SMARTer™ ICELL8® cx Single-Cell System User Manual
(061918)
takarabio.com
Takara Bio USA, Inc.
Page 28 of 35
F.
Blotting and Sealing the Chip
1.
After imaging is complete, promptly blot the chip for 2 sec, as described below.
a.
Place the chip, wells facing up, on a clean lab wipe.
b.
Gently place a piece of blotting paper directly on top of the chip. Make sure that the blotting
paper covers the entire face of the chip.
c.
Pick up the blotter by the top handle and place the flat face of the blotter against the blotting
paper on the chip. The blotter should extend beyond the edges of the chip.
d.
Let the blotter rest on top of the blotting paper for exactly 2 sec without pressing down; the
weight of the blotter is sufficient for adequate blotting.
e.
Remove the blotter, then gently remove the blotting paper and dispose of it in a biohazard
container.
2.
Quickly seal the loaded chip with Intermediate Film.
3.
Centrifuge as described below.
a.
Place your chip on the centrifuge tray.
b.
Counterbalance with a second chip.
c.
Centrifuge at 300
g
for 5 min at 20–25°C.
NOTE:
Small bubbles on the periphery of the chip will not cause problems, but bubbles on top of
nanowells will.
