tab. ............................................................................................................................................................. 18
Figure 10. Daily checklist. .................................................................................................................................................... 18
Figure 11. The [Tip Clean] button in the Manual Control section. ....................................................................................... 19
Figure 12. Source plate map. ................................................................................................................................................ 20
Figure 13. Single Cell / TCR tab. .......................................................................................................................................... 20
Figure 14. Placing the chip on the Dispensing Platform. ...................................................................................................... 21
Figure 15. Empty plate nest for the sample source plate. ..................................................................................................... 21
Figure 16. View of the ICELL8 cx with the front door closed and open. ............................................................................. 22
Figure 17. Select the 3’DE / TCR tab. .................................................................................................................................. 22
Figure 18. Vacuum status confirmation. ............................................................................................................................... 23
Figure 19. Confirm plate and chip loading. .......................................................................................................................... 23
Figure 20. Confirm seal removal. ......................................................................................................................................... 23
Figure 21. Live image of chip scanning. ............................................................................................................................... 24
Figure 22. Input chip ID. ....................................................................................................................................................... 24
Figure 23. Vacuum status after dispense. ............................................................................................................................. 24
Figure 24. Initiate chip scanning. .......................................................................................................................................... 25
Figure 25. Remove the chip seal. .......................................................................................................................................... 25
Figure 26. Enter the chip ID after autofocus and barcode scanning steps. ........................................................................... 26
Figure 27. Select analysis setting and barcode gal files. ....................................................................................................... 26
Figure 28. Select path and file name for saving data. ........................................................................................................... 27
Figure 29. Naming and saving results file. ........................................................................................................................... 27
Figure 30. Cell / RT dispense and scanning. ......................................................................................................................... 29
Figure 31. RT-PCR mix Source Plate map. .......................................................................................................................... 29
Figure 32. Initiate RT dispense. ............................................................................................................................................ 29
Figure 33. Loading filter files. .............................................................................................................................................. 30
Figure 34. Confirm removal of sealing film. ........................................................................................................................ 31
Figure 35. Live image of chip scanning. ............................................................................................................................... 31
Figure 36. Enter chip ID. ...................................................................................................................................................... 32
Figure 37. Confirm that chip ID matches filter file name. .................................................................................................... 32
